Backcross reciprocal monosomic analysis of leaf relative water content, stomatal resistance, and carbon isotope discrimination in wheat under pre-anthesis water-stress conditionsShahram Mohammady-D A C , Keith Moore B , John Ollerenshaw B and Behrooz Shiran A
A Faculty of Agriculture, University of Shahrekord, Iran.
B School of Biology, University of Newcastle upon Tyne, NE1 7RU, UK.
C Corresponding author. Email: firstname.lastname@example.org
Australian Journal of Agricultural Research 56(10) 1069-1077 https://doi.org/10.1071/AR05038
Submitted: 2 February 2005 Accepted: 3 August 2005 Published: 25 October 2005
Monosomic plants from an Australian variety (Oxley) having low stomatal resistance (SR), low leaf relative water content (LRWC), and high carbon isotope discrimination (Δ) were crossed with variety Falchetto having opposite characters in order to produce F2 backcross reciprocal monosomic families. The families were assessed under pre-anthesis water-stress conditions in a controlled growth chamber. F2 backcross reciprocal monosomic analysis suggested possible allelic variations between chromosomes 1A, 3A, 6A, 7A, 7B, 1D, and 4D of Falchetto and their homologues in Oxley for LRWC. This analysis also suggested possible allelic variation between chromosomes 5A, 1A, and 3A of Falchetto and their homologues in Oxley for SR. Extending the analysis to the F3 disomic generation and the assessment of LRWC at this generation confirmed that reciprocals for chromosomes 3A and 6A showed significant differences. F2 backcross reciprocal monosomic analysis for Δ suggested allelic variations on chromosomes 1D, 4D, and 5D. However, chromosome 1D from Falchetto had the highest difference from its homologue in Oxley. Assessing the reciprocals of this chromosome for vegetative evapotranspiration efficiency (ETEveg) at the F3 disomic generation indicated that the observed variation for Δ was translated into differences for ETEveg. These results indicate that chromosome 1D of Falchetto is promising in reducing Δ and that the improvement of wheat varieties for ETEveg can be done by selection for Δ. Finally, plieotropic effects of some chromosomes were observed for the characters under study. This suggests the existence of genetic factors on these chromosomes affecting more than one character. However, some pleiotropic effects could also be due to non-genetic developmental interactions.
Additional keywords: disomic, plieotropic effect, within-family variation.
We thank Prof. J. Snape and the late T. Worland, John Innes Centre, Norwich, UK, for their valuable comments on the experiments, and Prof. R. A. McIntosh for providing the monosomic lines.
Adjei GB, Kirkham MB (1980) Evaluation of winter wheat cultivars for drought resistance. Euphytica 29, 155–160.
| CrossRef |
Araus JL, Slafer GA, Reynolds MP, Royo C (2002) Plant breeding and drought in C-3 cereals: What should we breed for? Annals of Botany 89, 925–940.
| CrossRef | PubMed |
Blum A, Gozlan G, Mater J (1981) Manifestation of dehydration avoidance in wheat breeding germplasm. Crop Science 21, 495–499.
Blum A, Johnson JW (1993) Wheat cultivars respond differently to a drying top-soil and a possible nonhydraulic root signal. Journal of Experimental Botany 44, 1149–1153.
Boutton TW (1991) Stable carbon isotope ratios of natural materials. I. Sample preparation and mass spectrometric analysis. ‘Carbon isotope techniques’. (Eds DG Coleman, B Fry) pp. 235–255. (Academic Press: London)
Buerstmayr H, Lemmens M, Feda GK, Ruckenbauer P (1999) Back-cross reciprocal monosomic analysis of Fusarium head blight resistance in wheat (Triticum aestivum L.). Theoretical and Applied Genetics 98, 76–85.
| CrossRef |
Chaves MM (1991) Effects of water deficits on carbon assimilation. Journal of Experimental Botany 42, 1–16.
Chojecki AJS, Gale MD, Bayliss MW (1983) Reciprocal monosomic analysis of grain size in wheat. ‘Proceedings of 6th International Wheat Genetic Symposium’. (Ed. S Sakamoto ) pp. 1061–1071. (Kyoto University: Kyoto, Japan)
Dhanda SS, Sethi GS (1998) Inheritance of excised-leaf water loss and relative water content in bread wheat (Triticum aestivum). Euphytica 104, 39–47.
| CrossRef |
Ehdaie B (1995) Variation in water use efficiency and its components in wheat II. Pot and field experiments. Crop Science 35, 1617–1629.
Ehdaie B, Waines JG (1994) Genetic-analysis of carbon isotope discrimination and agronomic characters in a bread wheat cross. Theoretical and Applied Genetics 88, 1023–1028.
| CrossRef |
Ehdaie B, Waines JG (1997) Chromosomal location of genes influencing plant characters and evapotranspiration efficiency ratio in bread wheat. Euphytica 96, 363–375.
| CrossRef |
Farquhar GD, Richards RA (1984) Isotopic composition of plant carbon correlates with water use efficiency of wheat genotypes. Australian Journal of Plant Physiology 11, 539–552.
Farshadfar E, Koszegi B, Tischner T, Sutka J (1995) Substitution analysis of drought tolerance in wheat (Tritium aestivum). Plant Breeding 114, 542–544.
Giura A, Saulescu NN (1996) Chromosomal location of genes controlling grain size in a large grained selection of wheat (Triticum aestivum L). Euphytica 89, 77–80.
| CrossRef |
Golestani Araghi S, Assad MT (1998) Evaluation of four screening techniques for drought resistance and their relationship to yield reduction ratio in wheat. Euphytica 103, 293–299.
| CrossRef |
Griffiths H (1993) Carbon isotope discrimination. ‘Photosynthesis and production in a changing environment: a field and laboratory manual’. (Eds DO Hall, JMO Scurlock, HR Bolhar-Nordenkampf, RC Leegood, S Long) pp. 181–192. (Chapman and Hall: London)
Gumuluru S, Hobbs SLA, Jana S (1989) Genotypic variability in physiological characters and its relationship to drought tolerance in durum wheat. Canadian Journal of Plant Science 69, 703–711.
Jones H (1987) Breeding for stomatal characters. ‘Stomatal function’. (Eds E Zeiger, GD Farquhar, IR Cowan) pp. 431–443. (Stanford University Press: Stanford, CA)
Law CN, Snape JW, Worland AJ (1987) Aneuploidy in wheat and its uses in genetic analysis. ‘Wheat breeding: its scientific basis’. (Ed. FGH Lupton) pp. 71–108. (Chapman and Hall: New York)
Law CN, Worland AJ (1996) Inter-varietal chromosome substitution lines in wheat—revisited. Euphytica 89, 1–10.
| CrossRef |
Leven H (1960) Robust tests for equality of variances. ‘Contributions to probability and statistics: essay in honour of Harold Hotelling’. (Eds I Olkin, SG Ghurye, W Hoeffding, WG Madow, HB Mann) (Stanford University Press: London)
Mentewab A, Sarrafi A (1998) Performance of androgenic doubled haploid spring wheat lines for excise-leaf water status and agronomic traits in comparison with their parents. Cereal Research Communications 26, 137–143.
Mohammady-D S (2002) Inheritance of tolerance to water-stress in wheat (Triticum aestivum). PhD thesis, University of Newcastle upon Tyne, UK.
Mohammady-D S (2004) Chromosome 1D as a possible location of a gene controlling carbon isotope discrimination (Δ) in wheat (Triticum aestivum) under water-stress conditions. ‘Genetic variation for plant breeding’. (Ed. J Vollman) pp. 367–370. (BOKU: Vienna)
Rebetzke GJ, Condon AG, Richards RA, Farquhar GD (2002) Selection for reduced carbon isotope discrimination increases aerial biomass and grain yield of rain fed bread wheat. Crop Science 42, 739–745.
Richards RA, Rebetzke GJ, Condon AG (1998) Genetic improvement of water-use efficiency and yield of dryland wheat. ‘Proceedings of 9th International Wheat Genetic Symposium’. (Ed. AE Slinkard ) pp. 57–60. (Saskatchewan University: Canada)
Schonfeld MA, Johnson RC, Carver BF (1988) Water relation in winter wheat as drought resistance indicators. Crop Science 28, 526–531.
Shimshi D, Ephrat J (1975) Stomatal behaviour of wheat cultivars in relation to their transpiration, photosynthesis and yield. Agronomy Journal 67, 326–331.
Snape JW, Law CN (1980) The detection of homologous chromosome variation in wheat using backcross reciprocal monosomic lines. Heredity 45, 187–200.
Snape JW, Parker BB, Gale MD (1983) Use of the backcross reciprocal monosomic method for evaluation chromosomal variation for quantitative characters. ‘Proceedings of 6th International Wheat Genetics Symposium’. (Ed. S Sakamoto ) pp. 367–373. (Kyoto University: Kyoto, Japan)
Steel, RG , and Torrie, D (1976).
Taiz, I , and Zeiger, E (1998).
Wang H, Clarke JM (1993) Relationship of excised-leaf water-loss and stomatal frequency in wheat. Canadian Journal of Plant Science 73, 93–99.
Winter SR, Musick JT, Porter KB (1988) Evaluation of screening techniques for breeding drought tolerant winter wheat. Crop Science 28, 512–516.
Zadoks JC, Change TT, Knozak CF (1974) A decimal code for growth stages of cereals. Weed Research 14, 415–421.