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Transcriptome profiling of stallion spermatozoa, potential implications for male fertility
Poor fertility of stallions is a recognized problem. The aim was to identify candidate genes with a potential influence on their fertility using two different fertile groups. Spermatic RNA was isolated and a list of genes with different transcript abundance between the groups (P≤ 0.05, FC≥ 1.2) was established. 437 genes were found to be significantly different abundant in the two groups (p≤ 0.05, FC≥ 1.2). Genes with differentially abundant transcripts were analyzed for enrichment of functional annotation groups as defined in Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway using Database for Annotation, Visualization, and Integrated Discovery (DAVID). The Ingenuity Pathways Analysis (IPA) was used to identify top biological functions and significant canonical pathways associated with the DE genes. Functional annotation chart using DAVID database showed significant enrichment of three genes. Eight genes were found significantly enriched in the KEGG pathway cytokine-cytokine receptor interaction with (P=0.02). Furthermore, IPA analysis showed interconnected biological functions and canonical pathways involved in the regulation of spermatogenesis and male fertility. For the first time this study identified molecular processes in stallion spermatozoa that could be associated with stallion fertility.
RD17188 Accepted 18 July 2017
© CSIRO 2017