This article has been peer reviewed and accepted for publication. It is in production and has not been edited, so may differ from the final published form.
Recent Advances for Spermatogonial Stem Cell Transplantation in Livestock
At the foundation of spermatogenesis is the actions of spermatogonial stem cells (SSCs) and a remarkable feature of these cells is the capacity to regenerate spermatogenesis following transplantation into testes of a recipient male that lacks endogenous germline. This ability could be exploited in livestock production as a breeding tool to enhance genetic gain. A key element to success is derivation of culture conditions that support proliferation of SSCs to provide sufficient numbers of cells for transfer into multiple recipient males. Using methodology devised for rodent cells as a foundation, advancements in culturing cattle SSCs have occurred over the last few years and efforts are underway to extend this capability to pig cells. Another critical component to SSC transplantation is generation of males with germline ablation but intact somatic support cell function that can serve as surrogate sires for donor-derived spermatogenesis in a natural mating scheme. Recent advances in pigs using gene editing technologies have demonstrated that knockout of a key male germ cell specific gene, NANOS2, leads to male specific germline ablation but otherwise normal physiology including intact seminiferous tubules. Together with recent advances in culturing spermatogonia of higher order mammals, the now efficient means of producing germline ablated recipient males have brought application of SSC transplantation in livestock as a production tool closer to reality than ever before.
RD17418 Accepted 12 October 2017
© CSIRO 2017