Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
RESEARCH ARTICLE

Effects of recipient mouse strain, sex and gonadal status on the outcome of testis tissue xenografting1

Sepideh Abbasi A and Ali Honaramooz A B
+ Author Affiliations
- Author Affiliations

A Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon,Saskatchewan S7N 5B4, Canada.

B Corresponding author. Email: ali.honaramooz@usask.ca

Reproduction, Fertility and Development 22(8) 1279-1286 https://doi.org/10.1071/RD10084
Submitted: 25 April 2010  Accepted: 31 May 2010   Published: 1 October 2010

Abstract

The aim of the present study was to examine factors that may affect the outcome of testis tissue xenografting. Recipient factors were examined by grafting small fragments of testis tissue from newborn piglets under the back skin of immunodeficient mice of different strains (severe combined immunodeficiency (SCID) v. nude), sex (male v. female) and gonadal status (intact v. gonadectomised) using a factorial design (eight groups; n = 7 mice per group). Recipient mice were killed after 8 months to compare the gross and histological attributes of the recovered grafts. Overall, approximately 94% of grafts were recovered. Gonadectomy of male or female recipients did not affect any of the measured outcomes of testis tissue xenografting, therefore data were pooled. Overall, in terms of sex, male mice and, in terms of strain, SCID mice tended to show higher gross and histological development of grafts. The group of female nude mice had the lowest graft recovery rate (75%) compared with the other groups (95–100%; P < 0.05). The grafts from male SCID mice were, on average the largest and had the highest percentage of spermatozoa-containing seminiferous tubules among all the groups (P < 0.05). These results suggest that male SCID mice provide a suitable recipient model for testis tissue xenografting and that the mice do not need to be castrated for optimal results.

Additional keywords: immunodeficient mice, pig, severe combined immunodeficiency (SCID).


Acknowledgements

The authors thank Brian Andries and his staff, especially Margot Meiklejohn, at the Prairie Swine Center, M. Burmester, P. Mason and their staff for animal care, Drs G. Adams, A. Barth and R. Mapletoft for critical review of the manuscript and the University of Saskatchewan Western College of Veterinary Medicine for scholarships to S.A. This study was supported by grants from the Saskatchewan Health Research Foundation (SHRF) and the Natural Sciences and Engineering Research Council (NSERC) of Canada to A.H.


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1 Portions of this study were presented at the 36th Annual Meeting of the International Embryo Transfer Society.


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