Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
RESEARCH ARTICLE

Genome-wide DNA methylation profile of prepubertal porcine testis

Xi Chen A * , Liu-Hong Shen A * , Li-Xuan Gui B * , Fang Yang A , Jie Li A , Sui-Zhong Cao A , Zhi-Cai Zuo A , Xiao-Ping Ma A , Jun-Liang Deng A , Zhi-Hua Ren A , Zhong-Xu Chen B C and Shu-Min Yu A C
+ Author Affiliations
- Author Affiliations

A College of Veterinary Medicine, Sichuan Agricultural University, No. 211 Huimin Road, Wenjiang District, Chengdu, 611130, China.

B OnMath Science and Technology Limited Company, No. 500 Tianfu Road, Chengdu, Sichuan, 611130, China.

C Corresponding authors. Emails: czhongxu@gmail.com; yayushumin@163.com

Reproduction, Fertility and Development - https://doi.org/10.1071/RD17067
Submitted: 21 February 2017  Accepted: 17 June 2017   Published online: 21 July 2017

Abstract

The biological structure and function of the mammalian testis undergo important developmental changes during prepuberty and DNA methylation is dynamically regulated during testis development. In this study, we generated the first genome-wide DNA methylation profile of prepubertal porcine testis using methyl-DNA immunoprecipitation (MeDIP) combined with high-throughput sequencing (MeDIP-seq). Over 190 million high-quality reads were generated, containing 43 642 CpG islands. There was an overall downtrend of methylation during development, which was clear in promoter regions but less so in gene-body regions. We also identified thousands of differentially methylated regions (DMRs) among the three prepubertal time points (1 month, T1; 2 months, T2; 3 months, T3), the majority of which showed decreasing methylation levels over time. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed that many genes in the DMRs were linked with cell proliferation and some important pathways in porcine testis development. Our data suggest that DNA methylation plays an important role in prepubertal development of porcine testis, with an obvious downtrend of methylation levels from T1 to T3. Overall, our study provides a foundation for future studies and gives new insights into mammalian testis development.

Additional keywords: development, epigenetics, MeDIP-seq, reproduction.


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