Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology


H. Kohram A , G. Mohammadi B and E. Dirandeh B
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A University of Tehran, Karaj, Tehran, Iran;

B Shahid Chamran University, Ahvaz, Khoozestan, Iran

Reproduction, Fertility and Development 22(1) 379-380
Published: 8 December 2009


This study was done to consider relationships of luteal phase variables (prior to AI) with follicular waves. The estrous cycles of 15 buffaloes were synchronized with 2 i.m. injections of prostaglandin F given 11 days apart. The buffaloes were randomly assigned to 1 of 3 treatments. Buffaloes in the control treatment received no treatment, whereas G6 buffalos received a GnRH injection between Day 5 and 7 and G16 buffalos received a GnRH injection between Day 15 and 17 of the estrous cycle (estrus = Day 0). Daily, from estrus Day 0 to the next estrus Day 23, buffaloes had their ovaries scanned by ultrasound. Blood samples were collected by tail following each ultrasound examination from estrus until next estrus (estrus = 0). Concentrations of plasma progesterone were determined by radioimmunoassay kit. The limit of detection of the assay was 0.1 45 ng mL-1 and the intra- and interassay coeffients of variation were 7.4% and 9.2%, respectively. Data were analyzed by using PROC GLM of SAS (SAS Institute, Cary, NC, USA). For comparisons between groups, the 2-sample t-test was used for continuous traits, such as size of CL or hormone concentrations. Prospective comparisons of indices of progesterone indicated that the length of luteal lifespan was longer in 3-wave than in 2-wave buffaloes (P < 0.01). Plasma progesterone concentrations were similar at peak and measured as area under the curve on Day 5 through 17 preceding insemination in 2-wave (5.30 ± 0.40 ng mL-1) and 3-wave buffaloes (5.10 ± 0.20 ng mL-1). Length of the luteal phase (defined as from the day of estrus until the last day on which plasma progesterone remained >2 ng mL-1) was <2 days shorter in 2-wave buffaloes than in 3-wave buffaloes (15.20 ± 0.40 v. 17.10 ± 0.50 d; P < 0.05). In addition, the day of peak progesterone occurred earlier in 2-wave buffaloes (13.50 ± 0.30 v. 15.30 ± 0.70 d; P < 0.05).

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