Australian Journal of Chemistry Australian Journal of Chemistry Society
An international journal for chemical science
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Imaging on the Nanoscale: Super-Resolution Fluorescence Microscopy

Liisa M. Hirvonen A B and Trevor A. Smith A
+ Author Affiliations
- Author Affiliations

A ARC Centre of Excellence for Coherent X-Ray Science, School of Chemistry, University of Melbourne, Parkville, VIC 3010, Australia.

B Corresponding author. Email: liisah@unimelb.edu.au

Australian Journal of Chemistry 64(1) 41-45 https://doi.org/10.1071/CH10333
Submitted: 10 September 2010  Accepted: 14 November 2010   Published: 14 January 2011

Abstract

Although the resolution of a light microscope is fundamentally limited by diffraction to about half of the wavelength of light, in recent years several techniques have been developed that can overcome this limitation in fluorescence microscopy, allowing imaging with nanometre scale resolution. Many of these techniques are based on photoswitchable molecules that can switch between a bright, fluorescent and a dark, nonfluorescent state. Some of these techniques, as well as their limitations, are discussed.


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