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Vertebrate reproductive science and technology
RESEARCH ARTICLE

70 Effect of Ovulation Stimulus on Biochemical Composition of the Oviduct of Alpaca (Vicugna pacos)

F. Y. Hilari A , J. C. Villanueva A , W. F. Huanca A , B. Lira B and W. Huanca A
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- Author Affiliations

A Laboratory of Animal Reproduction, Faculty of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru;

B Laboratory of Physiology, Faculty of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru

Reproduction, Fertility and Development 30(1) 173-174 https://doi.org/10.1071/RDv30n1Ab70
Published: 4 December 2017

Abstract

Experiments were designed to determine the effect of ovulation stimulus on the biochemical composition of oviduct fluid in alpacas (Vicugna pacos). Twenty-four adult females were used to determine profiles of protein, albumin, glucose, cholesterol, and triglycerides in oviduct fluid. The animals were stimulated with 1 mL of seminal plasma (SP), 0.042 mg of a gonadotropin-releasing hormone (GnRH) analogue (buserelin acetate; Conceptal, Intervet, Holland), or PBS as control group. The oviduct was removed inmediately after slaughter on Day 2 or 4 after ovulation induction and washed with 3 mL of PBS solution. Determination of the biochemical composition was carried out with specific kits from the FAR Diagnostics Italia laboratory using a semiautomatic biochemical analyzer of the Sinowa China brand, which is based on spectrophotometric evaluation. The results are presented in Table 1. Our results suggest that the method of induction of ovulation affected the biochemical composition of oviduct fluid, including concentrations of albumin, cholesterol, total protein, triglyceride, and glucose.


Table 1.  Biochemical composition of oviduct fluid (mean ± SEM).
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