Shotgun proteomics of rainbow trout ovarian fluid
Joanna Nynca A C , Georg J. Arnold B , Thomas Fröhlich B and Andrzej Ciereszko A
+ Author Affiliations
- Author Affiliations
A Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland.
B Laboratory for Functional Genome Analysis (LAFUGA), Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität, Feodor Lynen Str. 25, 81377 Munich, Germany.
C Corresponding author. Email: j.nynca@pan.olsztyn.pl
Reproduction, Fertility and Development 27(3) 504-512 https://doi.org/10.1071/RD13224
Submitted: 17 July 2013 Accepted: 18 December 2013 Published: 30 January 2014
Abstract
In the present study we used a shotgun proteomic approach to identify 54 proteins of rainbow trout ovarian fluid. The study has unravelled the identity of several proteins not previously reported in fish ovarian fluid. The proteome of trout ovarian fluid consists of diverse proteins participating in lipid binding and metabolism, carbohydrate and ion transport, innate immunity, maturation and ovulation processes. Most trout ovarian fluid proteins correspond to follicular fluid proteins of higher vertebrates, but 15% of the proteins were found to be different, such as those related to the immune system (precerebellin-like protein), proteolysis (myeloid cell lineage chitinase), carbohydrate and lipid binding and metabolism (vitellogenins), cell structure and shape (vitelline envelope protein gamma) and a protein with unknown functions (UPF0762 protein C6orf58 homologue). The present study could help in the decoding of the biological function of these proteins and in the discovery of potential biomarkers of oocyte quality.
Additional keywords: immunity, mass spectrometry, ovarian fluid, rainbow trout, shotgun proteomics.
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