Ontogenesis of phase I hepatic drug metabolic enzymes in sheep
Manoja Pretheeban A , Geoff Hammond A , Stelvio Bandiera B , Wayne Riggs B and Dan Rurak A C
+ Author Affiliations
- Author Affiliations
A Department of Obstetrics and Gynecology, Child and Family Research Institute, Faculty of Medicine, University of British Columbia, V5Z 4H4, Canada.
B Faculty of Pharmaceutical Sciences, University of British Columbia, V6T 1Z3, Canada.
C Corresponding author. Email: drurak@cw.bc.ca
Reproduction, Fertility and Development 24(3) 425-437 https://doi.org/10.1071/RD11159
Submitted: 16 June 2011 Accepted: 5 August 2011 Published: 4 November 2011
Abstract
Cytochrome P450 (CYP) enzymes are important for the metabolism of many drugs. While there is information on their identity and ontogeny in humans and rodents, similar data in sheep are lacking. In the present study, cDNA sequences of several CYP enzymes (CYP2A6, CYP2C19, CYP2D6) were cloned by rapid amplification of cDNA ends. In adult, newborn and fetal sheep the mRNA and protein levels of these CYPs and the regulatory factor, hepatic nuclear factor 4α (HNF4α) were determined in liver samples using real-time PCR and western blotting. The effect of antenatal glucocorticoid on these enzymes was also studied by i.v. infusion of cortisol (0.45 mg h–1; 80 h) to another group of fetuses. The mRNA and protein levels of the CYPs and HNF4α were low or absent in the fetus, followed by increasing levels in the newborn and adult. Fetal cortisol administration significantly increased the mRNA and protein levels of CYP2D6. Moreover, the correlation observed between the CYP and HNF4α mRNA levels suggests a possible regulatory role for this transcription factor. The findings suggest that fetal and newborn lambs have a low ability to metabolise drugs that are substrates of these enzymes, and that this ability increases with advancing postnatal age, similar to the situation in humans.
Additional keywords: cortisol, cytochrome P450, hepatocyte nuclear factor 4α, mRNA expression, RT-PCR, sheep liver, western blot.
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