Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology

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The Protective Effects of Polydatin on Experimental Testicular Torsion and Detorsion Injury in Rats

Huilian Qiao , He Ma , Wanjun Cao , Hao Chen , Jinhua Wei , Zhen Li


Oxidative stress plays a critical role in the process of testicular torsion/detorsion (T/D). The purpose of this study was to investigate the protective effect of polydatin (PD) on testicular torsion-detorsion (T/D) injury. Rats were randomly divided into three groups: sham, T/D (2 h/24 h), and T/D plus PD (intraperitoneally injected 30 minutes before detorsion). Unilateral orchiectomy was performed after 24h of reperfusion. Half of the testes were prepared for histological examination by hematoxylin-eosin staining and the TUNEL technique. For the remaining tissues, we analyzed the level of malonaldehyde (MDA), catalase (CAT), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD), and the expression of several apoptosis-related proteins. Compared with the T/D group, PD replenishment significantly ameliorated the morphological damages, lowered the Cosentino histologic score, and increased the mean numbers of germ cell layers and Johnsen's testicular biopsy score. Meanwhile, PD treatment remarkably decreased the level of MDA, and up-regulated the activities of CAT, GSH-Px and SOD. Furthermore, PD decreased T/D-induced germ cell-specific apoptosis, attenuated the activation of caspase-3, caspase-8, caspase-9, poly-ADP-ribose polymerase (PARP), and increased the ratio of Bcl-2/Bax. Our findings indicate that PD has a protective effect against testicular T/D injuries, especially at the histological, anti-oxidative stress and anti-apoptotic levels.

RD17046  Accepted 20 March 2017

© CSIRO 2017