Australian Journal of Botany Australian Journal of Botany Society
Southern hemisphere botanical ecosystems

Factors That Influence the Yield and Viability of Cucumber (Cucumis sativus L) Cotyledon Protoplasts

AS Kantharajah and WA Dodd

Australian Journal of Botany 38(2) 169 - 175
Published: 1990


Protoplasts isolated from cotyledons of aseptically germinated cucumber seedlings were divided into three size classes. The relationships between tissue age, isolation procedure, yield and protoplast size were investigated. During germination and up to an age of 13 days, the percentage of protoplasts in each size class underwent considerable change with a big reduction in percentage of the largest protoplasts in older cotyledons. Protoplast size and yield could also be manipulated by varying the isolation technique. In this context, temperature, incubation time and shaker speed were significant. By selecting tissue of appropriate age and using a carefully selected isolation procedure the percentage of viable cucumber protoplasts with the ability to form a cell wall and divide can be increased considerably.

© CSIRO 1990

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