Animal Production Science Animal Production Science Society
Food, fibre and pharmaceuticals from animals

Effects of anti-ethylene treatments on browning and energy metabolism of harvested litchi fruit

H. Qu A , X. Duan A , X. Su A , H. Liu A and Y. Jiang A B

A South China Botanical Garden, Chinese Academy of Sciences, Leyiju Guangzhou 510650, P. R. China.

B Corresponding author. Email:

Australian Journal of Experimental Agriculture 46(8) 1085-1090
Submitted: 11 November 2004  Accepted: 14 September 2005   Published: 5 July 2006


Litchi fruit were treated with 10 mL/L ethylene (C2H4), 0.1% cobalt chloride (CoCl2) and 1 mL/L 1-methylcyclopropene, respectively, packed individually in closed but vented containers, and then stored at ambient temperature (28–33°C). The effects of these treatments on the browning, energy metabolism and disease incidence of the fruit were investigated. Treatment with C2H4 enhanced the activities of polyphenol oxidase and phenylalanine ammonia-lyase. Ethylene also increased the accumulation of ADP and AMP, but decreased energy charge in fruit peel. Cobalt chloride treatment had an inhibitory effect on increases of peroxidase activity, ATP : ADP ratio, energy charge and ATP, ADP and AMP. Browning index was reduced by 8.4 and 10.4% and disease index was reduced by 10.7 and 12.7% in CoCl2 and 1-methylcyclopropene-treated fruit, respectively, suggesting a relationship between energy metabolism, browning and disease index.


Blankenship SM Dole JM 2003 1-Methylcyclopropene: a review. Postharvest Biology and Technology 28 1 25 DOI

Bradford MV 1976 A rapid and sensitive method using the principle of protein dye for the quantitation of microgram quantities of protein. Analytical Biochemistry 72 248 254 DOI

Bunsiri A Ketsa S Paull RE 2003 Phenolic metabolism and lignin synthesis in damaged pericarp of mangosteen fruit after impact. Postharvest Biology and Technology 29 61 71 DOI

Chen F Li YB Chen MD 1986 The production of ethylene in litchi fruit during storage and its control. Acta Horticulturae Sinica 13 152 156

Corbineau F Gay-Mathieu C Vinel D Come D 2002 Decrease in sunflower (Helianthus annuus) seed viability caused by high temperature as related to energy metabolism, membrane damage and lipid composition. Physiologia Plantarum 116 489 496

Dong L Lurie S Zhou H 2002 Effect of 1-methylcyclopropene on ripening of ‘Canino’ apricots and ‘Royal Zee’ plums. Postharvest Biology and Technology 24 135 145 DOI

Duan XW Jiang YM Su XG Liu H Li YB Zhang ZQ Zheng YH Jiang WB 2004 Role of pure oxygen treatment in browning of litchi fruit after harvest. Plant Science 167 665 668 DOI

Jiang YM Fu JR 1999 Biological and physiological changes involved in browning of litchi fruit caused by water loss. Journal of Horticultural Science and Biotechnology 74 43 46

Jiang YM Joyce DC Terry LA 2001 1-Methylcyclopropene treatment effects on intact and fresh-cut apple. Postharvest Biology and Technology 77 19 21

Ju ZG Liu CL Yuan YB Dai HY Yan SP Xu J 1993 Regulation of phenolics synthesis and its effects on fruit quality of Laiyang Chili (pear). Scientific Agriculture Sinica 26 44 48

Ketsa S Atantee S 1998 Phenolics, lignin, peroxidase activity and increased firmness of damaged pericarp of mangosteen fruit after impact. Postharvest Biology and Technology 14 117 124

Knoester M Linthorst HJM Bol JF Van Loon LC 2001 Involvement of ethylene in lesion development and systemic acquired resistance in tobacco during the hypersensitive reaction to tobacco mosaic virus. Physiological and Molecular Plant Pathology 59 45 57 DOI

Kochba J Laree S Spiegel-Roy P 1977 Difference in peroxidase activity and isoenzymes in embryogenic and non-embryogenic ‘shamouti’ orange-ovular callus lines. Plant and Cell Physiology 18 463 467

Kumar GNM Knowles NR 1996 Oxidative stress results in increased sinks for metabolic energy during aging and sprouting of potato seed-tubers. Plant Physiology 112 1301 1313

Lattanzio V 2003 Bioactive polyphenols: their role in quality and storability of fruit and vegetables. Journal of Applied Botany 77 128 146

Lin ZF Li SS Zhang DL Lin GZ Li YB Liu SX Chen MD 1988 The changes of pigments, phenolics contents and activities of polyphenol oxidase and phenylalanine ammonia-lyase in pericarp of postharvest litchi fruit. Acta Botanica Sinica 30 40 45

Özogul F Taylor KDA Quantick PC Özogul Y 2000 A rapid HPLC-determination of ATP – related compounds and its application to herring stored under modified atmosphere. International Journal of Food Science and Technology 35 549 554

Pang XQ Zhang ZQ Duan XW Ji ZL 2001 The effects of ethylene and 1-methylcyclopropene on pericarp browning of postharvest lychee fruit. Journal of South China Agriculture University 22 11 14

Pereira-Netto AB 2001 Effect of inhibitors of ethylene biosynthesis and signal transduction pathway on the multiplication of in vitro-grown Hancornia speciosa. Plant Cell, Tissue and Organ Culture 66 1 7

Qu HX Sun GC Jiang YM Zhu XR 2001 Pathogenesis-related proteins in litchi after inoculation with Peronophythora litchii. Acta Horticulturae 558 439 442

Rawyler A Pavelic D Gianinazzi C Oberson J Braendle R 1999 Membrane lipid integrity relies on a threshold of ATP production rate in potato cell cultures submitted to anoxia. Plant Physiology 120 293 300

Rogiers SY Kumar GNM Knowles NR 1998 Regulation of ethylene production and ripening by saskatoon (Amelanchier alnifolia Nutt.) fruit. Canadian Journal of Botany 76 1743 1754 DOI

Rupasinghe HPV Murr DP Paliyath G Skog L 2000 Inhibitory effect of 1-MCP on ripening and superficial scald development in ‘McIntosh’ and ‘Delicious’ apples. Journal of Horticultural Science and Biotechnology 75 271 276

Saquet AA Streif J Bangerth F 2000 Changes in ATP, ADP and pyridine nucleotide levels related to the incidence of physiological disorders in ‘Conference’ pears and ‘Jonagold’ apples during controlled atmosphere storage. Journal of Horticultural Science and Biotechnology 75 243 249

Saquet AA Streif J Bangerth F 2003 Energy metabolism and membrane lipid alterations in relation to brown heart development in ‘Coference’ pears during delayed controlled atmosphere storage. Postharvest Biology and Technology 30 123 132

Selvarajah S Bauchot AD John P 2001 Internal browning in cold-stored pineapple is supppressed by a postharvest application of 1-methylcyclopropene. Postharvest Biology and Technology 23 167 170 DOI

Sisler EC Serek M 1997 Inhibition of ethylene response in plants at the receptor level, recent developments. Physiologia Plantarum 100 577 582 DOI

Stange RR McDonald RE 1999 A simple and rapid method for determination of lignin in plant tissues – its usefulness in elicitor screening and comparison to the thioglycolic acid method. Postharvest Biology and Technology 15 185 193 DOI

Tan XJ Li YB 1984 The partial purification and properties of polyphenol oxidase from the pericarp of litchi (Litchi chinensis). Acta Phytophysiology Sinica 10 339 345

Underhill SJR Critchley C 1995 Cellular localisation of polyphenol oxidase and peroxidase activity in Litchi chinensis Sonn. pericarp. Australian Journal of Plant Physiology 22 627 632

Uritani I 1999 Biochemistry on postharvest metabolism and deterioration of some tropical tuberous crops. Botanical Bulletin of Academia Sinica 40 177 183

Veltman RH Lenthéric I Van der Plas LHW Peppelenbos HW 2003 Internal browning in pear fruit (Pyrus communis L. cv Conference) may be a result of a limited availability of energy and antioxidants. Postharvest Biology and Technology 28 295 302

Zhang DL Chen F Liu SX Li YB Jiang YM Guo JY Quantick PC Warren PL 1997 Effects of Pro-long coating on changes in colour and enzyme activity of postharvest litchi fruit. Journal of Tropical and Subtropical Botany 5 54 60

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