Animal Production Science Animal Production Science Society
Food, fibre and pharmaceuticals from animals
RESEARCH ARTICLE

The acute effect of addition of nitrate on in vitro and in vivo methane emission in dairy cows

P. Lund A C , R. Dahl A , H. J. Yang B C , A. L. F. Hellwing A , B. B. Cao B and M. R. Weisbjerg A
+ Author Affiliations
- Author Affiliations

A Department of Animal Science, Aarhus University, AU Foulum, Blichers Allé 20, PO Box 50, 8830 Tjele, Denmark.

B State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, P.R. China.

C Corresponding authors. Email: Peter.Lund@agrsci.dk; and yang_hongjian@sina.com

Animal Production Science 54(9) 1432-1435 https://doi.org/10.1071/AN14339
Submitted: 13 March 2014  Accepted: 6 June 2014   Published: 17 July 2014

Abstract

The effects of a 24-h administration of a ration high in nitrate (20 g/kg DM) on DM intake and enteric gas production in lactating dairy cows as well as the effect of different doses of nitrate on in vitro fermentation were studied. Nitrate reduced in vivo methane (CH4) production by 31%, and CH4 production increased again when nitrate supplementation was stopped. A similar effect was found when relating CH4 to carbon dioxide, and to DM intake. Addition of nitrate was followed by increased hydrogen production, which decreased again when nitrate was stopped. Nitrate addition did not affect in vitro rumen fermentation in terms of DM degradability, pH, ammonia nitrogen, microbial protein and volatile fatty acid production, but it decreased gas production with longer initial delay time before onset of gas production and lower gas production rate. Nitrate added at 7–20 g/kg ration DM significantly decreased net initial (0–12 h) CH4 production by 10–16%, although no further depression was observed afterwards.

Additional keywords: CH4, gas production, mitigation, ruminant, VFA.


References

Alaboudi AR, Jones GA (1985) Effect of acclimation to high nitrate intakes on some rumen fermentation parameters in sheep. Canadian Journal of Animal Science 65, 841–849.
Effect of acclimation to high nitrate intakes on some rumen fermentation parameters in sheep.CrossRef |

France J, Dijkstra J, Dhanoa MS, López S, Bannink A (2000) Estimating the extent of degradation of ruminant feeds from a description of their gas production profiles observed in vitro: derivation of models and other mathematical considerations. The British Journal of Nutrition 83, 143–150.
Estimating the extent of degradation of ruminant feeds from a description of their gas production profiles observed in vitro: derivation of models and other mathematical considerations.CrossRef | 1:CAS:528:DC%2BD3cXhsVymtbY%3D&md5=77fa13fb17be7c8e157b87489e27f763CAS | 10743493PubMed |

García-Martínez R, Ranilla MJ, Tejido ML, Carro MD (2005) Effects of disodium fumarate on in vitro rumen microbial growth, methane production and fermentation of diets differing in their forage:concentrate ratio. The British Journal of Nutrition 94, 71–77.
Effects of disodium fumarate on in vitro rumen microbial growth, methane production and fermentation of diets differing in their forage:concentrate ratio.CrossRef | 16115335PubMed |

Hellwing ALF, Lund P, Weisbjerg MR, Brask M, Hvelplund T (2012) Technical note: Test of a low-cost and animal-friendly system for measuring methane emissions from dairy cows. Journal of Dairy Science 95, 6077–6085.
Technical note: Test of a low-cost and animal-friendly system for measuring methane emissions from dairy cows.CrossRef | 1:CAS:528:DC%2BC38XhsVCns7bN&md5=ed74d0cfae7037ed09c18fcc405a4ce8CAS |

Li L, Davis J, Nolan J, Hegarty R (2012) An initial investigation on rumen fermentation pattern and methane emission of sheep offered diets containing urea or nitrate as the nitrogen source. Animal Production Science 52, 653–658.

Menke KH, Steingass H (1988) Estimation of the energetic feed value obtained by chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development 28, 7–55.

Menke KH, Raab L, Salewski A, Steingass H, Fritz D, Schneider W (1979) The estimation of the digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when they are incubated with rumen liquor in vitro. Journal of Agricultural Science, Cambridge 93, 217–222.
The estimation of the digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when they are incubated with rumen liquor in vitro.CrossRef | 1:CAS:528:DyaE1MXlsFKitbc%3D&md5=24fee39c14dc618256dd838b10464ef0CAS |

Nolan JV, Hegarty RS, Hegarty J, Godwin IR, Woodgate R (2010) Effects of dietary nitrate on fermentation, methane production and digesta kinetics in sheep. Animal Production Science 50, 801–806.
Effects of dietary nitrate on fermentation, methane production and digesta kinetics in sheep.CrossRef | 1:CAS:528:DC%2BC3cXhtVyrtbzP&md5=fec0fdf6d058f63b6cb019493a242d75CAS |

Ørskov ER (1975) Manipulation of rumen fermentation for maximium food utilization. In ‘World review of nutrition and dietetics. Vol. 22’. (Ed. GH Bourne) pp. 152–182. (Karger Medical and Scientific Publishers: Basel, Switzerland)

SAS (1999) ‘Statistical analytical system users guide.’ (Statistical Analysis Institute: Cary, NC)

Ungerfeld EM, Kohn RA (2006) The role of thermodynamics in the control of rumen fermentation. In ‘Ruminant physiology: digestion, metabolism and impact of nutrition on gene expression, immunology and stress’. (Eds K Sejrsen, T Hvelplund, MO Nielsen) pp. 55–85. (Wageningen Academic Publishers: Wageningen, The Netherlands)

van Zijderveld SM, Gerrits WJJ, Dijkstra J, Newbold JR, Hulshof RBA, Perdok HB (2011) Persistency of methane mitigation by dietary nitrate supplementation in dairy cows. Journal of Dairy Science 94, 4028–4038.
Persistency of methane mitigation by dietary nitrate supplementation in dairy cows.CrossRef | 1:CAS:528:DC%2BC3MXpsVylur4%3D&md5=b403e5eac8ed8ca1f5a91dddfa81918fCAS | 21787938PubMed |

Zhang DF, Yang HJ (2011) In vitro ruminal methanogenesis of a hay-rich substrate in response to different combination supplements of nitrocompounds, pyromellitic diimide and, 2-bromoethanesulphonate. Animal Feed Science and Technology 163, 20–32.
In vitro ruminal methanogenesis of a hay-rich substrate in response to different combination supplements of nitrocompounds, pyromellitic diimide and, 2-bromoethanesulphonate.CrossRef | 1:CAS:528:DC%2BC3cXhs1WktLfM&md5=950d0ea1ed0f02fde2bfddb29181228fCAS |



Rent Article (via Deepdyve) Export Citation Cited By (2)