Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology

Just Accepted

This article has been peer reviewed and accepted for publication. It is in production and has not been edited, so may differ from the final published form.

Differential oviductal gene expression in porcine estrous cycle

Omar Salvador Acuña , Manuel Aviles , Rebeca Lopez-Ubeda , Ascensión Guillén Martinez , Cristina Soriano Ubeda , A Torrecillas , Pilar Coy , María José Izquierdo-Rico

Abstract

The oviduct undergoes changes under the influence of steroid hormones during the estrous cycle. However, the molecular mechanisms underlying oviductal regulation are not fully understood. The aim of this study was to identify the gene expression profile of the porcine oviduct in different stages of the cycle using microarray technology. A systematic study was made from animals in four different situations: prepubertal gilts and sows in preovulatory, postovulatory and luteal phase. The results showed that the porcine oviduct expressed a total of 4929 genes. Moreover, significant differences in the expression of several genes were detected as the estrous cycle progressed. Analysis ofthe differentially expressed genes indicated that a total of 86, 89 and 15 genes were up-regulated in prepubertal gilts, preovulatory and luteal sows, respectively, compared with the levels observed in the postovulatory animals. Moreover, 80, 51 and 64 genes were down-regulated in prepubertal, preovulatory and luteal animals, respectively, compared to the postovulatory sows. The concentrations of 10 selected transcripts were quantified by real time RT-PCR to validate the cDNA array hybridization data. On the other hand, the localization of protein expression in the oviduct was analysed by immunohistochemistry in the case of some genes (CCK, GPX2, MUC1, PEBP4 and TAC3) and mass spectrometric analysis of oviductal fluid allowed identification of peptides from all five proteins. The results of this study demonstrate that gene expression profiling in the porcine oviduct is clearly regulated during the estrous cycle while some oviductal proteins that could be related to several reproductive processes is described for the first time.

RD16457  Accepted 03 April 2017

© CSIRO 2017