Chlamydomonas cDNAs

Abstract

The Chlamydomonas genome project involves 1) sequencing cDNAs isolated from cells exposed to a diverse range of environmental conditions, 2) construction of a high density DNA microarray, 3) construction of genomic contigs centered around specific physical and genetic markers, 4) generation of a complete chloroplast genome sequence and analyses of chloroplast gene expression, and 5) placement of the genomic information on the network in a user friendly format. Aspects of the project emphasized by our group at Stanford involve generation of normalized cDNA libraries, sequencing of cDNAs to generate contigs and unigene families and the use of this information to construct high-density cDNA microarrays. I will discuss techniques for generating and analyzing cDNA sequence information, the use of the different cDNA libraries to identify differentially expressed genes (by in silico subtractions) and strategies for constructing high-density cDNA microarrays. Emphasis will be placed on information relating to genes involved in photosynthesis.