Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
RESEARCH ARTICLE

Fish oil and wheat-germ oil supplementation restores ovarian function in streptozotocin-diabetic rats

Naglaa F. Khedr

Department of Biochemistry, Faculty of Pharmacy, Tanta University, El-Bahar Street, Medical Compound, Tanta 31527, Egypt. Email: naglaa_khedr2000@yahoo.com

Reproduction, Fertility and Development - https://doi.org/10.1071/RD16135
Submitted: 30 March 2016  Accepted: 18 August 2016   Published online: 28 September 2016

Abstract

Diabetes is a chronic metabolic disorder and has a profound impact on women’s reproductive health. This study aimed to investigate the protective effect of a mixture of fish oil (FO) and wheat-germ oil (WGO) on ovarian dysfunction in diabetic rats. Female Albino rats were divided into control, diabetic and FO–WGO-diabetic groups. Diabetes was induced by intraperitoneal injection of 65 mg kg–1 streptozotocin (STZ). Three weeks later, rats were given oral supplement of 0.4 g kg–1 oil mix (1000 mg FO + 100 mg WGO) daily for 3 weeks. Antioxidant activity was assessed by measuring malondialdehyde (MDA) and reduced glutathione (GSH) levels, the GSH : oxidised glutathione (GSSG) ratio and superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) activities. Ovary function was indicated by serum concentrations of FSH, oestradiol (E2), LH, anti-Müllerian hormone (AMH), ovary histopathology and follicle counts. Anti-inflammatory properties were detected by measuring nuclear factor (NF)-κB in follicular cells by immunohistochemistry. FO–WGO supplementation enhanced CAT, SOD and GPx activities and raised GSH levels and the GSH : GSSG ratio. Supplementation also increased FSH, E2, LH and AMH levels and follicle counts. Moreover, NF-kB expression and MDA were reduced. These findings indicate that FO–WGO supplementation preserved ovarian function in STZ-induced diabetic rats.

Additional keywords: diabetes, follicle-stimulating hormone, ovary, oxidative stress.


References

Agarwal, A., Gupta, S., and Sharma, R. K. (2005). Role of oxidative stress in female reproduction. Reprod. Biol. Endocrinol. 3, 28.
Role of oxidative stress in female reproduction.CrossRef | 16018814PubMed | open url image1

Akman, L., Erbas, O., Akdemir, A., Yavasoglu, A., Taskiran, D., and Kazandi, M. (2015). Levetiracetam ameliorates ovarian function in streptozotocin-induced diabetic rats. Gynecol. Endocrinol. 31, 657–662.
| 1:CAS:528:DC%2BC28Xht1yltbk%3D&md5=8eb315392d67d55499116da91418f5e1CAS | 26291800PubMed | open url image1

Akoh, C. C., and Min, D. B. (2008). ‘Food Lipids: Chemistry, Nutrition, and Biotechnology’. 3rd edn. (CRC Press, Taylor and Francis Group: New York.)

Al-Safi, Z. A., Liu, H., Carlson, N. E., Chosich, J., Harris, M., Bradford, A. P., Robledo, C., Eckel, R. H., and Polotsky, A. J. (2016). Omega-3 fatty acid supplementation lowers serum FSH in normal weight but not obese women. J. Clin. Endocrinol. Metab. 101, 324–333.
Omega-3 fatty acid supplementation lowers serum FSH in normal weight but not obese women.CrossRef | 1:CAS:528:DC%2BC28XjtlSntb8%3D&md5=0c8ce85c034a3bbb4a8bcf14d227c44dCAS | 26523525PubMed | open url image1

Allain, C. C., Poon, L. S., Chan, C. S. G., Richmond, W., Paul, C., and Fu, P. C. (1974). Enzymatic determination of total serum cholesterol. Clin. Chem. 20, 470–475.
| 1:CAS:528:DyaE2cXkt1arsL8%3D&md5=4b86c361c9c8b4cc2bfa5c0c6e54355dCAS | 4818200PubMed | open url image1

Ballester, J., Muñoz, M. C., Domínguez, J., Palomo, M. J., Rivera, M., Rigau, T., Guinovart, J. J., and Rodríguez-Gil, J. E. (2007). Tungstate administration improves the sexual and reproductive function in female rats with streptozotocin-induced diabetes. Hum. Reprod. 22, 2128–2135.
Tungstate administration improves the sexual and reproductive function in female rats with streptozotocin-induced diabetes.CrossRef | 1:CAS:528:DC%2BD2sXos12hurg%3D&md5=f89a99b5b0155aad75358ffc1745293aCAS | 17588954PubMed | open url image1

Broughton, K. S., Bayes, J., and Culver, B. (2010). High α-linolenic acid and fish oil ingestion promotes ovulation to the same extent in rats. Nutr. Res. 30, 731–738.
High α-linolenic acid and fish oil ingestion promotes ovulation to the same extent in rats.CrossRef | 1:CAS:528:DC%2BC3cXhtl2gt7fL&md5=402c6a72880476646322d427292c165eCAS | 21056289PubMed | open url image1

Castaño, G., Arruzazabala, M. L., Fernández, L., Mas, R., Carbajal, D., Molina, V., Illnait, J., Mendoza, S., Gámez, R., Mesa, M., and Fernández, J. (2006). Effects of combination treatment with policosanol and omega-3 fatty acids on platelet aggregation: a randomized, double-blind clinical study. Curr. Ther. Res. Clin. Exp. 67, 174–192.
Effects of combination treatment with policosanol and omega-3 fatty acids on platelet aggregation: a randomized, double-blind clinical study.CrossRef | 24678094PubMed | open url image1

Codner, E., Merino, P. M., and Tena-Sempere, M. (2012). Female reproduction and Type 1 diabetes: from mechanisms to clinical findings. Hum. Reprod. Update 18, 568–585.
Female reproduction and Type 1 diabetes: from mechanisms to clinical findings.CrossRef | 1:CAS:528:DC%2BC38XhtFClu7vO&md5=578e15df40e29daba364d99c787614d8CAS | 22709979PubMed | open url image1

Dasilva, G., Pazos, M., García-Egido, E., Gallardo, J. M., Rodríguez, I., Cela, R., and Medina, I. (2015). Healthy effect of different proportions of marine ω-3 PUFAs EPA and DHA supplementation in Wistar rats: lipidomic biomarkers of oxidative stress and inflammation. J. Nutr. Biochem. 26, 1385–1392.
Healthy effect of different proportions of marine ω-3 PUFAs EPA and DHA supplementation in Wistar rats: lipidomic biomarkers of oxidative stress and inflammation.CrossRef | 1:CAS:528:DC%2BC2MXhslGks73L&md5=8cba3f2bb2c7af2660688c707777727fCAS | 26320676PubMed | open url image1

Ekstrand-Hammarström, B., Osterlund, C., Lilliehöök, B., and Bucht, A. (2007). Vitamin E down-modulates mitogen-activated protein kinases, nuclear factor-kappa B and inflammatory responses in lung epithelial cells. Clin. Exp. Immunol. 147, 359–369.
Vitamin E down-modulates mitogen-activated protein kinases, nuclear factor-kappa B and inflammatory responses in lung epithelial cells.CrossRef | 17223979PubMed | open url image1

Erbas, O., Pala, H. G., Pala, E. E., Oltulu, F., Aktug, H., Yavasoglu, A., and Taskiran, D. (2014). Ovarian failure in diabetic rat model: nuclear factor-kappa B, oxidative stress, and pentraxin-3. Taiwan J. Obstet. Gynecol. 53, 498–503.
Ovarian failure in diabetic rat model: nuclear factor-kappa B, oxidative stress, and pentraxin-3.CrossRef | 25510691PubMed | open url image1

Erbas, O., Pala, H. G., Pala, E. E., Artunc Ulkumen, B., Akman, L., Akman, T., Oltulu, F., Aktug, H., and Yavasoglu, A. (2015). Therapeutic effect of sunitinib on diabetes mellitus related ovarian injury: an experimental rat model study. Gynecol. Endocrinol. 31, 388–391.
Therapeutic effect of sunitinib on diabetes mellitus related ovarian injury: an experimental rat model study.CrossRef | 1:CAS:528:DC%2BC2MXhtVaju7vL&md5=5beae3d80eeb2795a76df2e5ea215fecCAS | 25703256PubMed | open url image1

Flachs, P., Rossmeisl, M., and Kopecky, J. (2014). The effect of n-3 fatty acids on glucose homeostasis and insulin sensitivity. Physiol. Res. 63, S93–S118.
| 1:CAS:528:DC%2BC2cXhvVSrurvN&md5=7891f420eb34cc973a8556deca38a3c0CAS | 24564669PubMed | open url image1

Ghadge, A., Harsulkar, A., Karandikar, M., Pandit, V., and Kuvalekar, A. (2016). Comparative anti-inflammatory and lipid normalizing effects of metformin and omega-3 fatty acids through modulation of transcription factors in diabetic rats. Genes Nutr. 11, 10.
Comparative anti-inflammatory and lipid normalizing effects of metformin and omega-3 fatty acids through modulation of transcription factors in diabetic rats.CrossRef | 27551311PubMed | open url image1

Geethan, P. K., and Prince, P. S. (2008). Antihyperlipidemic effect of D-Pinitol on streptozotocin-induced diabetic Wistar rats. J. Biochem. Mol. Toxicol. 22, 220–224.
Antihyperlipidemic effect of D-Pinitol on streptozotocin-induced diabetic Wistar rats.CrossRef | 18752266PubMed | open url image1

Hughes, J., Kwong, W. Y., Li, D., Salter, A. M., Lea, R. G., and Sinclair, K. D. (2011). Effects of omega-3 and-6 polyunsaturated fatty acids on ovine follicular cell steroidogenesis, embryo development and molecular markers of fatty acid metabolism. Reproduction 141, 105–118.
Effects of omega-3 and-6 polyunsaturated fatty acids on ovine follicular cell steroidogenesis, embryo development and molecular markers of fatty acid metabolism.CrossRef | 1:CAS:528:DC%2BC3MXisVehs7k%3D&md5=9d6a93b5a4108a6d6cdcc36bee8d0c91CAS | 21045166PubMed | open url image1

Hussein, J. S., El-Khayat, Z., Morsy, S., Oraby, F., and Gamal Singer, G. (2014). The effect of fish oil on oxidant/antioxidant status in diabetic rats through the reduction of arachidonic acid in the cell membrane. Int. J. Pharm. Sci. 6, 196–199. open url image1

Karim, B. O., Landolfi, J. A., Christian, A., Ricart-Arbona, R., Qiu, W., McAlonis, M., Eyabi, P. O., Khan, K. A., Dicello, J. F., Mann, J. F., and Huso, D. L. (2003). Estrous cycle and ovarian changes in a rat mammary carcinogenesis model after irradiation, tamoxifen chemoprevention, and aging. Comp. Med. 53, 532–538.
| 1:CAS:528:DC%2BD3sXptFOjt7c%3D&md5=75ca7e27db78f21d81cb64e8d5e673a7CAS | 14655997PubMed | open url image1

Khedr, N. F. (2015). Protective effect of mirtazapine and hesperidin on cyclophosphamide-induced oxidative damage and infertility in rat ovaries. Exp. Biol. Med. (Maywood) 240, 1682–1689.
Protective effect of mirtazapine and hesperidin on cyclophosphamide-induced oxidative damage and infertility in rat ovaries.CrossRef | 1:CAS:528:DC%2BC2MXitVWhs7fI&md5=872c3eb6bc4d8fbe29b5ff3a4c8c45dcCAS | 25787947PubMed | open url image1

Khowailed, A., Mohammad, O., Elattar, S., and Gaber, S. (2012). Effect of sildenafil on gonadotrophin and sex steroids in fructose induced diabetes in female rats. Med. J. Cairo Univ. 80, 243–252. open url image1

Kim, N. N., Stankovic, M., Cushman, T. T., Goldstein, I., Munarriz, R., and Traish, A. M. (2006). Streptozotocin-induced diabetes in the rat is associated with changes in vaginal hemodynamics, morphology and biochemical markers. BMC Physiology 6, 4.
Streptozotocin-induced diabetes in the rat is associated with changes in vaginal hemodynamics, morphology and biochemical markers.CrossRef | 16734901PubMed | open url image1

Klotzsch, S. G., and McNamara, J. R. (1990). Triglyceride measurements: a review of methods and interferences. Clin. Chem. 36, 1605–1613.
| 1:CAS:528:DyaK3cXmtVOltrY%3D&md5=bd83cba45576435e0baf6a8d911c5316CAS | 2208701PubMed | open url image1

Kumar, P., and Sait, S. F. (2011). Luteinizing hormone and its dilemma in ovulation induction. J. Hum. Reprod. Sci. 4, 1–7.
Luteinizing hormone and its dilemma in ovulation induction.CrossRef | 1:CAS:528:DC%2BC3MXkt1KrurY%3D&md5=6ba495b8f22b1797335ed875420341f6CAS | open url image1

Landau, S., and Everitt, B. S. (2004) ‘Handbook of Statistical Analyses Using SPSS’. (Chapman & Hall–CRC Press LLC: Florida)

Leenhardt, F., Fardet, A., Lyan, B., Gueux, E., Rock, E., Mazur, A., Chanliaud, E., Demigné, C., and Rémésy, C. (2008). Wheat germ supplementation of a low vitamin E diet in rats affords effective antioxidant protection in tissues. J. Am. Coll. Nutr. 27, 222–228.
Wheat germ supplementation of a low vitamin E diet in rats affords effective antioxidant protection in tissues.CrossRef | 1:CAS:528:DC%2BD1cXhtV2htLvO&md5=d8ba43b21280ee732c72fc56b50f4c05CAS | 18689553PubMed | open url image1

Lowry, O. H., Rosenbrough, N. J., Farr, A. L., and Randall, R. J. (1951). Protein measurement with the folin– phenol reagent. J. Biol. Chem. 193, 265–275.
| 1:CAS:528:DyaG38XhsVyrsw%3D%3D&md5=8ecdf487a304856e8a089ea361fb46cbCAS | 14907713PubMed | open url image1

Maeda, K. I., Kura, S. O., and Tsukamura, H. (2000) Physiology of reproduction in the laboratory rat. In ‘Handbook of Experimental Animals’. (Ed. C. J. Hrinke.) pp. 145–176. (Academic Press: London.)

Megahad, O. A., and El Kinawy, O. S. (2002). Studies on the extraction of wheat germ oil by commercial hexane. Grasas y Aceites 53, 414–418.
Studies on the extraction of wheat germ oil by commercial hexane.CrossRef | 1:CAS:528:DC%2BD3sXjsVarsLg%3D&md5=9086c1e42acaf4f3b48171774d008e1aCAS | open url image1

Mehranjani, M. S., Abnosi, M. H., Naderi, A., and Mahmodi, M. (2007). Preventing effects of wheat germ oil on sex hormones, liver enzymes, lipids and proteins in rat serum following treatment with p-nonylphenol. J. Biol. Sci. 7, 1406–1411.
Preventing effects of wheat germ oil on sex hormones, liver enzymes, lipids and proteins in rat serum following treatment with p-nonylphenol.CrossRef | 1:CAS:528:DC%2BD1cXntlajtQ%3D%3D&md5=009ff87eb08617dd79ebcba81f795d1eCAS | open url image1

Mehranjani, M. S., Noorafshan, A., Hamta, A., Momeni, H. R., Abnosi, M. H., Mahmoodi, M., Anvari, M., and Hazaveh, M. (2010). Effects of vitamin E on ovarian tissue of rats following treatment with p-nonylphenol: a stereological study. Iranian J. Reprod. Med. 8, 1–9.
| 1:CAS:528:DC%2BC3cXosFeiu70%3D&md5=7952fa2ce814a69282d353f290d45599CAS | open url image1

Merghani, B. H., Awadin, W. F., Elseady, Y. Y., and Abu-Heakal, S. A. N. (2015). Protective role of wheat germ oil against hyperglycemia and hyperlipidemia in streptozotocin induced diabetic rats. Asian J. Anim. Vet. Adv. 10, 852–864.
Protective role of wheat germ oil against hyperglycemia and hyperlipidemia in streptozotocin induced diabetic rats.CrossRef | open url image1

Misra, H. P., and Fridovich, I. (1972). The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J. Biol. Chem. 247, 3170–3175.
| 1:CAS:528:DyaE38XhsFSntLk%3D&md5=22cb6775e11cec395139dd1a13bc777fCAS | 4623845PubMed | open url image1

Ohashi, K., Ishikawa, H., and Ohta, Y. (2011). Octacosanol ameliorates hyperlipidemia and oxidative stress in KKAy mice with Type 2 diabetes. J. Anal. Bio-Sci. 34, 223–233.
| 1:CAS:528:DC%2BC3MXhtFyisLnK&md5=81d8987707a0ee36272adf64fbf5e366CAS | open url image1

Ohkawa, H., Ohishi, N., and Yagi, K. (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95, 351–358.
Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction.CrossRef | 1:CAS:528:DyaE1MXksFaisbk%3D&md5=1f0e420aeb8b481d1a03911a2d947932CAS | open url image1

Okewumi, T. A., Edoh, E. G., Shittu, S. T., Oyeyemi, F. B., Ugwuezumba, P. C., and Oyeyemi, W. A. (2012). Vitamin E improves growth rate and reproductive functions in female rats exposed to nicotine. Int. J. Biol. Med. Res. 3, 1715–1719. open url image1

Ouladsahebmadarek, E., Khaki, A., Khanahmadi, S., Ashtiani, H. A., Paknejad, P., and Ayubi, M. R. (2014). Hormonal and metabolic effects of polyunsaturated fatty acid (omega-3) on polycystic ovary syndrome induced rats under diet. Iran. J. Basic Med. Sci. 17, 123–127.
| 24711896PubMed | open url image1

Paglia, D. E., and Valentine, W. N. (1967). Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J. Lab. Clin. Med. 70, 158–169.
| 1:CAS:528:DyaF2sXks1Wjur8%3D&md5=e9a21e976d763becb560e3e5b1aed0b4CAS | 6066618PubMed | open url image1

Patrelli, T. S., Gizzo, S., Sianesi, N., Levati, L., Pezzuto, A., Ferrari, B., and Bacchi Modena, A. (2012). Anti-Müllerian hormone serum values and ovarian reserve: can it predict a decrease in fertility after ovarian stimulation by ART cycles? PLoS One 7, e44571.
Anti-Müllerian hormone serum values and ovarian reserve: can it predict a decrease in fertility after ovarian stimulation by ART cycles?CrossRef | 1:CAS:528:DC%2BC38Xhtl2jtr7L&md5=e5ff610ecd0f78fdd4556beebee6f9a4CAS | 22984527PubMed | open url image1

Pine, L., Hoffman, P. S., Malcolm, G. B., Benson, R. F., and Keen, M. G. (1984). Determination of catalase, peroxidase, and superoxide dismutase within the genus Legionella. J. Clin. Microbiol. 20, 421–429.
| 1:CAS:528:DyaL2cXlsVOnsbk%3D&md5=b1ded103cb1cf11a4117ed71b2599d2cCAS | 6490828PubMed | open url image1

Pitocco, D., Tesauro, M., Alessandro, R., Ghirlanda, G., and Cardillo, C. (2013). Oxidative stress in diabetes: implications for vascular and other complications. Int. J. Mol. Sci. 14, 21525–21550.
Oxidative stress in diabetes: implications for vascular and other complications.CrossRef | 24177571PubMed | open url image1

Punsawad, C., Maneerat, Y., Chaisri, U., Nantavisai, K., and Viriyavejakul, P. (2013). Nuclear factor kappa B modulates apoptosis in the brain endothelial cells and intravascular leukocytes of fatal cerebral malaria. Malar. J. 12, 260.
Nuclear factor kappa B modulates apoptosis in the brain endothelial cells and intravascular leukocytes of fatal cerebral malaria.CrossRef | 1:CAS:528:DC%2BC3sXht1ynsrbN&md5=3487fbdc167fef67f785a8c84567eb22CAS | 23890318PubMed | open url image1

Robinson, J. G., Ijioma, N., and Harris, W. (2010). Omega-3 fatty acids and cognitive function in women. Womens Health (Lond. Engl.) 6, 119–134.
Omega-3 fatty acids and cognitive function in women.CrossRef | 1:CAS:528:DC%2BD1MXhsFKgtbbN&md5=70f1c6513a8e1845c0f2fd633d366b25CAS | open url image1

Rodríguez, M. D., Sánchez, M., and García, H. (1997). Multigeneration reproduction study of policosanol in rats. Toxicol. Lett. 90, 97–106.
Multigeneration reproduction study of policosanol in rats.CrossRef | 9067477PubMed | open url image1

Soto, N., Iñiguez, G., López, P., Larenas, G., Mujica, V., Rey, R. A., and Codner, E. (2009). Anti-Müllerian hormone and inhibin B concentrations as markers of premature ovarian aging and transition to menopause in Type 1 diabetes mellitus. Hum. Reprod. 24, 2838–2844.
Anti-Müllerian hormone and inhibin B concentrations as markers of premature ovarian aging and transition to menopause in Type 1 diabetes mellitus.CrossRef | 1:CAS:528:DC%2BD1MXhtlSgsrnF&md5=b54033d3e3a2184e9bbccf680f34e045CAS | 19643804PubMed | open url image1

Stenhouse, E. (2012). Effects of diabetes on women’s reproductive health. Nurs. Stand. 26, 35–40.
Effects of diabetes on women’s reproductive health.CrossRef | 22787969PubMed | open url image1

Traish, A. M., Cushman, T., Hoyt, R., and Kim, N. N. (2009). Diabetes attenuates female genital sexual arousal response via disruption of estrogen action. Korean J. Urol. 50, 211–223.
Diabetes attenuates female genital sexual arousal response via disruption of estrogen action.CrossRef | open url image1

Wathes, D. C., Abayasekara, D. R., and Aitken, R. J. (2007). Polyunsaturated fatty acids in male and female reproduction. Biol. Reprod. 77, 190–201.
Polyunsaturated fatty acids in male and female reproduction.CrossRef | 1:CAS:528:DC%2BD2sXot1OnsL0%3D&md5=7d29a1dffcffb1f126a48b4ac1353815CAS | 17442851PubMed | open url image1

Westwood, F. R. (2008). The female rat reproductive cycle: a practical histological guide to staging. Toxicol. Pathol. 36, 375–384.
The female rat reproductive cycle: a practical histological guide to staging.CrossRef | 18441260PubMed | open url image1

Williams, J., Odum, J., Lewis, R. W., and Brady, A. M. (1997). The oral administration of polysorbate 80 to the immature female rat does not increase uterine weight. Toxicol. Lett. 91, 19–24.
The oral administration of polysorbate 80 to the immature female rat does not increase uterine weight.CrossRef | 1:CAS:528:DyaK2sXivVyhu7c%3D&md5=6b8f02dbe8e9b1dc2af8682ec9c234d3CAS | 9096282PubMed | open url image1

Wonnacott, K. E., Kwong, W. Y., Hughes, J., Salter, A. M., Lea, R. G., Garnsworthy, P. C., and Sinclair, K. D. (2010). Dietary omega-3 and -6 polyunsaturated fatty acids affect the composition and development of sheep granulosa cells, oocytes and embryos. Reproduction 139, 57–69.
Dietary omega-3 and -6 polyunsaturated fatty acids affect the composition and development of sheep granulosa cells, oocytes and embryos.CrossRef | 1:CAS:528:DC%2BC3cXovVWnug%3D%3D&md5=fa234c057ebeef96af2d8353644da07bCAS | 19789173PubMed | open url image1

Wu, J., and Yan, L. J (2015). Streptozotocin-induced Type 1 diabetes in rodents as a model for studying mitochondrial mechanisms of diabetic ß cell glucotoxicity. Diabetes Metab. Syndr. Obes. 8, 181–188.
Streptozotocin-induced Type 1 diabetes in rodents as a model for studying mitochondrial mechanisms of diabetic ß cell glucotoxicity.CrossRef | 1:CAS:528:DC%2BC28XosFChsLw%3D&md5=24e2153185a679b64238ff31a6d8ba24CAS | 25897251PubMed | open url image1

Yessoufou, A., Nekoua, M. P., Gbankoto, A., Mashalla, Y., and Moutairou, K. (2015). Beneficial effects of omega-3 polyunsaturated fatty acids in gestational diabetes: consequences in macrosomia and adulthood obesity. Journal of Diabetes Research 2015, Article ID 731434.
Beneficial effects of omega-3 polyunsaturated fatty acids in gestational diabetes: consequences in macrosomia and adulthood obesity.CrossRef | open url image1

Yivgi-Ohana, N., Sher, N., Melamed-Book, N., Eimer, S., Koler, M., Manna, P. R., Stocco, D. M., and Orly, J. (2009). Transcription of steroidogenic acute regulatory protein in the rodent ovary and placenta: alternative modes of cyclic adenosine 3′, 5′-monophosphate dependent and independent regulation. Endocrinology 150, 977–989.
Transcription of steroidogenic acute regulatory protein in the rodent ovary and placenta: alternative modes of cyclic adenosine 3′, 5′-monophosphate dependent and independent regulation.CrossRef | 1:CAS:528:DC%2BD1MXhs1Sgtrk%3D&md5=f68a58b45377f9a92c5a2fe56e1dcf5aCAS | 18845640PubMed | open url image1

Young, L. R., Kurzer, M. S., Thomas, W., Redmon, J. B., and Raatz, S. K. (2011). Effect of dietary fat and omega-3 fatty acids on urinary eicosanoids and sex hormone concentrations in postmenopausal women: a randomized controlled feeding trial. Nutr. Cancer 63, 930–939.
Effect of dietary fat and omega-3 fatty acids on urinary eicosanoids and sex hormone concentrations in postmenopausal women: a randomized controlled feeding trial.CrossRef | 1:CAS:528:DC%2BC3MXhtVWrs73L&md5=08fa1469c015cf3b3b677bc9c9391f0eCAS | 21745038PubMed | open url image1

Zugno, A. I., Chipindo, H., Canever, L., Budni, J., Alves de Castro, A., Bittencourt de Oliveira, M., Heylmann, A. S., Gomes Wessler, P., da Rosa Silveira, F., Damázio, L. S., Mastella, G. A., Kist, L. W., Bogo, M. R., Quevedo, J., and Gama, C. S. (2015). Omega-3 fatty acids prevent the ketamine-induced increase in acetylcholinesterase activity in an animal model of schizophrenia. Life Sci. 121, 65–69.
Omega-3 fatty acids prevent the ketamine-induced increase in acetylcholinesterase activity in an animal model of schizophrenia.CrossRef | 1:CAS:528:DC%2BC2MXhsl2gsQ%3D%3D&md5=a26ff4c2de231e7369356c4218a7b6c4CAS | 25498892PubMed | open url image1



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