Marine and Freshwater Research Marine and Freshwater Research Society
Advances in the aquatic sciences

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Inoculating Pseudomonas putida promotes submerged plant Vallisneria natans growth via carbon conversion in a plant-microbe interaction

Lin Gan , Hui Zhao , Aili Wang , Sanshan Li , Jia Liu , Liuyan Yang

Abstract

Submerged plant growth is limited by the reduction of underwater photosynthesis attributed to low CO2 availability as well as light limitation associated with underwater conditions. Heterotrophic bacteria and fungi play an important role in local aqueous DIC content surrounding submerged plants. In order to investigate plant growth influenced by carbon conversion in plant-microbe interactions, we inoculated the plant medium of Vallisneria natans with Pseudomonas putida KT2440 and measured carbon conversion in the system as well as several indices of plant growth. Our results showed that the quantity of P. putida KT2440 increased twofold due to the availability of organic matter produced by V. natans. Similarly, P. putida KT2440 supplied DIC for V. natans, improving its photosynthesis rate. Moreover, the significantly higher leaf area, specific leaf area and fresh biomass attributed to the presence of P. putida KT2440 demonstrated the interaction enhanced the efficiency of nutrient and CO2 uptake by V. natans, promoting V. natans growth. Therefore, we suggest that the micro-cycle of carbon and oxygen based on the protocooperation of V. natans and P. putida KT2440 may accelerate the transformation of carbon to increase the carbon availability to promote the growth of both plant and microbe.

MF17117  Accepted 12 January 2018

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