218. Expression of Wsb2 in the mouse testis
M. Sarraj A B C , P. J. McClive B , K. L. Loveland A C and A. H. Sinclair B CA MIMR, Monash University, Clayton, VIC, Australia
B Murdoch Children’s Research Institute, Melbourne University, Melbourne, VIC, Australia
C The ARC Centre of Excellence in Biotechnology and Development, Australia
Reproduction, Fertility and Development 17(9) 84-84 https://doi.org/10.1071/SRB05Abs218
Submitted: 26 July 2005 Accepted: 26 July 2005 Published: 5 September 2005
Abstract
We present a detailed study on the expression pattern of Wsb2 in the mouse foetal and adult gonad. Wsb2 expression was analysed during mouse embryogenesis by whole-mount, section in situ hybridisation and immunohistochemistry. Wsb2 was found to be expressed in the developing mouse gonads from 11.5 dpc to 16.5 dpc. Expression is initially equal in both sexes from 10.5 dpc until 12.0 dpc, then it persists in the male gonad. Wsb2 expression was confined to the cords in both Sertoli cell and germ cells. Other sites of Wsb2 embryonic expression were the somites, dorsal root ganglia and the lateral mantle layer of the neural tube.
mRNA encoding Wsb2 and Wsb2 protein has been detected in the newborn testis in both gonocytes and Sertoli cells. Wsb2 mRNA in the adult mouse testis was observed in Sertoli cells, spermatogonia, spermatocytes and the corresponding Wsb2 protein expression was in pachytene spermatocytes, round and elongated spermatids, Sertoli cells and Leydig cells.
The differential expression of Wsb2 in male versus female embryonic gonads suggests it may play a role in mammalian sex determination during embryonic development and its expression in the first wave of spermatogenesis and in the adult suggests a later role in spermatogenesis.
