Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
RESEARCH ARTICLE (Open Access)

Endo-siRNA deficiency results in oocyte maturation failure and apoptosis in porcine oocytes

Wanxin Liu A * , Qi Zhao A * , Shanhua Piao A , Chunsheng Wang A , Qingran Kong B C and Tiezhu An A C
+ Author Affiliations
- Author Affiliations

A Laboratory of Animal Developmental Biology, College of Life Science, Northeast Forestry University, Harbin, Heilongjiang Province 150040, China.

B Laboratory of Embryo Biotechnology, College of Life Science, Northeast Agricultural University, Harbin, Heilongjiang Province 150030, China.

C Corresponding authors. Emails: kqr721726@163.com; antiezhu@qq.com

Reproduction, Fertility and Development 29(11) 2168-2174 https://doi.org/10.1071/RD16498
Submitted: 29 August 2016  Accepted: 31 January 2017   Published: 12 April 2017

Journal Compilation © CSIRO 2017 Open Access CC BY-NC-ND

Abstract

Both microRNAs (miRNAs) and endogenous small interfering RNAs (endo-siRNAs) play key regulatory roles in gene expression. Some studies have demonstrated that the function of miRNA is suppressed in mouse oocytes, suggesting that endo-siRNA, not miRNA, is essential for female meiosis. This finding has yet to be confirmed in other species. In this study, by knockdown of DICER1, DROSHA and its cofactor DiGeorge syndrome critical region 8 (DGCR8) in porcine oocytes, we found that the proportion of oocytes with DICER1 deficiency that developed to meiosis II (MII) stage was significantly lower than oocytes with DROSHA and DGCR8 deficiency (39.23 versus 68.71 and 71.25% respectively; P < 0.05). Oocytes lacking DROSHA and DGCR8 formed a barrel-shaped metaphase I spindle, with chromosomes tightly aligned at the metaphase plate whereas most oocytes (87%) lacking DICER1 showed spindle abnormalities during oocyte in vitro maturation. Furthermore, DICER1 deficiency also resulted in oocyte apoptosis. These results indicate that endo-siRNAs are essential for oocyte maturation in pigs.

Additional keywords: gene regulation, in vitro maturation, livestock.


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