ii)-Carborane Complex Within a Tumour Cell Using Synchrotron XRF Imaging">
Australian Journal of Chemistry Australian Journal of Chemistry Society
An international journal for chemical science
RESEARCH FRONT

Uptake and Distribution of a Platinum(ii)-Carborane Complex Within a Tumour Cell Using Synchrotron XRF Imaging

Ellen L. Crossley A , Jade B. Aitken A , Stefan Vogt B , Hugh H. Harris C and Louis M. Rendina A D

A School of Chemistry, The University of Sydney, Sydney, NSW 2006, Australia.

B X-ray Science Division, Argonne National Laboratory, Argonne, IL 60439, USA.

C School of Chemistry and Physics, The University of Adelaide, SA 5005, Australia.

D Corresponding author. Email: lou.rendina@sydney.edu.au

Australian Journal of Chemistry 64(3) 253-257 http://dx.doi.org/10.1071/CH10453
Submitted: 10 December 2010  Accepted: 14 February 2011   Published: 11 March 2011

Abstract

Treatment of A549 human lung carcinoma cells with a DNA metallointercalator complex containing a PtII-terpy (terpy = 2,2′:6′,2′′-terpyridine) unit linked to a functionalized closo-carborane cage results in the uptake of the complex within the cells, as determined by synchrotron X-ray fluorescence (XRF) imaging. Although a significant cellular uptake of Pt existed, there was no significant accumulation of the element within the cell nuclei. Other statistically significant changes from the XRF data included an increase in Cl, K, and Cu and a decrease in Fe within the treated cells.

Graphical Abstract Image


References

[1]  R. F. Barth, J. A. Coderre, M. G. H. Vicente, T. E. Blue, Clin. Cancer Res. 2005, 11, 3987.
         | CrossRef | 1:CAS:528:DC%2BD2MXks1Gksr4%3D&md5=d8a81cdd7d8e38d5416ba0876aaa8adeCAS | 15930333PubMed | open url image1

[2]  E. L. Crossley, E. J. Ziolkowski, J. A. Coderre, L. M. Rendina, Mini Rev. Med. Chem. 2007, 7, 303.
         | CrossRef | 1:CAS:528:DC%2BD2sXitVOitLw%3D&md5=a4fbccd16ea77788e5ac485f2a81b63dCAS | 17346220PubMed | open url image1

[3]  A. V. Klein, T. W. Hambley, Chem. Rev. 2009, 109, 4911.
         | CrossRef | 1:CAS:528:DC%2BD1MXhtVGktbrK&md5=b6baacb9012bd5540685b0cee64b20b9CAS | 19711978PubMed | open url image1

[4]  J. Gao, Y.-G. Liu, R. A. Zingaro, Chem. Res. Toxicol. 2009, 22, 1705.
         | CrossRef | 1:CAS:528:DC%2BD1MXhtVWqtrjN&md5=d6223474b1569e0498ef783f5453cf20CAS | 19694485PubMed | open url image1

[5]  A. M. Krause-Heuer, R. Gruenert, S. Kuehne, M. Buczkowska, N. J. Wheate, D. D. Le Pevelen, L. R. Boag, D. M. Fisher, J. Kasparkova, J. Malina, P. J. Bednarski, V. Brabec, J. R. Aldrich-Wright, J. Med. Chem. 2009, 52, 5474.
         | CrossRef | 1:CAS:528:DC%2BD1MXpsFarsL8%3D&md5=e6d4a8bbd9305db053a8ac50dc54d719CAS | 19658404PubMed | open url image1

[6]  S. Ahmad, A. A. Isab, S. Ali, Transition Met. Chem. 2006, 31, 1003.
         | CrossRef | 1:CAS:528:DC%2BD28Xht1Wns7vK&md5=087638efc4785cc9128a52943636b2a1CAS | open url image1

[7]  M. Crul, R. C. A. M. van Waardenburg, J. H. Beijnen, J. H. M. Schellens, Cancer Treat. Rev. 2002, 28, 291.
         | CrossRef | 1:CAS:528:DC%2BD38XpsFyhu7Y%3D&md5=981c36dc3c1072e5673fbf35bf2c9d2fCAS | 12470980PubMed | open url image1

[8]  A. Ghezzi, M. Aceto, C. Cassino, E. Gabano, D. Osella, J. Inorg. Biochem. 2004, 98, 73.
         | CrossRef | 1:CAS:528:DC%2BD3sXpsVSqtro%3D&md5=4b7b50d10106ed58e8b151d3cbdde70cCAS | 14659635PubMed | open url image1

[9]  E. Wong, C. M. Giandomenico, Chem. Rev. 1999, 99, 2451.
         | CrossRef | 1:CAS:528:DyaK1MXltFSrsrc%3D&md5=506e9eb65532b283e8861687fd76fd2cCAS | 11749486PubMed | open url image1

[10]  M. D. Hall, C. T. Dillon, M. Zhang, P. Beale, Z. Cai, B. Lai, A. P. J. Stampfl, T. W. Hambley, J. Biol. Inorg. Chem. 2003, 8, 726.
         | CrossRef | 1:CAS:528:DC%2BD3sXnt1KisLc%3D&md5=33a8b77d6d7d6e287536e46df534a2c3CAS | 12884089PubMed | open url image1

[11]  C. T. Dillon, P. A. Lay, B. J. Kennedy, A. P. J. Stampfl, Z. Cai, P. Ilinski, W. Rodrigues, D. G. Legnini, B. Lai, J. Maser, J. Biol. Inorg. Chem. 2002, 7, 640.
         | CrossRef | 1:CAS:528:DC%2BD38XlsVClt7s%3D&md5=51c5b300dd6782b37ee20a572c9ba116CAS | 12072970PubMed | open url image1

[12]  H. H. Harris, A. Levina, C. T. Dillon, I. Mulyani, B. Lai, Z. Cai, P. A. Lay, J. Biol. Inorg. Chem. 2005, 10, 105.
         | CrossRef | 1:CAS:528:DC%2BD2MXjtVWmt7c%3D&md5=d5d2533b929d34500b07f77a38dbd422CAS | 15714299PubMed | open url image1

[13]  H. H. Harris, S. Vogt, H. Eastgate, P. A. Lay, J. Biol. Inorg. Chem. 2008, 13, 303.
         | CrossRef | 1:CAS:528:DC%2BD1cXhtV2ntbk%3D&md5=7761519f078e705531f3435172e9eca8CAS | 18034269PubMed | open url image1

[14]  M. Ralle, S. Lutsenko, Biometals 2009, 22, 197.
         | CrossRef | 1:CAS:528:DC%2BD1MXht1Gntb0%3D&md5=41b4706439ed6841b3ba4401c414d154CAS | 19130257PubMed | open url image1

[15]  B. S. Twining, S. B. Baines, N. S. Fisher, J. Maser, S. Vogt, C. Jacobsen, A. Tovar-Sanchez, S. A. Sanudo-Wilhelmy, Anal. Chem. 2003, 75, 3806.
         | CrossRef | 1:CAS:528:DC%2BD3sXkvFWmtbg%3D&md5=042f8bcb1b72836b647b197454d8034fCAS | 14572047PubMed | open url image1

[16]  T. Paunesku, S. Vogt, J. Maser, B. Lai, G. Woloschak, J. Cell. Biochem. 2006, 99, 1489.
         | CrossRef | 1:CAS:528:DC%2BD28Xht1Gks7nN&md5=bb7922fb886ff489801e982d1ca3d141CAS | 17006954PubMed | open url image1

[17]  C. J. Fahrni, Curr. Opin. Chem. Biol. 2007, 11, 121.
         | CrossRef | 1:CAS:528:DC%2BD2sXjvFegtb0%3D&md5=c47c73e13f553d4b7debd5948f73b176CAS | 17353139PubMed | open url image1

[18]  M. D. Hall, R. A. Alderden, M. Zhang, P. J. Beale, Z. Cai, B. Lai, A. P. J. Stampfl, T. W. Hambley, J. Struct. Biol. 2006, 155, 38.
         | CrossRef | 1:CAS:528:DC%2BD28XlvF2nsb8%3D&md5=53079a3ca8710a845462244424f0996fCAS | 16630726PubMed | open url image1

[19]  K. G. Chen, J. C. Valencia, B. Lai, G. Zhang, J. K. Paterson, F. Rouzaud, W. Berens, S. M. Wincovitch, S. H. Garfield, R. D. Leapman, V. J. Hearing, M. M. Gottesman, Proc. Natl. Acad. Sci. USA 2006, 103, 9903.
         | CrossRef | 1:CAS:528:DC%2BD28XmvVajtLg%3D&md5=b4184150ffe04a3b297794f86009c5f1CAS | open url image1

[20]  M. Shimura, A. Saito, S. Matsuyama, T. Sakuma, Y. Terui, K. Ueno, H. Yumoto, K. Yamauchi, K. Yamamura, H. Mimura, Y. Sano, M. Yabashi, K. Tamasaku, K. Nishio, Y. Nishino, K. Endo, K. Hatake, Y. Mori, Y. Ishizaka, T. Ishikawa, Cancer Res. 2005, 65, 4998.
         | CrossRef | 1:CAS:528:DC%2BD2MXltFemtLw%3D&md5=be84979e999d6a73fb96a65952079527CAS | 15958539PubMed | open url image1

[21]  J. B. Waern, H. H. Harris, B. Lai, Z. Cai, M. M. Harding, C. T. Dillon, J. Biol. Inorg. Chem. 2005, 10, 443.
         | CrossRef | 1:CAS:528:DC%2BD2MXhtVClt7%2FL&md5=daef9f2afb9f7e5980d3350df82ed316CAS | 15906108PubMed | open url image1

[22]  L. Yang, R. McRae, M. M. Henary, R. Patel, B. Lai, S. Vogt, C. J. Fahrni, Proc. Natl. Acad. Sci. USA 2005, 102, 11179.
         | CrossRef | 1:CAS:528:DC%2BD2MXoslKisb0%3D&md5=27b06b1254e69fe40366fd4ecc47247bCAS | open url image1

[23]  S. Corezzi, L. Urbanelli, P. Cloetens, C. Emiliani, L. Helfen, S. Bohic, F. Elisei, D. Fioretto, Anal. Biochem. 2009, 388, 33.
         | CrossRef | 1:CAS:528:DC%2BD1MXktFamtrY%3D&md5=23750ac6cdb031717bd8cba0de8fe8cfCAS | 19454226PubMed | open url image1

[24]  J. A. Todd, P. Turner, E. J. Ziolkowski, L. M. Rendina, Inorg. Chem. 2005, 44, 6401.
         | CrossRef | 1:CAS:528:DC%2BD2MXntVOksbo%3D&md5=41f4fdf282b5619f1f77764b5c0a98f5CAS | 16124820PubMed | open url image1

[25]  J. A. Todd, L. M. Rendina, Inorg. Chem. 2002, 41, 3331.
         | CrossRef | 1:CAS:528:DC%2BD38XktVaiu74%3D&md5=946eca22c5e6c77ca90aad0c8513d6bdCAS | 12079447PubMed | open url image1

[26]  E. L. Crossley, D. Caiazza, L. M. Rendina, Dalton Trans. 2005, 2825.
         | CrossRef | 1:CAS:528:DC%2BD2MXntlSisLY%3D&md5=d37efb8136eda070188647c18f52ac96CAS | 16094468PubMed | open url image1

[27]  F. J. Meyer-Almes, D. Porschke, Biochemistry 1993, 32, 4246.
         | CrossRef | 1:CAS:528:DyaK3sXitVCrs7c%3D&md5=7ce6ffd546007428e403e0899fae0246CAS | 8476852PubMed | open url image1

[28]  J. M. Benevides, G. J. Thomas, Biochemistry 2005, 44, 2993.
         | CrossRef | 1:CAS:528:DC%2BD2MXps1Kkuw%3D%3D&md5=772ef6e03d8f10b656b202e55d6799d6CAS | 15723542PubMed | open url image1

[29]  K. W. Jennette, S. J. Lippard, G. A. Vassiliades, W. R. Bauer, Proc. Natl. Acad. Sci. USA 1974, 71, 3839.
         | CrossRef | 1:CAS:528:DyaE2MXpsVeksQ%3D%3D&md5=6841972ad0717cf10747d1db97423dfeCAS | open url image1

[30]  E. L. Crossley, J. B. Aitken, S. Vogt, H. H. Harris, L. M. Rendina, Angew. Chem. Int. Ed. 2010, 49, 1231.
         | CrossRef | 1:CAS:528:DC%2BC3cXhslWluro%3D&md5=1a464e561891926e02db12240f41f5b7CAS | open url image1

[31]  C. D. Ferris, S. R. Jaffrey, A. Sawa, M. Takahashi, S. D. Brady, R. K. Barrow, S. A. Tysoe, H. Wolosker, D. E. Baranano, S. Dore, K. D. Poss, S. H. Snyder, Nat. Cell Biol. 1999, 1, 152.
         | CrossRef | 1:CAS:528:DyaK1MXks1Ogsb0%3D&md5=925bc67552eb43503ffab7bdd3439da2CAS | 10559901PubMed | open url image1

[32]  A. Calzolari, V. Finisguerra, I. Oliviero, S. Deaglio, G. Mariani, F. Malavasi, U. Testa, Blood Cells Mol. Dis. 2009, 42, 5.
         | CrossRef | 1:CAS:528:DC%2BD1cXhsFCgsL3P&md5=79df266b799c0cfb710a355840a3c6b6CAS | 19019709PubMed | open url image1

[33]  H. Chen, M. Costa, Exp. Biol. Med. 2006, 231, 1474.
         | 1:CAS:528:DC%2BD28XhtVylt77L&md5=ac1f73f0e0095ac7660a4f224583fcb1CAS | open url image1

[34]  D. E. Morrison, F. Issa, M. Bhadbhade, L. Gröebler, P. K. Witting, M. Kassiou, P. J. Rutledge, L. M. Rendina, J. Biol. Inorg. Chem. 2010, 15, 1305.
         | CrossRef | 1:CAS:528:DC%2BC3cXpvVWhu7c%3D&md5=43dbb11de452503a54e36c821e6189f7CAS | 20694490PubMed | open url image1

[35]  P. Ilinski, B. Lai, Z. Cai, W. Yun, D. Legnini, T. Talarico, M. Cholewa, L. K. Webster, G. B. Deacon, S. Rainone, D. R. Phillips, A. P. J. Stampfl, Cancer Res. 2003, 63, 1776.
         | 1:CAS:528:DC%2BD3sXivFynsrY%3D&md5=6456f32ddbd2afcf2def1979ccc06f08CAS | 12702562PubMed | open url image1

[36]  S. Vogt, J. Phys. IV 2003, 104, 635.
         | CrossRef | 1:CAS:528:DC%2BD3sXksVansLk%3D&md5=d834efc3dfea80d965332c84753784afCAS | open url image1

[37]  P. van Espen, Handbook of X-Ray Spectrometry: Revised and Expanded 2002 (Eds R. van Grieken, A. Markowicz) (Dekker: New York, NY).



Supplementary MaterialSupplementary Material (3.6 MB) Export Citation