Australian Systematic Botany Australian Systematic Botany Society
Taxonomy, biogeography and evolution of plants

Just Accepted

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Death cap mushrooms from southern Australia – additions to Amanita (Amanitaceae, Agaricales) section Phalloideae Clade IX

Elaine Davison , Danielle Giustiniano , Frankie Busetti , Genevieve Gates , Katrina Syme


Three similar Amanita spp. are described: Amanita djarilmari E.M.Davison, A. gardneri E.M.Davison from the south west of Western Australia and A. millsii E.M.Davison & G.M.Gates (=A. sp. 10 ZLY-2014 HKAS 77322 in KUN) from Tasmania. All have a white or pale coloured pileus and white universal veil, but differ in the shape of the bulb, spore shape, and structure of the universal veil. All are from subg. Lepidella sect. Phalloideae. Phylogenetic analysis shows that these species cannot be separated on the basis of data derived from nuclear ribosomal internal transcribed spacer (ITS) sequences. They can be separated in a multi-locus phylogeny of the 28S nuclear ribosomal large subunit rRNA (28S) region, RNA polymerase II (rpb2) region, β-tubulin region and translation elongation factor 1-α (ef1-α) region. Amanita djarilmari, A. gardneri, A. millsii, and two other previously described species in sect. Phalloideae from southern Australia (A. eucalypti and A. marmorata), cluster in Clade IX. These, together with other species in this clade, segregate into two lineages, Clade IX A with a white or pale pileus and Clade IX B with a brown pileus. Solvent extraction, followed by liquid chromatography tandem mass spectrometry of A. djarilmari, A. eucalypti, A. gardneri and A. marmorata basidiomes did not detect the highly toxic amatoxins α-amanitin and β-amanitin, but did detect the phallotoxins phallacidin and phalloidin.

SB17032  Accepted 15 September 2017

© CSIRO 2017