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RESEARCH ARTICLE
Contents Vol 46(5)

Seed alkaloid concentrations are not affected by agronomic and phosphorus-nutrition treatments that reduce Pleiochaeta setosa Hughes disease on narrow-leafed lupin (Lupinus angustifolius)

P. Gremigni A B E , M. W. Sweetingham A B and W. A. Cowling C D
+ Author Affiliations
- Author Affiliations

A Centre for Legumes in Mediterranean Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.

B Department of Agriculture and Food Western Australia, 3 Baron-Hay Court, South Perth, WA 6151, Australia.

C School of Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.

D Canola Breeders Western Australia Pty Ltd, 15/219 Canning Highway, South Perth, WA 6151, Australia.

E Corresponding author. Email: pgremigni@agric.wa.gov.au

Australian Journal of Experimental Agriculture 46(5) 681-687 https://doi.org/10.1071/EA05078
Submitted: 16 March 2005  Accepted: 13 September 2005   Published: 12 May 2006

Abstract

The severity of brown spot caused by the fungus Pleiochaeta setosa (Kirchn.) Hughes in narrow-leafed lupin (Lupinus angustifolius L.) is reduced by improving phosphorus (P) nutrition and using agronomic treatments that extend crop rotation or increase cereal stubble retention. The aim of this work was to investigate the impact of these treatments on the alkaloid concentrations of the harvested seed of 3 sweet cultivars of L. angustifolius that differed in their susceptibility to this fungal disease: Merrit (susceptible), Tallerack and Myallie (both moderately resistant). Because abiotic and biotic stresses appear to stimulate plant alkaloid biosynthesis, we hypothesised that higher levels of disease pressure may increase alkaloid concentrations in the harvested seed, particularly in the disease-susceptible Merrit.

The main effects of P nutrition, cereal stubble retention, genotype and crop rotation were significant for the severity of brown spot and plant biomass of narrow-leafed lupin in a field trial in Wongan Hills, Western Australia, but were not significant for total alkaloid concentrations or the relative proportions of individual alkaloids of the harvested seed. Seed total alkaloid concentrations were in most cases below the maximum permitted concentration established for lupin seed (200 mg/kg dry matter) and were independent of the severity of brown spot on leaves. The great fluctuations of seed total alkaloid concentrations observed in grower deliveries to bulk handling facilities and in lupin cultivar yield testing trials are unlikely to result from the nutritional and agronomic treatments such as those investigated in this experiment, or the various disease levels that resulted from these treatments.

Additional keywords: brown spot, crop history, phosphorus, stubble mulching.


Acknowledgments

The Grains Research and Development Corporation (GRDC) is acknowledged for funding this project (UWA 168) and the Chemistry Centre (WA) for providing facilities and support for the chemical analyses of plant samples. We are grateful to Dr Jonathan Clements and Dr John Hamblin for their positive comments on this manuscript.


References


Allen DG, Greirson BN, Harris DJ, Spadek ZE (1990) Alkaloid profiles of various lupin species. Chemistry Centre Western Australia, Occasional Report No. 89B28804–55, Perth.

ANZFA (Australian New Zealand Food Authority) (2001) Lupin alkaloids in food. ANZFA, Technical Report Series No.3, Canberra. Available at http://www.foodstandards.gov.au/mediareleasespublications/publications/ (verified 1 March 2006)

Bolland MDA, Sweetingham MW, Jarvis RJ (2001) Effect of Pleiochaeta setosa on field responses of Lupinus angustifolius and L. luteus to applications of phosphorus. Australian Journal of Experimental Agriculture 41, 549–556.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Bradley FH, Oram RN, Malafant KW (2002) Inheritance of partial resistance to the brown spot disease in Lupinus angustifolius L. Australian Journal of Agricultural Research 53, 919–929.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Christiansen JL, Jørnsgard B, Buskov S, Olsen CE (1997) Effect of drought stress on content and composition of seed alkaloids in narrow-leafed lupin, Lupinus angustifolius L. European Journal of Agronomy 7, 307–314.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Cowling WA (1999) Pedigrees and characteristics of narrow-leafed lupin cultivars released in Australia from 1967 to 1998. Agriculture Western Australia, Bulletin 4365, Perth.

Cowling WA, Tarr A (2004) Effect of genotype and environment on seed quality in sweet narrow-leafed lupin (Lupinus angustifolius L.). Australian Journal of Agricultural Research 55, 745–751.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Culvenor CCJ, Petterson DS (1986) Lupin toxins — alkaloids and phomopsins. In ‘Proceedings of the 4th International Lupin Conference’. pp. 188–198. (WA Department of Agriculture: Perth, WA)

Eilert U (1998) Induction of alkaloid biosynthesis and accumulation in plants and in vitro cultures in response to elicitation. In ‘Alkaloids-Biochemistry, ecology and medicinal applications’. (Eds ME Roberts, M Wink) pp. 219–238. (Plenum Press: New York)

Genstat 5 Committee (1993) ‘Genstat 3 Reference Manual.’ (Clarendon Press: Oxford)

Gladstones JS (1970) Lupins as crop plants. Field Crop Abstracts 23, 123–148. open url image1

Gladstones JS, Crosbie GB (1979) Lupin wild types introduced into Western Australia to 1973. Department of Agriculture of Western Australia, Technical Bulletin No. 43. Perth.

Gremigni P (2002) Control of seed alkaloid levels in narrow-leafed lupin (L. angustifolius L.) through management of plant nutrition and agronomic practices. PhD Thesis, The University of Western Australia, Perth.

Gremigni P, Wong MTF, Edwards NK, Harris DJ, Hamblin J (2001) Potassium nutrition effects on seed alkaloid concentrations, yield and mineral content of lupins (L. angustifolius L.). Plant and Soil 234, 131–142.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Gremigni P, Hamblin J, Harris DJ, Cowling WA (2003) The interaction of phosphorus and potassium with seed alkaloid concentrations, yield and mineral content in narrow-leafed lupin (Lupinus angustifolius L.). Plant and Soil 253, 413–427.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Harborne JB, Baxter H (Eds) (1993) ‘Phytochemical dictionary.’ pp. 284–289. (Taylor & Francis: London)

Harris DJ (1994) Quality production and breeding. In ‘Proceedings of the 1st Australian Lupin Technical Symposium’. (Eds M Dracup, J Palta) pp. 98–105. (WA Department of Agriculture: Perth)

Harris DJ, Wilson PE (1988) A rapid manual method of lupin alkaloid analysis. In ‘Proceedings of the 5th International Lupin Conference’. (Ed. T Twardowski) pp. 598–601. (International Lupin Association: Poznan)

Jarvis RJ, Bolland MDA (1990) Placing superphosphate at different depths in the soil changes its effectiveness for wheat and lupin production. Fertilizer Research 22, 97–107.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Jarvis RJ, Bolland MDA (1991) Lupin grain yields and fertilizer effectiveness are increased by banding superphosphate below the seed. Australian Journal of Experimental Agriculture 31, 357–366.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Longnecker N, Brennan R, Robson A (1998) Lupin nutrition. In ‘Lupins as crop plants-biology, production and utilization’. (Eds JS Gladstones, CA Atkins, J Hamblin) pp. 121–148. (CAB International: Cambridge)

Marschner H (1995) Functions of mineral nutrients: macronutrients. In ‘Mineral nutrition of higher plants’. 2nd edn. (Ed. H Marschner) pp. 299–312. (Academic Press: London)

Petterson DS (1998) Composition and food uses of lupins. In ‘Lupins as crop plants-biology, production and utilization’. (Eds JS Gladstones, CA Atkins, J Hamblin) pp. 353–384. (CAB International: Cambridge)

Petterson DS, Harris DJ, Allen DG (1991) Alkaloids. In ‘Toxic Substances in Crop Plants’. (Eds JP D’Mello, CM Duffus, JH Duffus) pp. 148–179. (The Royal Society of Chemistry: Edinburgh)

Petterson DS, Sipsas S, Mackintosh JB (Eds) (1997) ‘The chemical composition and nutritional value of Australian pulses.’ (Grains Research & Development Corporation: Canberra)

Priddis C (1983) Capillary gas chromatography of lupin alkaloids. Journal of Chromatography 261, 95–101.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Schoofs G, Teichmann S, Hartmann T, Wink M (1983) Lysine decarboxylase in plants and its integration in quinolizidine alkaloid biosynthesis. Phytochemistry 22, 65–69.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Scibor-Marchocka A (1970) Comparative studies on the homologous types of bitter and fodder white lupine. Acta Agrobotanica 23, 23–38. open url image1

Stobiecki M, Wojtaszek P, Bednarek P, Gulewicz K (1996) Changes in quinolizidine alkaloids composition in lupin (Lupinus albus L.) seedlings elicited with CuCl2 and cell wall extract from Colletotrichum lindemuthianum. Acta Physiologiae Plantarum 18, 313–319. open url image1

Sweetingham MW (1984) Pleiochaeta setosa – a root pathogen of lupins. Australian Journal of Plant Pathology 13, 21–22.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Sweetingham MW, Jarvis RJ (1993) The impact of crop establishment techniques on lupin fungal diseases. Australian Grain 3, 48–49. open url image1

Sweetingham MW, Loughman R, Porritt SE (1993) Cereal stubble protects lupins from brown spot. Australian Journal of Experimental Agriculture 33, 469–473.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Waller GR, Nowacki EK (Eds) (1978) ‘Alkaloid biology and metabolism in plants.’ (Plenum Press: New York)

Wink M (1984) Chemical defense of Leguminosae. Are quinolizidine alkaloids part of the antimicrobial defense system of lupins? Zeitschrift fur Naturforschung 39c, 548–552. open url image1

Wink M (1987) Quinolizidine alkaloids: biochemistry, metabolism and function in plants and cell suspension cultures. Planta Medica 53, 509–514. open url image1

Wink M (1993) Quinolizidine alkaloids. In ‘Alkaloids and sulphur compounds. Methods in plant biochemistry 8’. (Ed. PG Waterman) pp. 197–239. (Academic Press: London)

Wink M, Hartmann T (1982) Localisation of the enzymes of quinolizidine alkaloid biosynthesis in leaf chloroplasts of Lupinus polyphyllus. Plant Physiology 70, 74–77.
| PubMed |
open url image1

Wink M, Schimmer O (1999) Mode of action of defensive secondary metabolites. In ‘Function of plant secondary metabolites and their exploitation in biotechnology. Annual Plant Reviews, 3’. (Ed. M Wink) pp. 17–133. (Sheffield Academic Press, CRC Press: Sheffield, England)

Wippich C, Wink M (1985) Biological properties of alkaloids. Influence of quinolizidine alkaloids and gramine on the germination and development of powdery mildew, Erysiphe graminis f. sp. hordei. Experientia 41, 1477–1479.
| Crossref | GoogleScholarGoogle Scholar | open url image1