Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
RESEARCH ARTICLE

Low doses of bisphenol A can impair postnatal testicular development directly, without affecting hormonal or oxidative stress levels

Fernanda M. Ogo A B , Glaucia E. M. L. Siervo A B , Géssica D. Gonçalves A B , Rubens Cecchini B , Flavia A. Guarnier B , Janete Ap. Anselmo-Franci C and Glaura S. A. Fernandes A D
+ Author Affiliations
- Author Affiliations

A Department of General Biology, Biological Sciences Center, State University of Londrina, Rodovia Celso Garcia Cid, PR 445 – Km 380, Campus Universitário, 86057-970, Londrina, Paraná, Brazil.

B Department of General Pathology, Biological Sciences Center, State University of Londrina, Rodovia Celso Garcia Cid, PR 445 – Km 380, Campus Universitário, 86057-970, Londrina, Paraná, Brazil.

C Department of Morphology, Physiology and Basic Pathology, School of Dentistry of Ribeirão Preto, University of São Paulo, FORP, Av. do Café – S/N, 14040-904, Ribeirão Preto, São Paulo, Brazil.

D Corresponding author. Email: glaura@uel.br

Reproduction, Fertility and Development 29(11) 2245-2254 https://doi.org/10.1071/RD16432
Submitted: 4 November 2016  Accepted: 22 February 2017   Published: 7 April 2017

Abstract

Bisphenol A (BPA) is considered a potent endocrine disruptor, causing changes in the endocrine system due to its oestrogenic activity. Male individuals may be susceptible to endocrine, morphological and physiological alterations during testicular postnatal development. The aim of the present study was to evaluate whether exposure to BPA during the peripubertal period can damage testicular development. To this end, male Wistar rats were treated with BPA via gavage at doses of 20 or 200 µg kg−1 on Postnatal Days (PND) 36–66. The control group was treated with Oil + DMSO under the same conditions. On PND 67, rats were killed. The blood was collected for hormonal analysis, the testis for sperm count, oxidative stress, histopathological and immunohistochemical analyses for ki-67 and sperm of the vas deferens for morphological analysis. Both doses of BPA resulted in abnormal sperm morphology and seminiferous tubules, with the highest dose increasing the height of the germinal epithelium and reducing the number of spermatozoa at Stages IX–XIII of spermatogenesis. In conclusion, both doses of BPA administered during the peripubertal period impaired testicular development without any effects on hormone levels (luteinizing hormone (LH), follicle stimulating hormone (FSH) and testosterone levels) or oxidative stress.

Additional keywords: puberty, rat, sex hormones, spermatozoa, testis.


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