Australian Journal of Chemistry Australian Journal of Chemistry Society
An international journal for chemical science
RESEARCH ARTICLE

Focus on Super-Resolution Imaging with Direct Stochastic Optical Reconstruction Microscopy (dSTORM)

Donna R. Whelan A , Thorge Holm B , Markus Sauer B and Toby D. M. Bell A C

A School of Chemistry, Monash University, Wellington Road, Clayton, Vic. 3800, Australia.

B Julius-Maximilians University of Würzburg, Am Hubland, 97074 Würzburg, Germany.

C Corresponding author. Email: toby.bell@monash.edu

Australian Journal of Chemistry 67(2) 179-183 http://dx.doi.org/10.1071/CH13499
Submitted: 19 September 2013  Accepted: 20 November 2013   Published: 13 December 2013

Abstract

The last decade has seen the development of several microscopic techniques capable of achieving spatial resolutions that are well below the diffraction limit of light. These techniques, collectively referred to as ‘super-resolution’ microscopy, are now finding wide use, particularly in cell biology, routinely generating fluorescence images with resolutions in the order of tens of nanometres. In this highlight, we focus on direct Stochastic Optical Reconstruction Microscopy or dSTORM, one of the localisation super-resolution fluorescence microscopy techniques that are founded on the detection of fluorescence emissions from single molecules. We detail how, with minimal assemblage, a highly functional and versatile dSTORM set-up can be built from ‘off-the-shelf’ components at quite a modest budget, especially when compared with the current cost of commercial systems. We also present some typical super-resolution images of microtubules and actin filaments within cells and discuss sample preparation and labelling methods.


References

[1]  E. Abbe, Arch. Mikrosk. Anat. 1873, 9, 413.
         | CrossRef | open url image1

[2]  L. Rayleigh, Philos. Mag. 1896, 42, 167. open url image1

[3]  B. N. G. Giepmans, S. R. Adams, M. H. Ellisman, R. Y. Tsien, Science 2006, 312, 217.
         | CrossRef | 1:CAS:528:DC%2BD28XjtlOjs70%3D&md5=39e6edd26f98cc4e9de72cbc4e412e51CAS | open url image1

[4]  M. Fernandez-Suarez, A. Y. Ting, Nat. Rev. Mol. Cell Biol. 2008, 9, 929.
         | CrossRef | 1:CAS:528:DC%2BD1cXhsVWhu7jK&md5=78cad8d5f229898250b111322c7be5efCAS | 19002208PubMed | open url image1

[5]  see editorial: Nat. Methods 2009, 6, 1.
         | CrossRef | open url image1

[6]  M. J. Rust, M. Bates, X. W. Zhuang, Nat. Methods 2006, 3, 793.
         | CrossRef | 1:CAS:528:DC%2BD28XpvVCmtLY%3D&md5=b15671659cdac25a263436c6ab2e2112CAS | 16896339PubMed | open url image1

[7]  S. T. Hess, T. P. K. Girirajan, M. D. Mason, Biophys. J. 2006, 91, 4258.
         | CrossRef | 1:CAS:528:DC%2BD28Xht1Khur%2FN&md5=e597a16059925329fe239529c409a5d6CAS | 16980368PubMed | open url image1

[8]  E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, H. F. Hess, Science 2006, 313, 1642.
         | CrossRef | 1:CAS:528:DC%2BD28XpsVOktL0%3D&md5=c5d5b3d44ff72d7655d9921b1a889d2eCAS | 16902090PubMed | open url image1

[9]  M. Sauer, J. Cell Sci. 2013, 126, 3505.
         | CrossRef | 1:CAS:528:DC%2BC3sXhsFelu7fN&md5=e54abd210ac061ab3583feac048d6500CAS | 23950110PubMed | open url image1

[10]  D. J. Williamson, D. M. Owen, J. Rossy, A. Magenau, M. Wehrmann, J. J. Gooding, K. Gaus, Nat. Immunol. 2011, 12, 655.
         | CrossRef | 1:CAS:528:DC%2BC3MXntV2ku7k%3D&md5=65c0b1534326d06f37a55717c1442c39CAS | 21642986PubMed | open url image1

[11]  J. Rossy, D. M. Owen, D. J. Williamson, Z. M. Yang, K. Gaus, Nat. Immunol. 2013, 14, 82.
         | CrossRef | 1:CAS:528:DC%2BC38XhslGkur%2FJ&md5=d9c7dd42b30558df473f5981e71d47b9CAS | 23202272PubMed | open url image1

[12]  M. Heilemann, S. van de Linde, M. Schuttpelz, R. Kasper, B. Seefeldt, A. Mukherjee, P. Tinnefeld, M. Sauer, Angew. Chem. Int. Ed. 2008, 47, 6172.
         | CrossRef | 1:CAS:528:DC%2BD1cXpvFSgtro%3D&md5=56d57387c48419e4963798a79e067246CAS | open url image1

[13]  S. van de Linde, S. Wolter, M. Sauer, Aust. J. Chem. 2011, 64, 503.
         | CrossRef | 1:CAS:528:DC%2BC3MXmvFyhs7s%3D&md5=4d70292fbe7c86ba3beb82dc9b0d3428CAS | open url image1

[14]  S. Wolter, U. Endesfelder, S. van de Linde, M. Heilemann, M. Sauer, Opt. Express 2011, 19, 7020.
         | CrossRef | 21503016PubMed | open url image1

[15]  S. Wolter, A. Loschberger, T. Holm, S. Aufmkolk, M. C. Dabauvalle, S. van de Linde, M. Sauer, Nat. Methods 2012, 9, 1040.
         | CrossRef | 1:CAS:528:DC%2BC38Xhs1ajt7rF&md5=14263117110d7acf1fc0e675b449bd2dCAS | 23132113PubMed | open url image1

[16]  S. van de Linde, A. Loschberger, T. Klein, M. Heidbreder, S. Wolter, M. Heilemann, M. Sauer, Nat. Protoc. 2011, 6, 991.
         | CrossRef | 1:CAS:528:DC%2BC3MXotlCnu7Y%3D&md5=8677d15602792cba92046cad729596abCAS | 21720313PubMed | open url image1

[17]  K. I. Mortensen, L. S. Churchman, J. A. Spudich, H. Flyvbjerg, Nat. Methods 2010, 7, 377.
         | CrossRef | 1:CAS:528:DC%2BC3cXkt1aqu7k%3D&md5=5c48cc8ab368a84ed44db76d8232e29bCAS | 20364147PubMed | open url image1

[18]  C. Flors, C. N. J. Ravarani, D. T. F. Dryden, ChemPhysChem 2009, 10, 2201.
         | CrossRef | 1:CAS:528:DC%2BD1MXhtV2itrzE&md5=53a9a4c0d6f6970657796b747f6bbd5aCAS | 19554598PubMed | open url image1

[19]  I. Schoen, J. Ries, E. Klotzsch, H. Ewers, V. Vogel, Nano Lett. 2011, 11, 4008.
         | CrossRef | 1:CAS:528:DC%2BC3MXhtVemu7jL&md5=7e25998680fbf712fb35d54ba0e2f5aaCAS | 21838238PubMed | open url image1

[20]  A. Sharonov, R. M. Hochstrasser, Proc. Natl. Acad. Sci. USA 2006, 103, 18911.
         | CrossRef | 1:CAS:528:DC%2BD28XhtlChu77N&md5=7447c8514c35ffab554d3a2b08d484f8CAS | 17142314PubMed | open url image1

[21]  T. Holm, T. Klein, A. Löschberger, T. Klamp, G. Wiebusch, S. van de Linde, M. Sauer, ChemPhysChem
         | CrossRef | open url image1

[22]  K. Xu, H. P. Babcock, X. W. Zhuang, Nat. Methods 2012, 9, 185.
         | CrossRef | 1:CAS:528:DC%2BC38XlsF2quw%3D%3D&md5=afbe4637db06d5c6d31f8d9556d7f1f0CAS | 22231642PubMed | open url image1


Full Text PDF (938 KB) Export Citation Cited By (1)