Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
RESEARCH ARTICLE

GRIM-19, a gene associated with retinoid-interferon-induced mortality, affects endometrial receptivity and embryo implantation

Yang Yang A * , Yanyan Sun A * , Laiyang Cheng A , Anna Li A , Yanjun Shen A , Ligang Jiang A , Xiaohui Deng A and Lan Chao A B
+ Author Affiliations
- Author Affiliations

A Infertility Center, Qilu Hospital, Shandong University, 107 West Wenhua Road, Jinan, Shandong, 250012, China.

B Corresponding author. Email: qlszcl@163.com

Reproduction, Fertility and Development 29(7) 1447-1455 https://doi.org/10.1071/RD16104
Submitted: 2 March 2016  Accepted: 7 June 2016   Published: 27 June 2016

Abstract

GRIM-19 is associated with apoptosis, abnormal proliferation, immune tolerance and malignant transformation, and it also plays an important role in early embryonic development. Although the homologous deletion of GRIM-19 causes embryonic lethality in mice, the precise role of GRIM-19 in embryo implantation has not been elucidated. Here we show that GRIM-19 plays an important role in endometrial receptivity and embryo implantation. Day 1 to Day 6 pregnant mouse uteri were collected. Immunohistochemistry studies revealed the presence of GRIM-19 on the luminal epithelium and glandular epithelium throughout the implantation period in pregnant mice. The protein and mRNA levels of GRIM-19 were markedly decreased on Day 4 of pregnancy in pregnant mice, but there was no change in GRIM-19 levels in a group of pseudopregnant mice. Overexpression of GRIM-19 decreased the adhesion rate of RL95–2–BeWo co-cultured spheroids and increased apoptosis. Furthermore, STAT3 and IL-11 mRNA and protein levels were reduced by overexpressing GRIM-19, but protein and mRNA levels of TNF-α were increased. These findings indicate the involvement of GRIM-19 in the embryo implantation process by regulating adhesion, apoptosis and immune tolerance.

Additional keywords: adhesion, apoptosis, immune tolerance.


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