Register      Login
Australian Journal of Botany Australian Journal of Botany Society
Southern hemisphere botanical ecosystems
RESEARCH ARTICLE

Germination of selected Australian native grass species, with potential for minesite rehabilitation

Grus J. Farley A , Sean M. Bellairs B C and Stephen W. Adkins A
+ Author Affiliations
- Author Affiliations

A The University of Queensland, School of Land, Crop and Food Sciences, St Lucia, Qld 4072, Australia.

B Charles Darwin University, School of Environment, Darwin, NT 0909, Australia.

C Corresponding author. Email: sean.bellairs@cdu.edu.au

Australian Journal of Botany 61(4) 283-290 https://doi.org/10.1071/BT12258
Submitted: 8 October 2012  Accepted: 10 April 2013   Published: 16 May 2013

Abstract

Native grasses have become increasingly important in the post-mining landscape where land rehabilitators try to reconstruct vegetation communities similar to those present before land clearing. So as to include native grasses in these communities, there is a requirement to understand their germination biology, because in the past, many grasses have typically been hard to establish in the final community. The present study found that poor germination of 13 native grass species was due to (1) low percentage of seed fill, (2) low seed viability of filled seeds and/or (3) seed dormancy. Eight species had dormancy treatments investigated. Most were found to exhibit at least one form of dormancy that was either located in the hull structures immediately external to the caryopsis (i.e. the lemma, palea and glumes), within the seed coat (i.e. testa and pericarp, tissues that are found inside the hull, but external to the embryo and endosperm) and/or within the embryo. Seven of the grass species tested were found to have a dormancy mechanism present in two or more locations of their dispersal unit. Germination of the selected native grass species could be improved by (1) processing seeds to enrich the percentage of seeds that are filled, (2) testing viability to ensure a high proportion of the seeds are likely to germinate or (3) using methods to help overcome dormancy and promote germination.

Additional keywords: mining, Poaceae, revegetation, seed dormancy, seed germination.


References

Adkins SW, Bellairs SM (1996) Seed dormancy mechanisms in Australian native species. In ‘Proceedings of the second Australian workshop on native seed biology for revegetation’. (Eds SM Bellairs, JM Osborne) pp. 81–91. (Australian Centre for Minesite Rehabilitation Research: Brisbane)

Adkins SW, Bellairs SM, Loch DS (2002) Seed dormancy mechanisms in warm season grass species. Euphytica 126, 13–20.
Seed dormancy mechanisms in warm season grass species.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XlvFyltLY%3D&md5=d99dfb097b18909f558fa13d8152739cCAS |

Baskin CC, Baskin JM (1998) ‘Seeds: ecology, biogeography, and evolution of dormancy and germination.’ (Academic Press: San Diego, CA)

Bell LC (2001) Establishment of native ecosystems after mining – Australian experience across diverse biogeographic zones. Ecological Engineering 17, 179–186.
Establishment of native ecosystems after mining – Australian experience across diverse biogeographic zones.Crossref | GoogleScholarGoogle Scholar |

Bellairs SM, Read TR, Bumstead E, Paddon B, Farley G (1999) Dormancy of Australian native grass species. In ‘Proceedings of the third Australian workshop on native seed biology for revegetation’. (Eds CJ Asher, LC Bell) pp. 145–156. (Australian Centre for Mining Environmental Research: Brisbane)

Benech Arnold RL, Fenner M, Edwards PJ (1992) Changes in dormancy level in Sorghum halepense seeds induced by water stress during seed development. Functional Ecology 6, 596–605.
Changes in dormancy level in Sorghum halepense seeds induced by water stress during seed development.Crossref | GoogleScholarGoogle Scholar |

Bewley JD, Black M (1994) ‘Seeds: physiology of development and germination.’ 2nd edn. (Plenum Press: New York)

Black M, Wareing PF (1959) The role of germination inhibitors and oxygen in the dormancy of light-sensitive seed of Betula spp. Journal of Experimental Botany 10, 134–145.
The role of germination inhibitors and oxygen in the dormancy of light-sensitive seed of Betula spp.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaF3cXitl2juw%3D%3D&md5=c45240bee203b27b178161225cf73b22CAS |

Brown RF (1982) Seed dormancy in Aristida armata. Australian Journal of Botany 30, 67–73.
Seed dormancy in Aristida armata.Crossref | GoogleScholarGoogle Scholar |

Clarke S, French K (2005) Germination response to heat and smoke of 22 Poaceae species from grassy woodlands. Australian Journal of Botany 53, 445–454.
Germination response to heat and smoke of 22 Poaceae species from grassy woodlands.Crossref | GoogleScholarGoogle Scholar |

Cole IA, Johnston WH (2006) Seed production of Australian native grass cultivars: an overview of current information and future research needs. Australian Journal of Experimental Agriculture 46, 361–373.
Seed production of Australian native grass cultivars: an overview of current information and future research needs.Crossref | GoogleScholarGoogle Scholar |

Cresswell CF, Nelson H (1972) The effects of boron on the breaking, and possible control of dormancy of seed of Themeda triandra Forsk. Annals of Botany 36, 771–780.

Davidson PJ, Adkins SW (1997) Germination of Triodia grass seed by plant derived smoke. In ‘Proceedings of the Australian Rangeland Society 10th biennial conference’, 1–4 December 1997, Gatton College, Gatton. (Eds EJ Moll, MJ Page, B Alchin) pp. 29–30. (Australian Rangeland Society: Aldgate, SA)

Dixon K (1996) Seed quality for mine site restoration and revegetation. In ‘Proceedings of the second Australian workshop on native seed biology for revegetation’. (Eds SM Bellairs, JM Osborne) pp. 15–23. (Australian Centre for Minesite Rehabilitation Research: Brisbane)

Dixon KW, Roche S, Pate JS (1995) The promotive effect of smoke derived from burnt native vegetation on seed germination of Western Australian plants. Oecologia 101, 185–192.
The promotive effect of smoke derived from burnt native vegetation on seed germination of Western Australian plants.Crossref | GoogleScholarGoogle Scholar |

Duclos DV, Ray DT, Johnson DJ, Taylor AG (2013) Investigating seed dormancy in switchgrass (Panicum virgatum L.): understanding the physiology and mechanisms of coat-imposed seed dormancy. Industrial Crops and Products 45, 377–387.
Investigating seed dormancy in switchgrass (Panicum virgatum L.): understanding the physiology and mechanisms of coat-imposed seed dormancy.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXisVOgsbY%3D&md5=21e4ec18e7aa18106ac90b6134936238CAS |

Farley G, Adkins S, Dixon K, Bellairs S, Preston C (2000) Identifying dormancy mechanisms of Australian native plant species. In ‘Proceedings of the third Australian workshop on native seed biology for revegetation’. (Eds CJ Asher, LC Bell) pp. 163–165. (Australian Centre for Mining Environmental Research: Brisbane)

Fendall RK, Carter JF (1965) New-seed dormancy of green needlegrass (Stipa viridula Trin.). I. Influence of the lemma and palea on germination, water absorption and oxygen uptake. Crop Science 5, 533–536.
New-seed dormancy of green needlegrass (Stipa viridula Trin.). I. Influence of the lemma and palea on germination, water absorption and oxygen uptake.Crossref | GoogleScholarGoogle Scholar |

Graeber K, Nakabayashi K, Miatton E, Leubner-Metzger G, Soppe WJJ (2012) Molecular mechanisms of seed dormancy. Plant, Cell & Environment 35, 1769–1786.
Molecular mechanisms of seed dormancy.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xht12itr3K&md5=5bec2aeb88b92cf44e42585ce2bafe0bCAS |

Groves RH, Hagon MW, Ramakrishnan PS (1982) Dormancy and germination of seed of eight populations of Themeda australis. Australian Journal of Botany 30, 373–386.
Dormancy and germination of seed of eight populations of Themeda australis.Crossref | GoogleScholarGoogle Scholar |

Gutterman Y (1992) Maternal effects on seeds during development. In ‘The ecology of regeneration in plant communities’. (Ed. M. Fenner) pp. 27–59. (CAB International: Wallingford, UK)

Hagon MW (1976) Germination and dormancy of Themeda australis, Danthonia spp., Stipa bigeniculata and Bothriochloa macra. Australian Journal of Botany 24, 319–327.
Germination and dormancy of Themeda australis, Danthonia spp., Stipa bigeniculata and Bothriochloa macra.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE28Xlslals7s%3D&md5=58e2f544097e5230d4289869a02fe9f8CAS |

Harrington GT, Crocker W (1923) Structure, physical characteristics, and composition of the pericarp and integument of Johnson grass seed in relation to its physiology. Journal of Agricultural Research 23, 193–222.

International Seed Testing Association (1999) International rules for seed testing 1999. Seed Science and Technology 27, 1–333.

Jacobs SWL (1984) Triodia. In ‘Arid Australia’. (Eds HG Cogger, EE Cameron) pp. 131–142. (Australian Museum: Sydney)

Jacobs SWL (1992) Triodia (Triodia, Plectrachne, Symplectrodia and Monodia: Poaceae) in Australia. In ‘Desertified grasslands: their biology and management’. (Ed. GF Chapman) pp. 47–62. (Academic Press: London)

Jacobsen CN (1981) A review of the species of Dichanthium native to Australia with special reference to their occurrence in Queensland. Tropical Grasslands 15, 84–95.

Jurado E, Westoby M (1992) Germination biology of selected central Australian plants. Australian Journal of Ecology 17, 341–348.
Germination biology of selected central Australian plants.Crossref | GoogleScholarGoogle Scholar |

Li M, Han J, Wang Y, Sun J, Haferkamp M (2010) Different seed dormancy levels imposed by tissues covering the caryopsis in zoysiagrass (Zoysia japonica Steud.) Seed Science and Technology 38, 320–331.

Loch D, Adkins S, Hestlehurst M, Paterson M, Bellairs S (2004) Seed formation, development and germination. In ‘Warm season (C4) grasses’. (Eds LE Moser, BL Burson, LE Sollenberger) (American Society of Agronomy: Madison, WI)

Lodge GM, Whalley RDB (1981) Establishment of warm- and cool-season native perennial grasses on the north-west slopes of New South Wales. I. Dormancy and germination. Australian Journal of Botany 29, 111–119.
Establishment of warm- and cool-season native perennial grasses on the north-west slopes of New South Wales. I. Dormancy and germination.Crossref | GoogleScholarGoogle Scholar |

Ma HY, Liang ZW, Liu M, Wang MM, Wang SH (2010) Mechanism of the glumes in inhibiting seed germination of Leymus chinensis (Trin.) Tzvel. (Poaceae) Seed Science and Technology 38, 655–664.

Martin CC (1975) The role of glumes and gibberellic acid in dormancy of Themeda triandra spikelets. Physiologia Plantarum 33, 171–176.
The role of glumes and gibberellic acid in dormancy of Themeda triandra spikelets.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE2MXhtlGrtb0%3D&md5=33a62f71bd81a92cd4bffba6ccf3f6afCAS |

Mott JJ (1974) Mechanisms controlling dormancy in the arid zone grass Aristida contorta. 1. Physiology and mechanisms of dormancy. Australian Journal of Botany 22, 635–645.
Mechanisms controlling dormancy in the arid zone grass Aristida contorta. 1. Physiology and mechanisms of dormancy.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE2MXht1Wrsbg%3D&md5=d7429e8aedeff164acebe763cc126900CAS |

Mott JJ (1978) Dormancy and germination in five native grass species from savannah woodland communities of the Northern Territory. Australian Journal of Botany 26, 621–631.

Naylor JM, Simpson GM (1961) Dormancy studies in seeds of Avena fatua. 2. A gibberellin-sensitive inhibitory mechanism in the embryo. Canadian Journal of Botany 39, 281–295.
Dormancy studies in seeds of Avena fatua. 2. A gibberellin-sensitive inhibitory mechanism in the embryo.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaF3sXktlWlsbg%3D&md5=ebb8973104c8b4ffe234b11207dfcdcdCAS |

Read TR, Bellairs SM (1999) Smoke affects the germination of native grasses of New South Wales. Australian Journal of Botany 47, 563–576.
Smoke affects the germination of native grasses of New South Wales.Crossref | GoogleScholarGoogle Scholar |

Roche S, Dixon KW, Pate JS (1997) Seed ageing and smoke: partner cues in the amelioration of seed dormancy in selected Australian native species. Australian Journal of Botany 45, 783–815.
Seed ageing and smoke: partner cues in the amelioration of seed dormancy in selected Australian native species.Crossref | GoogleScholarGoogle Scholar |

Simpson GM (1990) ‘Seed dormancy in grasses.’ (Cambridge University Press: Cambridge, UK)

Simpson GM, Naylor JM (1962) Dormancy studies in seed of Avena fatua. 3. A relationship between maltase, amylases and gibberellin. Canadian Journal of Botany 40, 1659–1673.
Dormancy studies in seed of Avena fatua. 3. A relationship between maltase, amylases and gibberellin.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaF3sXotlyquw%3D%3D&md5=e5de281e2626e65bce0579032d6dc34fCAS |

Smith RD, Dickie JB, Linington SH, Pritchard HW, Probert RJ (Eds) (2003) ‘Seed conservation: turning science into practice.’ (Royal Botanic Gardens, Kew: London)

Tothill JC (1977) Seed germination studies with Heteropogon contortus. Australian Journal of Ecology 2, 477–484.
Seed germination studies with Heteropogon contortus.Crossref | GoogleScholarGoogle Scholar |

Vose PB (1956) Dormancy of seeds of Phalaris anmdinacea and Phalaris titherosa. Nature 178, 1006–1007.
Dormancy of seeds of Phalaris anmdinacea and Phalaris titherosa.Crossref | GoogleScholarGoogle Scholar |

Wagner M, Pywell RF, Knopp T, Bullock JM, Heard MS (2011) The germination niches of grassland species targeted for restoration: effects of seed pre-treatments. Seed Science Research 21, 117–131.
The germination niches of grassland species targeted for restoration: effects of seed pre-treatments.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXlslags7k%3D&md5=1580cfb18d246589a9e012ce56040207CAS |

Wareing PF, Foda HA (1957) Growth inhibitors and dormancy in Xanthium seed. Physiologia Plantarum 10, 266–280.
Growth inhibitors and dormancy in Xanthium seed.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaG1MXisFGh&md5=3ad1f17d31a92c71a06ec1f1dce7cc4dCAS |

Waters CM, Loch DS, Johnston PW (1997) The role of native grasses and legumes for land revegetation in central and eastern Australia with particular reference to low rainfall areas. Tropical Grasslands 31, 304–310.

Way MJ (2003) Collecting seed from non-domesticated plants for long-term conservation. In ‘Seed conservation: turning science into practice’. (Eds RD Smith, JB Dickie, SH Linington, HW Pritchard, RJ Probert) pp. 165–201. (Royal Botanic Gardens, Kew: London)

Westoby M, Rice M, Griffin G, Friedel M (1988) The soil seed bank of Triodia basedowii in relation to time since fire. Australian Journal of Ecology 13, 161–169.
The soil seed bank of Triodia basedowii in relation to time since fire.Crossref | GoogleScholarGoogle Scholar |

Whalley RDB, Davidson AA (1969) Drought dormancy in Astrebla lappacea, Chloris acicularis, and Stipa aristiglumis. Australian Journal of Agricultural Research 20, 1035–1042.
Drought dormancy in Astrebla lappacea, Chloris acicularis, and Stipa aristiglumis.Crossref | GoogleScholarGoogle Scholar |

Whitehorne GJ, McIntyre DK (1975) A method for breaking seed dormancy in Boronia spp., Eriostemon spp. and other native Australian species. Combined Proceedings of the International Plant Propagation Society 25, 291–294.

Wulff RD (1986) Seed size variation in Desmodium paniculatum. 1. Factors affecting seed size. Journal of Ecology 74, 87–97.
Seed size variation in Desmodium paniculatum. 1. Factors affecting seed size.Crossref | GoogleScholarGoogle Scholar |

Xu Q, Bughrara SS, Nelson CJ, Coutts JH (2005) Mechanisms of seed dormancy in zoysia (Zoysia japonica Steud.). Seed Science and Technology 33, 543–550.