Expression and regulation of lanosterol 14α-demethylase in mouse embryo and uterus during the peri-implantation period
Xiaoming Song A , Ping Tai A , Jun Yan A , Baoshan Xu A , Xiufen Chen A , Hong Ouyang A , Meijia Zhang A and Guoliang Xia A BA State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100094, P. R. China.
B Corresponding author. Email: glxiachina@sohu.com
Reproduction, Fertility and Development 20(8) 964-972 https://doi.org/10.1071/RD08085
Submitted: 25 April 2008 Accepted: 4 August 2008 Published: 23 October 2008
Abstract
Lanosterol 14α-demethylase (LDM) is expressed ubiquitously in all mammals and is important in cholesterol biosynthesis. However, whether LDM expression is involved in the interaction between uterus and embryo during implantation remains unknown. In the present study, the expression of LDM was investigated in mouse embryo and uterus during the peri-implantation period using confocal microscopy, immunohistochemistry and western blot methods. Further, regulation of LDM expression was investigated in pseudopregnancy, delayed implantation, artificial decidualisation and ovariectomisation using 17β-oestradiol and progesterone treatment mouse models. The results showed that LDM was selectively expressed in preimplantation embryos and the uterine subluminal stroma surrounding the implanting blastocyst on Day 5 of pregnancy. No corresponding signal was detected in the uterus on Day 5 of pseudopregnancy. Most notably, once delayed implantation was terminated by oestrogen treatment and the embryo implanted, a high level of LDM expression was induced in the subluminal stroma surrounding the implanting blastocyst, whereas no corresponding signal was detected in the delayed implantation uterus. A high level of LDM expression was observed in the uterus decidua on Days 6–8 of pregnancy. Furthermore, LDM expression was induced in the uterine stroma under artificial decidualisation. Oestrogen, but not progesterone, treatment induced a high level of LDM expression in the uterus of ovariectomised mice. These results indicate that LDM is closely related to mouse embryo implantation and can be upregulated by oestrogen.
Additional keywords: decidualisation, implantation.
Acknowledgements
The authors thank Dr M. R. Waterman for supplying the CYP51 antibody. This work was supported by grants from the Chinese 973 Project (No. 2006CB504003 and 2007CB947401) and the National Natural Science Foundation of China (No. 30470868, 30571358 and 30671508). The authors declare that there is no conflict of interest that would prejudice the impartiality of this work.
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