Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
RESEARCH ARTICLE

In vivo and in vitro strategies to support caprine preantral follicle development after ovarian tissue vitrification

N. J. Donfack A , K. A. Alves A , B. G. Alves A , R. M. P. Rocha A , J. B. Bruno A , L. F. Lima A , C. H. Lobo A , R. R. Santos B , S. F. S. Domingues B , M. Bertolini C D , J. Smitz E and A. P. R. Rodrigues A F
+ Author Affiliations
- Author Affiliations

A Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Av. Paranjana, 1700, Campus do Itaperi. Fortaleza, CEP: 60740 903 – CE – Brasil.

B Laboratory of Biology and Medicine of Wild Animals of Amazon, Federal University of Pará-Belem, Av. Universitária, CEP: 68745001 – Castanhal, PA – Brasil.

C Federal University of Rio Grande do Sul, Veterinay Faculty, Avenida Bento Gonçalves, 9090, CEP: 91540000 – Porto Alegre, RS – Brasil.

D Laboratory of Molecular Biology and development, Fortaleza University, Edson Queiroz, 60811905 – Fortaleza, CE – Brasil

E Follicle Biology Laboratory, Center for Reproductive Medicine, UZ Brussel, Laarbeeklaan 101, B-1090 Brussels, Belgium.

F Corresponding author. Email: aprrodriguespapers@gmail.com

Reproduction, Fertility and Development - https://doi.org/10.1071/RD17315
Submitted: 2 August 2017  Accepted: 1 December 2017   Published online: 15 January 2018

Abstract

The aim of the present study was to compare fresh and vitrified goat ovarian tissue after autotransplantation and in vitro culture. Adult goats were completely ovariectomised and each ovarian pair was sliced and distributed among six different treatment groups: fresh control, fresh transplant, fresh culture, vitrified control, vitrified transplant and vitrified culture. Follicular morphology, development, growth, density, revascularisation and hormone production were evaluated in all groups. Three antral follicles (two in the fresh transplant and one in the vitrified transplant groups) were observed on the surface of the graft 90 days after transplantation. The percentage of morphologically normal follicles was similar in the fresh control, fresh transplant and vitrified transplant groups. The percentage of developing (transition, primary and secondary) follicles was higher after in vitro culture of fresh or vitrified tissue. Transplantation resulted in a lower follicle density. Serum oestradiol concentrations remained constant during the entire transplantation period. In contrast, progesterone production decreased significantly. Expression of CD31 mRNA was lower in fresh culture. In conclusion, restoration of goat ovarian function can be successfully achieved following transplantation of both fresh and vitrified goat ovarian tissue. However, transplantation induced higher follicle loss than in vitro culture.

Additional keywords: cryopreservation, folliculogenesis, goat, heterotopic transplantation.


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