Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
RESEARCH ARTICLE

Gene expression profile in heat-shocked Holstein and Nelore oocytes and cumulus cells

J. S. Ticianelli A , I. P. Emanuelli A , R. A. Satrapa A , A. C. S. Castilho A , B. Loureiro A , M. J. Sudano B , P. K. Fontes A , R. F. P. Pinto A , E. M. Razza A , R. S. Surjus C , R. Sartori C , M. E. O. A. Assumpção D , J. A. Visintin D , C. M. Barros A and F. F. Paula-Lopes A E F
+ Author Affiliations
- Author Affiliations

A Department of Pharmacology, Institute of Biosciences, São Paulo State University (UNESP), 18618-970 Botucatu, Brazil.

B Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP), Prof. Dr. Walter Mauricio Correra Street, 18618-970 Botucatu, Brazil.

C Department of Animal Sciences, Luiz de Queiroz College of Agriculture (ESALQ), University of São Paulo (USP), Pádua Dias Avenue 11, 13418-900 Piracicaba, Brazil.

D Department of Animal Reproduction, School of Veterinary Medicine and Animal Science (FMVZ), University of São Paulo (USP), Orlando Marques de Paiva Street, 87, 05508-270 São Paulo, Brazil.

E Institute of Environmental Sciences, Chemistry and Pharmacology, Federal University of São Paulo (UNIFESP), Prof. Artur Riedel Street 275, 09913-030 Diadema, Brazil.

F Corresponding author. Email: paula.lopes29@unifesp.br

Reproduction, Fertility and Development 29(9) 1787-1802 https://doi.org/10.1071/RD16154
Submitted: 15 April 2016  Accepted: 20 September 2016   Published: 2 November 2016

Abstract

The present study determined the transcriptome profile in Nelore and Holstein oocytes subjected to heat shock during IVM and the mRNA abundance of selected candidate genes in Nelore and Holstein heat-shocked oocytes and cumulus cells (CC). Holstein and Nelore cows were subjected to in vivo follicle aspiration. Cumulus–oocyte complexes were assigned to control (38.5°C, 22 h) or heat shock (41°C for 12 h, followed by 38.5°C for 10 h) treatment during IVM. Denuded oocytes were subjected to bovine microarray analysis. Transcriptome analysis demonstrated 127, nine and six genes were differentially expressed between breed, temperature and the breed × temperature interaction respectively. Selected differentially expressed genes were evaluated by real-time polymerase chain reaction in oocytes and respective CC. The molecular motor kinesin family member 3A (KIF3A) was upregulated in Holstein oocytes, whereas the pro-apoptotic gene death-associated protein (DAP) and the membrane trafficking gene DENN/MADD domain containing 3 (DENND3) were downregulated in Holstein oocytes. Nelore CC showed increased transcript abundance for tight junction claudin 11 (CLDN11), whereas Holstein CC showed increased transcript abundance for antioxidant metallothionein 1E (MT1E) . Moreover, heat shock downregulated antioxidant MT1E mRNA expression in CC. In conclusion, oocyte transcriptome analysis indicated a strong difference between breeds involving organisation and cell death. In CC, both breed and temperature affected mRNA abundance, involving cellular organisation and oxidative stress.

Additional keywords: Bos taurus indicus, Bos taurus taurus, mRNA, microarray, real-time polymerase chain reaction.


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