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Molecular cloning and epigenetic change detection of Kiss1 during seasonal reproduction in Chinese indigenous sheep
Like most seasonal domesticated species, sheep are short-day breeders, which mean that reproduction axis can be activated by short days. The annual photoperiodic cycle affects the amount of daylength information that is transmitted to the hypothalamic–pituitary–gonadal (HPG) by regulating pulsatile secretion of GnRH from the hypothalamus. Kisspeptin, which is encoded by Kiss1, plays a major role in reproductive seasonality. Based on results from our previous Solexa sequencing data obtained from Tan (T) and Small Tail Han (STH) sheep during anestrus and the breeding season, full-length mRNA information for ovine Kiss1 was obtained; 894 bp in T sheep and 1145 bp in STH sheep; both encode 135 amino acids. Additionally, T and STH sheep have different transcription start sites of Kiss1. Kiss1 expression during estrus was significantly higher than that during the diestrus stage, both in T and STH sheep (p<0.01). We also found a strong relationship between Kiss1 mRNA levels and histone H3 acetylation status in 5′ promoter region of ovine Kiss1. These data indicated that epigenetic modification occurs during reproduction in sheep, and this is the first report that histone H3 deacetylation occurs in the hypothalamus of seasonal sheep breeders during the transition from diestrus to estrus.
RD17028 Accepted 23 September 2017
© CSIRO 2017