16 SPERM DISTRIBUTION AFTER LAPAROSCOPIC INTRAUTERINE INSEMINATION OF FROZEN GOAT SEMEN

N. Anakkul; J. Suwimonteerabutr; T. Tharasanit; S. Khunmanee; P. Diloksumpan; D. K. Berg; M. Techakumphu

doi:10.1071/RDv26n1Ab16

RESEARCH ARTICLE

16 SPERM DISTRIBUTION AFTER LAPAROSCOPIC INTRAUTERINE INSEMINATION OF FROZEN GOAT SEMEN

N. Anakkul ^A , J. Suwimonteerabutr ^A , T. Tharasanit ^A , S. Khunmanee ^B , P. Diloksumpan ^B , D. K. Berg ^C and M. Techakumphu ^A

+ Author Affiliations

- Author Affiliations

^A Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand;

^B The Office of the Commission on Agricultural Resource Education, Chulalongkorn University, Nan, Thailand;

^C AgResearch, Ltd., Ruakura Research Center, Hamilton, New Zealand

Reproduction, Fertility and Development 26(1) 122-122 https://doi.org/10.1071/RDv26n1Ab16
Published: 5 December 2013

Abstract

Generally, laparoscopic intrauterine insemination (LII) provides a higher success rate than that of cervical insemination in goat. However, the sperm distribution following LII in goat remains unknown, especially when frozen semen is used. This study aimed to evaluate the distribution of frozen-thawed goat sperm after LII. In experiment 1, the frozen-thawed sperm were stained with CellTracker^TM Green CMFDA (CT-Green) or CellTrackerTM Red CMPTX (CT-Red), and then evaluated in vitro for viability and motility at 0, 3, 6, and 9 h after staining. In experiment 2, the CT-Green and CT-Red labelled sperm (30 × 10⁶ sperm in 0.125 mL in each side) were laparoscopicaly inseminated into the left and right uterine horns, respectively (n = 4). After ovariohysterectomy at 6 h post-insemination, the distributions of green and red coloured sperm were assessed by tissue section and sperm flushing. The comparison of semen parameters among groups (control, CT-Green, and CT-Red) were statistically analysed by a general linear model. The results revealed that the fluorochromes used in this study did not impair the sperm motility and viability (P > 0.05). The frozen-thawed goat sperm transuterine-migrated following LII, as both CT-Green (left insemination) and CT-Red labelled sperm (right insemination) were found in both sides of the reproductive tracts. This study concludes that LII in goat would be simpler and consume less time if the semen was unilaterally deposited.

Supported by a grant from Chulalongkorn University Centenary Academic Development Project and RGJ-PhD-industrial link program, Thailand Research Fund (PHD/0156/2550).