Register      Login
Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
Shopping Cart: ( empty )
You are here: Home > Journals > RD > RD05069
RESEARCH ARTICLE
Contents Vol 18(5)

Effect of enhancement of uterine involution and earlier initiation of post-partum cyclicity on the reproductive performance of buffalo

A. A. Ramoun A D , S. A. Darweish B , H. A. Abou El-Ghait C and El-S. M. Fattouh A
+ Author Affiliations
- Author Affiliations

A Theriogenology Department, Faculty of Veterinary Medicine in Kafr El-Sheikh, Tanta University, Egypt.

B Animal Production Research Institute, Mahallet Mousa Buffalo Research Farm, Kafr El-Sheikh, Egypt.

C The General Authority of Veterinary Services, El-Gharbia Province, Egypt.

D Corresponding author. Email: ramoun2004@yahoo.com

Reproduction, Fertility and Development 18(5) 545-550 https://doi.org/10.1071/RD05069
Submitted: 23 June 2005  Accepted: 7 March 2006   Published: 24 May 2006

Abstract

The aim of the present work was to study the effect of either gonadotrophin-releasing hormone (GnRH)–prostaglandin PGF2α–GnRH (G–P–G) or equine chorionic gonadotrophin (eCG)–PGF2α–GnRH (eCG–P–G) regimes with or without intramuscular injection of ergometrine maleate (Methergin) within 24 h post partum on the uterine involution, initiation of post partum cyclicity and subsequent reproductive performance of buffalo. A total of 60 parturient Egyptian buffalos (2–4 lactations) were used to conduct this study. The involved animals were allotted into two main groups (A and B), each with 30 animals, on the basis of whether buffalo cows received an i.m. injection of 3 mg of Methergin within the first 24 h post partum (A, Meth. T) or not (B, Meth. NT). Each main group was subdivided into three subgroups: A1, A2 and A3 in case of A and B1, B2 and B3 in case of B. The G–P–G regime was applied on both A1 (Meth. T) and B1 (Meth. NT) buffalos, where each individual received two i.m. injections of 0.020 mg GnRH analogue (buserelin) at Days 18 and 33 post partum with i.m. injection of 25 mg PGF2α (Dinoprost) in between at Day 25. The eCG–P–G regime was applied on both A2 (Meth. T) and B2 (Meth. NT) buffalos, where each individual received i.m. injection of 1000 IU eCG, 25 mg of PGF2α (Dinoprost) and 0.020 mg of GnRH analogue (buserelin) at Days 8, 18 and 25 post partum, respectively. The A3 subgroup (Meth. T, alone) was control for A1 and A2 subgroups and the B3 subgroup was the non-treated control for all subgroups. There was a significant (P < 0.01) decrease in the days required for complete uterine involution in subgroups A1, B1 and A3, indicating the enhancement of uterine involution. The post partum cyclicity was initiated earlier in all of the treatment regimes applied in the present study, as was indicated by significant (P < 0.01) decrease in the days to first ovulation in these subgroups compared with the non-treated control subgroup. The reproductive performance of treated buffalos improved as was shown by the significant (P < 0.01) decrease in days to first service, days open and calving interval. It is concluded that enhancement of uterine involution and/or earlier initiation of post-partum cyclicity improve the reproductive performance of buffalos.

Extra keywords: eCG–P–G regime, G–P–G regime, Methergin.


References

Abraham G. E. (1981) The application of natural steroid radioimmunoassay to gynaecologic endocrinology. In ‘Radioassay Systems in Clinical Endocrinology’. (Ed. G. E. Abraham.) pp. 475–529. (Marcel Dekker: Basel, Germany.)

Agarwal S. K., and Tomar O. S. (1998). ‘Reproductive Technologies in Buffaloes.’ (Indian Veterinary Research Institute: Izatnagar, India.)

APRI (1997). ‘Animal and Poultry Nutrition.’ (Animal Production Research Institute, Ministry of Agriculture: Giza, Egypt.) [In Arabic]

Bagal, S. B. , and Kadu, M. S. (1989). Effect of GnRH analogue (Buserelin) on postpartum reproductive performance in cross-bred cows. Ind. J. Anim. Sci. 59, 1534–1535.
Braun R. K., Noordhuizen J. P., Donovan G. A., and Brand A.(1982). A goal oriented approach to herd health and production control. In ‘Proceedings of the 15th Annual Convention of the American Association of Bovine Practitioners December 1–4’.

Britt, J. H. (1975). Early postpartum breeding in dairy cows. A review. J. Dairy Sci. 58, 266–271.
| PubMed | Drost M. (1987). Clinical uses of hormones. In ‘Society for Theriogenology, Cow Manual’. (Ed. A. Bruce.) pp. 73–80. (Texas Veterinary Medicine Diagnostic Laboratory: College Station, TX, USA.)

Ebling, F. J. , Brooks, A. N. , Cronin, A. S. , Ford, H. , and Kerr, J. B. (2000). Estrogenic induction of spermatogenesis in the hypogonadal mouse. Endocrinology 141, 2861–2869.
| Crossref | GoogleScholarGoogle Scholar | PubMed | El-Sabbagh K. M. A. (1993). A study on some biochemical constituents of blood female buffaloes during late pregnancy and puerperium. M.V.Sc. Thesis, Zagazig University, Faculty of Veterinary Medicine, In Moshotohor, Egypt.

El-Weishy, A. B. , and El-Sawaf, S. A. (1970). Reproduction in buffaloes in Egypt III – Service period and its components. Anoderdruck Aus Zertschirift für Tierzüchtung und Züchtungs BioloAe 87, 325–334.
Pineda M. H. (1989). Female reproductive system. In ‘McDonals’s Veterinary Endocrinology and Reproduction’. (Eds M. H. Pineda and M. P. Dooley.) pp. 283–340. (Iowa State Press: Ames, IA, USA.)

Qureshi, M. S. , Samad, H. A. , Nazir Ahmad Habib, G. , Anjum, A. D. , and Siddiqui, M. M. (1998). Reproductive performance of dairy buffaloes under Peri-Urban Commercial farming in NWFP, Pakistan. Pakistan Vet. J. 18, 197–202.
Robert S. J. (1986). ‘Veterinary Obstetric and Genital Diseases.’ (Published by the author.)

Roche, J. F. , Diskin, M. G. , and Bergamini, P. F. (2000). Resumption of reproductive activity in the early postpartum period of cows. Atti-della Soieta-Itliana-di-Buiatria 32, 33–47.
SAS (1985). ‘SAS User’s Guide: Statistics.’ (SAS Institute: Cary, NC, USA.)

Schirar A., and Martinet J. (1982). Post-partum ovarian activity and its interaction with the uterus in resuming cyclic activity post-partum. In ‘Factors Influencing Fertility in the Post-partum Cow’. (Eds H. Karg and E. Schallenberger.) pp. 67–94. (Martinus Nijhoff: the Hague, the Netherlands.)

Schultz R. H. (1987a). Objective evaluation of herd reproductive performance. In ‘Society for Theriogenology, Cow Manual’. (Ed. A. Bruce.) pp. 157–161. (Texas Veterinary Medicine Diagnostic Laboratory: College Station, TX, USA.)

Schultz R. H. (1987b). Synchronization of ovulation/estrus: Prostaglandin/prostaglandin analogues and GnRH. In ‘Society for Theriogenology, Cow Manual’. (Ed. A. Bruce.) pp. 104–113. (Texas Veterinary Medicine Diagnostic Laboratory: College Station, TX, USA.)

Shah, S. N. H. , Willemse, A. H. , and Van-DeWiel, D. F. (1990). Reproductive performance of Nili-Ravi buffaloes after a single injection of GnRH early postpartum. Trop. Anim. Health Prod. 22, 239–246.
| Crossref | GoogleScholarGoogle Scholar | PubMed | Shawki G. (1983). Some investigations on postpartum period in buffaloes. M. Vet. Sci. Thesis, Zagazig University, Zagazig, Egypt.

Sheldon, I. M. , and Dobson, H. (2000). Effect of administration of eCG to postpartum cows on folliculogenesis in the ovary ipsilateral to the previously gravid uterine horn and uterine involution. J. Reprod. Fertil. 119, 157–163.
| Crossref | GoogleScholarGoogle Scholar | PubMed | Vale W. G., Ohashi O. M., Sousay J. S., and Ribeiro H. F. (1990). Studies on the reproduction of water buffalo in the Amazon basin. In ‘Livestock Reproduction in Latin America’. pp. 201–210. (FAO/LAEA Semina: Bogota, Colombia.)

Van De Plassch, M. (1981). Neve Vergleichende aspekte derinvolution und der puerperalen meteritis Bei state, Huh Sau, Mh. Vet. Med. (Praha) 36, 804–807.