Nuclear magnetic resonance spectroscopy of bovine ovarian follicular fluid at four selected times of the oestrous cycle
Gordon E. Sarty A B E , Edward J. Kendall B , Gregg P. Adams C and Roger A. Pierson DA Department of Psychology, College of Arts and Science, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
B Department of Medical Imaging, College of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
C Department of Veterinary Medical Biosciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
D Department of Obstetrics, Gynaecology and Reproductive Sciences, College of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
E Corresponding author. Email: gordon.sarty@usask.ca
Reproduction, Fertility and Development 18(5) 559-571 https://doi.org/10.1071/RD06010
Submitted: 9 February 2006 Accepted: 15 March 2006 Published: 24 May 2006
Abstract
The objective of the study was to determine if nuclear magnetic resonance (NMR) spectral features of ovarian follicular fluid were correlated with the physiological status of follicles so that we could assess the feasibility of using NMR spectroscopy during assisted reproduction therapy. Thirty-five sexually mature, nullparious heifers were monitored by transrectal ultrasonography to assess their follicle wave status during the oestrous cycle. Ovariectomies were performed on Day 3 of wave 1 (D3W1, n = 10), Day 6 of wave 1 (D6W1, n = 9), Day 1 of wave 2 (D1W2, n = 9), or in the immediate preovulatory period of at least 17 days after ovulation (D≥17, n = 9). Follicle status was determined to be dominant or subordinate. Follicular fluid was extracted from the follicles and NMR spectra were collected. Principal components were extracted from ratios of line amplitudes and tested for effects of follicle status (dominant v. subordinate) and cycle time point (D1W3, D1W6, D1W2 and D≥17) using multivariate analysis of variance. For most line ratio combinations, main effects of status, time point and their interaction were found (P < 0.05). We concluded that NMR spectra may be used for the determination of ovarian follicle physiological status.
Extra keywords: assisted reproduction technology, bovine, follicle, ovary, spectroscopy.
Acknowledgments
Funding for this project was provided by the Canadian Institute for Health Research (CIHR) and the Natural Sciences and Engineering Research Council (NSERC) of Canada. Thanks are given to Jennifer Hadley who quantified the spectral data before statistical analysis.
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