Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
RESEARCH ARTICLE

Morphometric developmental pattern of bovine handmade cloned concepti in late pregnancy

R. P. C. Gerger A F , F. C. Zago B , E. S. Ribeiro A I , S. Gaudencio Neto A C , L. T. Martins A C , L. H. Aguiar A C , V. H. V. Rodrigues C , F. H. Furlan A H , I. Ortigari Jr. A , R. D. Sainz D , C. L. Ferrell E J , M. A. Miglino F , C. E. Ambrósio F , J. L. Rodrigues G , R. Rossetto C , F. Forell A , L. R. Bertolini C and M. Bertolini A C G K
+ Author Affiliations
- Author Affiliations

A Center of Agronomy and Veterinary Sciences, Santa Catarina State University (CAV/UDESC), Av. Luiz de Camões, 2090, Bairro Conta Dinheiro, Lages, SC 88.520-000, Brazil.

B Santa Catarina State Agriculture Research and Extension Center (EPAGRI), Rua João José Godinho, S/N, PO Box 181, Bairro Morro do Posto, Lages, SC 88.502-970, Brazil.

C Molecular and Developmental Biology Laboratory, University of Fortaleza (UNIFOR), Av. Washington Soares, n.1321, Bairro Edson Queiroz. Fortaleza, CE 60.811-905, Brazil.

D Department of Animal Science, University of California, Davis (UC Davis), CA 95616-8521, USA.

E US Meat Animal Research Center, ARS, USDA, Clay Center, NE 68933, USA.

F School of Veterinary Medicine and Animal Science, University of Sao Paulo (USP), Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo, SP 05.508-270, Brazil.

G School of Veterinary Medicine (FAVET), Federal University of Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9090, Bairro Agronomia, Porto Alegre, RS 91540-000, Brazil.

H Present address: School of Agronomy and Veterinary Medicine, Federal University of Mato Grosso (UFMT), Cuiabá, MT, 78060-900, Brazil.

I Present address: Department of Animal Biosciences, University of Guelph, 50 Stone Road East, Guelph N1L 2W1, Canada.

J Present address: Ferrell Farms, 1631 Road 316, Saronville, NE 68975, USA.

K Corresponding author. Email: marcelo.bertolini@ufrgs.br

Reproduction, Fertility and Development 29(5) 950-967 https://doi.org/10.1071/RD15215
Submitted: 30 May 2015  Accepted: 15 January 2016   Published: 9 March 2016

Abstract

Cloning procedures often interfere with conceptus growth and life ex utero, in a set of symptoms known as abnormal offspring syndrome (AOS). The aim of the present study was to compare the developmental pattern of in vivo-derived (IVD), IVF-derived and handmade cloning-derived (NT-HMC) Day 225 bovine concepti using established procedures. Pregnancy diagnosis was performed on Day 30 following blastocyst transfer on Day 7. Conceptus morphometry was assessed by ultrasonography on Day 51, and on Day 225 pregnant cows were killed for morphological examination of concepti. Pregnancy outcome was similar between groups, with greater pregnancy losses in the first trimester (70.6%) and smaller fetuses on Day 51 in the NT-HMC group than in the IVD (14.3%) and IVF (20.0%) groups. However, NT-HMC-derived concepti were twofold larger on Day 225 of gestation than controls. A higher frequency (63.5%) of placentomes larger than the largest in the IVD group was observed in the NT-HMC group, which may be relevant to placental function. Conceptus traits in the IVF group were similar to the IVD controls, with only slight changes in placentome types. Morphological changes in cloned concepti likely affected placental function and metabolism, disrupting the placental constraining mechanism on fetal growth in mid- to late pregnancy.

Additional keywords: abnormal offspring syndrome, cattle, conceptus development, embryo transfer, IVF, nuclear transfer, placenta.


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