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RESEARCH ARTICLE
Contents Vol 22(8)

Identification of apolipoprotein C-I in rainbow trout seminal plasma

Joanna Nynca A B , Mariola A. Dietrich A , Halina Karol A and Andrzej Ciereszko A
+ Author Affiliations
- Author Affiliations

A Department of Gamete and Embryo Biology, Semen Biology Group, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland.

B Corresponding author. Email: nynec@pan.olsztyn.pl

Reproduction, Fertility and Development 22(8) 1183-1187 https://doi.org/10.1071/RD10066
Submitted: 30 March 2010  Accepted: 11 May 2010   Published: 1 October 2010

Abstract

Three distinct bands with high electrophoretic migration rates were isolated and purified from rainbow trout seminal plasma. The molecular masses of these bands were determined to be 5158.8, 4065.9 and 4929.0 Da. The N-terminal amino acids sequences were elucidated and were found to have high homology with Atlantic salmon apolipoprotein C-I. It can be concluded that apolipoprotein C-I is a major component of rainbow trout seminal plasma. Further studies are necessary to confirm the protective effects of apolipoprotein C-I on spermatozoa in terms of the stabilisation of the sperm membrane.

Additional keywords: amino acid sequence, ApoC-I, fish, lipoproteins, milt, molecular mass, proteins, purification.


Acknowledgement

This work was supported by funds appropriated to the Institute of Animal Reproduction and Food Research.


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