Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
RESEARCH ARTICLE

Differential effects of high and low glucose concentrations during lipolysis-like conditions on bovine in vitro oocyte quality, metabolism and subsequent embryo development

J. De Bie A D , W. F. A. Marei A B , V. Maillo C , L. Jordaens A , A. Gutierrez-Adan C , P. E. J. Bols A and J. L. M. R. Leroy A

A Gamete Research Centre, Laboratory for Veterinary Physiology and Biochemistry, Department of Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium.

B Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza square, 12211 Giza, Egypt.

C Departamento de Reproduccion Animal, Instituto nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA), Crta. De la Coruña, km 7,5 28040 Madrid, Spain.

D Corresponding author. Email: jessie.debie@uantwerpen.be

Reproduction, Fertility and Development - https://doi.org/10.1071/RD16474
Submitted: 25 November 2016  Accepted: 6 March 2017   Published online: 10 April 2017

Abstract

Lipolytic metabolic conditions are traditionally associated with elevated non-esterified fatty acid (NEFA) concentrations, but may also be accompanied by hyperglycaemia in obesity or by hypoglycaemia during a negative energy balance status. Elevated NEFA concentrations disrupt oocyte and embryo development and quality, but little is known about whether the effects of lipolytic conditions on oocyte developmental competence are modulated by glucose availability. To answer this, bovine cumulus–oocyte complexes (COCs) were matured under different conditions: physiological NEFA (72 µM) and normal glucose (5.5 mM), pathophysiologically high NEFA (420 µM) and normal glucose, high NEFA and high glucose (9.9 mM), high NEFA and low glucose (2.8 mM). Developmental potential, cumulus expansion and metabolism of COCs exposed to high NEFA and low glucose were affected to a greater extent compared with COCs matured under high NEFA and high glucose conditions. High NEFA and high glucose conditions caused a moderate increase in oocyte reactive oxygen species compared with their high NEFA and low glucose or control counterparts. Blastocyst metabolism and the transcriptome of metabolic and oxidative stress-related genes were not affected. However, both lipolytic conditions associated with hyper- or hypoglycaemia led to surviving embryos of reduced quality with regards to apoptosis and blastomere allocation.

Additional keywords: energy balance, fatty acid, fertility, insulin, in vitro maturation, oxidative stress.


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