Register      Login
Australian Systematic Botany Australian Systematic Botany Society
Taxonomy, biogeography and evolution of plants
RESEARCH ARTICLE

Pseudoheterocaryum, a new genus segregated from Heterocaryum (Boraginaceae) on the basis of molecular data

Nasim Saadati A , Maryam Khoshsokhan Mozaffar B , Mahboubeh Sherafati A and Shahrokh Kazempour Osaloo A C
+ Author Affiliations
- Author Affiliations

A Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, 14115-154 Tehran, Iran.

B Department of Biology, Islamic Azad University, Qom Branch, Qom, Iran.

C Corresponding author. Email: skosaloo@modares.ac.ir

Australian Systematic Botany 30(1) 105-111 https://doi.org/10.1071/SB16022
Submitted: 20 May 2016  Accepted: 10 February 2017   Published: 31 May 2017

Abstract

The phylogeny of Heterocaryum and Suchtelenia was examined using sequence data from the internal transcribed spacer region of the nuclear rDNA (ITS) and plastid trnL intron and trnL–trnF intergenic spacer (trnL–F) regions. Results indicated that Heterocaryum is non-monophyletic because of the inclusion of Suchtelenia calycina (C.A.Mey.) A.DC. Heterocaryum laevigatum (Kar. & Kir.) A.DC. formed a distinct branch sister to S. calycina and remaining Heterocaryum species. Hence, all species of Heterocaryum except H. laevigatum (type species of the genus) are transferred to a new genus, Pseudoheterocaryum. Taxonomic descriptions are presented for Pseudoheterocaryum and Heterocaryum, as well as a diagnostic key to the three genera included in the present study.

Additional keywords: Boraginaceae, cpDNA trnL–trnF, molecular phylogeny, nrDNA ITS, Suchtelenia.


References

Chacón J, Luebert F, Hilger HH, Ovchinnikova S, Selvi F, Cecchi L, Guilliams CM, Hasenstab-Lehman K, Sutorý K, Simpson MG, Weigend M (2016) The borage family (Boraginaceae s.str.). A revised infrafamilial classification based on new phylogenetic evidence, with emphasis on the placement of some enigmatic genera. Taxon 65, 523–546.
The borage family (Boraginaceae s.str.). A revised infrafamilial classification based on new phylogenetic evidence, with emphasis on the placement of some enigmatic genera.Crossref | GoogleScholarGoogle Scholar |

Cohen JI (2014) A phylogenetic analysis of morphological and molecular characters of Boraginaceae: evolutionary relationships, taxonomy, and patterns of character evolution. Cladistics 30, 139–169.
A phylogenetic analysis of morphological and molecular characters of Boraginaceae: evolutionary relationships, taxonomy, and patterns of character evolution.Crossref | GoogleScholarGoogle Scholar |

de Candolle AP (1846) Boragineae. In ‘Prodromus Systematis Naturalis Regni Vegetabilis’, vol. 10, pp. 1–178. (Treuttel & Würtz.: Paris, France)

Doyle JJ, Doyle JS (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19, 11–15.

Edgar RC (2004) Muscle: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32, 1792–1797.
Muscle: multiple sequence alignment with high accuracy and high throughput.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXisF2ks7w%3D&md5=36806c9d5d01abcba9d7ce8ef97d9cb9CAS |

Farris JS, Kallersjo M, Kluge AG, Bult C (1994) Testing significance of incongruence. Cladistics 10, 315–319.
Testing significance of incongruence.Crossref | GoogleScholarGoogle Scholar |

Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.
Confidence limits on phylogenies: an approach using the bootstrap.Crossref | GoogleScholarGoogle Scholar |

Kazempour Osaloo S (1993) Revision of the tribe Eritrichieae (Boraginaceae) in Iran. M.Sc. thesis, Tarbiat Modares University, Tehran.

Kazmi SMA (1970) A revision of the Boraginaceae of West Pakistan and Kashmir. Journal of the Arnold Arboretum 51, 133–184.

Khatamsaz M (2001) Pollen morphology of Iranian Boraginaceae family and its taxonomic significance. Iranian Journal of Botany 9, 27–40.

Khatamsaz M (2002) Boraginaceae. In ‘Flora of Iran’. (Eds M. Assadi, M. Khatamsaz, A.A. Maassoumi) Vol. 39, pp. 3–504. (Research Institute of Forests and Rangelands: Tehran, Iran)

Khoshsokhan M, Kazempour Osaloo S, Saadatmand S, Attr F (2010) Molecular phylogeny of Rochelia (Boraginaceae) based on nrDNA ITS and cpDNA trnL–F sequences. Iranian Journal of Botany 16, 22–29.

Khoshsokhan Mozaffar M, Kazempour Osaloo S, Oskoueyian R, Naderi Saffar K, Amirahmadi A (2013) Tribe Eritrichieae (Boraginaceae s.str.) in west Asia; a molecular phylogenetic perspective. Plant Systematics and Evolution 299, 197–208.
Tribe Eritrichieae (Boraginaceae s.str.) in west Asia; a molecular phylogenetic perspective.Crossref | GoogleScholarGoogle Scholar |

Mabberley DJ (2008) ‘The Plant-Book: a Portable Dictionary of the Higher Plants.’ (Cambridge University Press: New York, NY, USA)

McNeill J, Barrie FR, Buck WR, Demoulin V, Greuter W, Hawksworth DL, Herendeen PS, Knapp S, Marhold K, Prado J, Prud’homme van Reine WF, Smith GF, Wiersema JH, Turland NJ (2012) ‘International Code of Nomenclature for Algae, Fungi, and Plants (Melbourne Code)’, adopted by the Eighteenth International Botanical Congress, July 2011, Melbourne, Australia. Regnum Vegetabile, vol. 154. (Koeltz Scientific Books: Königstein, Germany)

Nazaire M, Hufford L (2012) A broad phylogenetic analysis of Boraginaceae: implications for the relationships of Mertenisa. Systematic Botany 37, 758–783.
A broad phylogenetic analysis of Boraginaceae: implications for the relationships of Mertenisa.Crossref | GoogleScholarGoogle Scholar |

Ovchinnikova SV (2007) The system of the tribe Eritrichieae. Botanicheskii Zhurnal 92, 751–759.

Popov MG (1974) Boraginaceae. In ‘Flora of USSR’. (Ed. BK Shischkin) Vol. 19, pp. 73–508. (Keter Press: Jerusalem. Israel) [Translated from Russian, Israel Program for Scientific Translations].

Posada D, Buckley TR (2004) Model selection and model averaging in phylogenetics: advantages of akaike information criterion and Bayesian approaches over likelihood ratio tests. Systematic Biology 53, 793–808.
Model selection and model averaging in phylogenetics: advantages of akaike information criterion and Bayesian approaches over likelihood ratio tests.Crossref | GoogleScholarGoogle Scholar |

Riedl H (1967) Boraginaceae. In ‘Flora Iranica’. (Ed. KH Rechinger) Vol. 48, pp. 1–281. (Akademische Druck: Graz, Austria)

Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572–1574.
MrBayes 3: Bayesian phylogenetic inference under mixed models.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXntlKms7k%3D&md5=3dbc2d5e642526e674c8ecd165e5af8dCAS |

Sang T, Crawford DJ, Stuessy T (1995) Documentation of reticulate evolution in peonies (Paeonia) using internal transcribed spacer sequences of nuclear ribosomal DNA: implications for biogeography and concerted evolution. Proceedings of the National Academy of Sciences of the United States of America 92, 6813–6817.
Documentation of reticulate evolution in peonies (Paeonia) using internal transcribed spacer sequences of nuclear ribosomal DNA: implications for biogeography and concerted evolution.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXntVSksLw%3D&md5=41b76950de6771d3066b589c2282ebbfCAS |

Silvestro D, Michalak I (2012) raxmlGUI: a graphical front-end for RAxML. Organisms, Diversity & Evolution 12, 335–337.
raxmlGUI: a graphical front-end for RAxML.Crossref | GoogleScholarGoogle Scholar |

Taberlet P, Gielly L, Pautou G, Bouvet J (1991) Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Molecular Biology 17, 1105–1109.
Universal primers for amplification of three non-coding regions of chloroplast DNA.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK38Xhslel&md5=132500aaf69c35a4dcd7c749b4d2ea52CAS |

Weigend M, Gottschling M, Selvi F, Hilger HH (2009) Marbleseeds are gromwells: systematics and evolution of Lithospermum and allies (Boraginaceae tribe Lithospermeae) based on molecular and morphological data. Molecular Phylogenetics and Evolution 52, 755–768.
Marbleseeds are gromwells: systematics and evolution of Lithospermum and allies (Boraginaceae tribe Lithospermeae) based on molecular and morphological data.Crossref | GoogleScholarGoogle Scholar |

Weigend M, Luebert F, Selvi F, Brokamp G, Hilger HH (2013) Multiple origins for hound’s tongues (Cynoglossum L.) and navel seed (Omphalodes Mill.): the phylogeny of the Borage family (Boraginaceae s.str.). Molecular Phylogenetics and Evolution 68, 604–618.
Multiple origins for hound’s tongues (Cynoglossum L.) and navel seed (Omphalodes Mill.): the phylogeny of the Borage family (Boraginaceae s.str.).Crossref | GoogleScholarGoogle Scholar |

Weigend M, Selvi F, Thomas DC, Hilger HH (2016) Boraginaceae. In ‘The Families and Genera of Vascular Plants. Vol. 14. Flowering Plants: Eudicots; Aquifoliales, Boraginales, Bruniales, Dipsacales, Escalloniales, Garryales, Paracryphiales, Solanales (except Convolvulaceae), Icacinaceae, Metteniusaceae, Vahliaceae’. (Eds JW Kadereit, V Bittrich) pp. 41–102. (Springer International Publishing: Cham, Switzerland) 10.1007/978-3-319-28534-4_5 10.1007/978-3-319-28534-4_5

White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In ‘PCR Protocols: a Guide to Methods and Applications’. (Ed. DH Innis) pp. 315–322. (Academic Press: San Diego, CA, USA)

Zwickl D (2006) Genetic algorithm approaches for the phylogenetic analysis of large biological sequence datasets under the maximum likelihood criterion. PhD thesis, University of Texas, Austin, TX, USA.