Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality
REVIEW

The epidemiology and control of ascochyta blight in field peas: a review

T. W. Bretag A D , P. J. Keane B and T. V. Price C

A Primary Industries Research Victoria, Department of Primary Industries, Private Bag 260, Horsham, Vic. 3401, Australia.

B Department of Botany, La Trobe University, Vic. 3086, Australia.

C Department of Agriculture, La Trobe University, Vic. 3086, Australia.

D Corresponding author. Email: trevor.bretag@dpi.vic.gov.au

Australian Journal of Agricultural Research 57(8) 883-902 http://dx.doi.org/10.1071/AR05222
Submitted: 23 June 2005  Accepted: 2 February 2006   Published: 9 August 2006

Abstract

Ascochyta blight is one of the most important diseases affecting field peas. The disease occurs in almost all pea-growing regions of the world and can cause significant crop losses when conditions are favourable for an epidemic. Here we review current knowledge of the epidemiology of the disease. Details are provided of disease symptoms, the disease cycle and the taxonomy of the causal fungi, Ascochyta pisi, Mycosphaerella pinodes and Phoma pinodella.

The importance of seed-, soil- and air-borne inoculum is discussed along with the factors that influence survival of the causal fungi in soil, on seed or associated with pea trash. Many studies have been reviewed to establish how the fungi responsible for the disease survives from year to year, how the disease becomes established in new crops and the conditions that favour disease development. Evidence is provided that crop rotation, destruction of infected pea trash and chemical seed treatments can significantly reduce the amount of primary inoculum. Later sowing of crops has been shown to reduce the incidence and severity of disease. Fungicides have been used successfully to control the disease, although the cost of their application can significantly reduce the profitability of the crop. The best long-term strategy for effective disease control appears to be the development of ascochyta blight resistant pea varieties. Reports of physiological specialisation in ascochyta blight fungi are also documented. Despite extensive screening of germplasm, relatively few sources of resistance to ascochyta blight fungi have been found in Pisum sativum. However, the discovery of much better sources of resistance in closely related species and the development of advanced breeding methods offer new possibilities for developing useful resistance.

Additional keywords: chemical control, crop loss assessment, disease cycle, disease resistance, host-pathogen interactions, taxonomy.


References

Abd El Rehim MA Abo Taleb EM Michail SH Metwally SM 1997 Seed health testing of peas for Ascochyta spp. and their impact on foliage seed health and seed yield. Acta Phytopathologica et Entomologica Hungarica 32 89 95

Ali SM Nitschke LF Dube AJ Krause MR Cameron B 1978 Selection of pea lines for resistance to pathotypes of Ascochyta pinodes, A. pisi and Phoma medicaginis var. pinodella. Australian Journal of Agricultural Research 29 841 849
doi:10.1071/AR9780841

Ali SM Paterson J Crosby J 1982 A standard technique for detecting seed-borne pathogens in peas, chemical control, and testing commercial seed in South Australia. Australian Journal of Experimental Agriculture and Animal Husbandry 22 348 352 doi:10.1071/EA9820348

Ali SM Sharma B Ambrose MJ 1994 Current status and future strategy in breeding pea to improve resistance to biotic and abiotic stresses. Euphytica 73 115 126 doi:10.1007/BF00027188

Ali-Khan ST Zimmer RC Kenaschuk EO 1973 Reactions of pea introductions to Ascochyta foot rot and powdery mildew. Canadian Plant Disease Survey 53 155 156

Amit W Singh PP Sandhu KS Wadhwa A 2002 a Extraction of pisatin from pea leaves infected with Ascochyta pisi. Plant Disease Research 17 369 370


Amit W Singh PP Sandhu KS Wadhwa A 2002 b Factors affecting growth and sporulation of Ascochyta pisi. Plant Disease Research 17 371 374


Anderson AJ Powelson ML 1979 Production of plant cell wall degrading enzymes by Phoma medicaginis f. sp. pinodella. Phytopathology 69 372 375


Anderson JAD White JGH 1974 Yield of green peas. II. Effects of water and plant density. New Zealand Journal of Experimental Agriculture 2 165 171


Annapurna Y Rao PR 1979 A microbial study of Cicer arietinum L. leaves sprayed with some pesticides. Geobios 6 230 232


Anselme C , Hewett PD , Champion R (1970) The detection and importance of Ascochyta pisi Lib. on seed peas. In ‘Seventh International Congress of Plant Protection’. Paris, France. pp. 1–4.

Anwar SA Bhutta AR Rauf CA Khan MSA 1994 Seedborne fungi of pea and their role in poor germination of pea seed. Pakistan Journal of Phytopathology 6 135 139


Banniza S Vandenberg A 2003 The influence of plant injury on development of Mycosphaerella pinodes in field pea. Canadian Journal of Plant Pathology 25 304 311


Bathgate JA Sivasithamparam K Khan TN 1989 Identity and recovery of seed-borne fungal pathogens of field peas in Western Australia. New Zealand Journal of Crop and Horticultural Science 17 97 101


Baughan PJ , Toms AM (1984) The development of a complete seed coating package for peas. Proceedings British Plant Protection Conference - Pests and Diseases 10A–1, 965–970.

Baumann G 1955 Studies on the biology of Mycosphaerella pinodes (Berk. & Blox.) Stone. Zeitschrift fur Pflanzenkrankheiten 62 254 255


Beasse C Ney B Tivoli B 1999 Effects of pod infection by Mycosphaerella pinodes on yield components of pea (Pisum sativum). Annals of Applied Biology 135 359 367


Bhardwaj CL Thakur DR 2000 Dry grain yield in rainfed garden pea (Pisum sativum) in relation to environment and management of Mycosphaerella blight. Indian Journal of Agricultural Sciences 70 231 233


Biddle A 1989 Fungicides for peas. Vegetable Grower 1 12 13


Blakeman JP Dickinson CH 1967 The effect of ultraviolet and visible light on infection of host leaf tissue by four species of Ascochyta. Transactions of the British Mycological Society 50 385 396


Blixt S (1970) ‘Pisum.’ (Blackwell Scientific Publications: Oxford)

Blomfield PD 1954 Note on the molybdenum response on peas in Nelson. New Zealand Journal of Science 36 46


Boerema GH , Gruyter Jd , Noordeloos ME , Hamers MEC (2004) ‘Phoma identification manual. Differentiation of specific and infra-specific taxa in culture.’ (CABI Publishing: Wallingford, UK)

Bowen JK Peart J Lewis BG Cooper C Matthews P 1996 Development of monoclonal antibodies against the fungi of the ‘Ascochyta complex’. Plant Pathology 45 393 406
doi:10.1046/j.1365-3059.1996.d01-136.x

Boy N 1981 Local protection of pea against Mycosphaerella pinodes (Berk. & Blox.) by pre-inoculation with Ascochyta fabae (Speg.). Parasitica 37 109 118

Bretag TW 1985 Chemical control of ascochyta blight of field peas. Australasian Plant Pathology 14 42 43
doi:10.1071/APP9850042

Bretag TW (1991) Epidemiology and control of ascochyta blight of field peas. PhD thesis, La Trobe University, Australia.

Bretag TW (2004) ‘Review: Ascochyta blight in field peas.’ (Victorian Department of Primary Industries: Horsham, Vic.)

Bretag TW , Brouwer JB (1995) Effects of different plant phenotypes on the severity of ascochyta blight in field peas (Pisum sativum L.) in southern Australia. In ‘2nd European Conference on Grain Legumes: Improving production and utilization of grain legumes’. Copenhagen, Denmark. p. 92. (AEP)

Bretag TW Keane PJ Price TV 1995 a Effect of ascochyta blight on the grain yield of field peas (Pisum sativum L.) grown in southern Australia. Australian Journal of Experimental Agriculture 35 531 536 doi:10.1071/EA9950531

Bretag TW Price TV Keane PJ 1995 b Importance of seed-borne inoculum in the etiology of the ascochyta blight complex of field peas (Pisum sativum L.) grown in Victoria. Australian Journal of Experimental Agriculture 35 525 530 doi:10.1071/EA9950525

Bretag TW Keane PJ Price TV 2000 Effect of sowing date on the severity of ascochyta blight in field peas (Pisum sativum L.) grown in the Wimmera region of Victoria. Australian Journal of Experimental Agriculture 40 1113 1119 doi:10.1071/EA00097

Bretag TW , Ramsey MD (2001) Foliar diseases caused by fungi: Ascochyta spp. In ‘Compendium of pea diseases and pests’. (Eds JM Kraft, FL Pfleger) pp. 24–28. (APS Press: The American Phytopathological Society: St. Paul, MN)

Bretag TW , Smith LM , Ward DJ (2001) Effect of soil-borne ascochyta blight fungi on the grain yield of field peas. In ‘10th Australian Agronomy Conference’. Hobart, Tasmania. (Australian Society of Agronomy)

Brett CC Dillon Weston WAR Booer JR 1937 Seed disinfection. III. Experiments on the germination of peas. Seed protection by the use of disinfectant dusts containing mercury. Journal of Agriculture Science, Cambridge 27 53 66

Brewer D 1960 Studies in Ascochyta pisi Lib. Canadian Journal of Botany 38 706 717


Brewer D MacNeill BH 1953 Preliminary studies in Ascochyta pisi Lib. Canadian Journal of Botany 31 739 744


Brittain MJ (1987) The resistance of Pisum to Ascochyta pisi. PhD thesis, University of East Anglia, UK.

Brown JF (1980) ‘A course manual in plant protection.’ (Hedges & Bell Pty. Ltd.: Melbourne)

Carter MV 1959 Chemical destruction of mature perithecia of Mycosphaerella pinodes. Nature 183 162 163


Carter MV 1961 Report of Rothamsted Experimental Station for 1960. Ascospore release by Mycosphaerella pinodes from pea trash. Rothamsted Experimental Station Annual Report 1960 1 329


Carter MV 1963 Mycosphaerella pinodes: The phenology of ascospore release. Australian Journal of Biological Sciences 16 800 817


Carter MV Moller WJ 1960 Black spot of peas. Journal of Agriculture, South Australia 63 353 363


Carter MV Moller WJ 1961 Factors affecting the survival and dissemination of Mycosphaerella pinodes (Berk. & Blox.) Vestergr. in South Australian irrigated pea fields. Australian Journal of Agricultural Research 12 878 888
doi:10.1071/AR9610878

Cass Smith WP 1954 Plant diseases: Ascochyta blight of peas. Journal of Agriculture, Western Australia 3 711 713

Chen MH Huang JW Yein CF 1994 The pathway and influence factors in pod and seed infections of garden peas by Mycosphaerella pinodes. Plant Pathology Bulletin 3 133 139


Chung HS Wilcoxson RD 1969 Effect of conidial number and matrix on germination of conidia in Phoma medicaginis. Phytopathology 59 440 442


Chung HS Wilcoxson RD 1971 Effects of temperature, light, carbon and nitrogen nutrition on reproduction in Phoma medicaginis. Mycopathologia et Mycologia Applicata 44 297 308
doi:10.1007/BF02052703

Clulow SA (1989) The resistance of Pisum to Mycosphaerella pinodes (Berk. & Blox.) Vestergr. PhD thesis, University of East Anglia, UK.

Clulow SA Lewis BG Matthews P 1992 Expression of resistance to Mycosphaerella pinodes in Pisum sativum. Plant Pathology 41 362 369

Clulow SA Lewis BG Parker ML Matthews P 1991 a Infection of pea epicotyls by Mycosphaerella pinodes. Mycological Research 95 817 820


Clulow SA Matthews P Lewis BG 1991 b Genetical analysis of resistance to Mycosphaerella pinodes in pea seedlings. Euphytica 58 183 189
doi:10.1007/BF00022819

Cruickshank IAM (1954) Certification of pea seed. Commonwealth Mycological Conference Report 5, 1–159.

Cruickshank IAM 1957 A note on the incidence of Ascochyta species in pea seed. New Zealand Journal of Science and Technology 38 497 499

Cruickshank IAM 1963 Phytoalexins. Annual Review of Phytopathology 1 351 374
doi:10.1146/annurev.py.01.090163.002031

Csizmadia L 1995 Breeding peas for disease resistance: 2. Ascochyta leaf and pod spot. Horticultural Science 27 57 61

Dann EK Deverall BJ 2000 Activation of systemic disease resistance in pea by an avirulent bacterium or a benzothiadiazole, but not by a fungal leaf spot pathogen. Plant Pathology 49 324 332
doi:10.1046/j.1365-3059.2000.00457.x

Davidse LC 1986 Benzimidazole fungicides: Mechanism of action and biological impact. Annual Review of Phytopathology 24 43 65 doi:10.1146/annurev.py.24.090186.000355

Davidson JA Ramsey MD 2000 Pea yield decline syndrome in South Australia: the role of diseases and the impact of agronomic practices. Australian Journal of Agricultural Research 51 347 354 doi:10.1071/AR99111

Davies DR (1976) Peas: Pisum sativum (Leguminosae-Papilionatae). In ‘Evolution of crop plants’. (Ed. NW Simmonds) pp. 172–174. (Longman: London)

De Tempe J 1968 a An analysis of the laboratory testing requirements of two seed-borne diseases. Proceedings of the International Seed Testing Association 33 583 588

De Tempe J 1968 b The quantitative evaluation of seed-borne pathogenic infection. Proceedings of the International Seed Testing Association 33 573 581


Dickinson CH 1967 Fungal colonization of Pisum leaves. Canadian Journal of Botany 45 915 927


Dickinson CH Sheridan JJ 1968 Studies on the survival of Mycosphaerella pinodes and Ascochyta pisi. Annals of Applied Biology 62 473 483


Dirlewanger E Isaac PG Ranade S Belajouza M Cousin R de Vienne D 1994 Restriction fragment length polymorphism analysis of loci associated with disease resistance genes and developmental traits in Pisum sativum L. Theoretical and Applied Genetics 88 17 27
doi:10.1007/BF00222388

Dolgikh ST Krotova TI 1971 Induced mutants of pea with increased resistance to Ascochyta disease. Vestnik Sel Skokhozyaistevennoi Nauki Moskva 10 56 59

Duparque M , Baranger A , Tivoli B , Onfroy C , Boitel C , Morin G (1998) Investigating a new simple screening method for Ascochyta blight (Mycosphaerella pinodes) resistance applicable in field pea (Pisum sativum L.) breeding nurseries. In ‘3rd European Conference on Grain Legumes’. Valladolid, Spain. pp. 229–230. (AEP Publishing)

Durieu P Ochatt SJ 2000 Efficient intergeneric fusion of pea (Pisum sativum L.) and grass pea (Lathyrus sativus L.) protoplasts. Journal of Experimental Botany 51 1237 1242
doi:10.1093/jexbot/51.348.1237

Evidente A Capasso R Vurro M Bottalico A 1993 a Ascosalitoxin, a phytotoxic trisubstituted salicylic aldehyde from Ascochyta pisi. Phytochemistry 34 995 998 doi:10.1016/S0031-9422(00)90701-5

Evidente A Lanzetta R Capasso R Vurro M Bottalico A 1993 b Pinolidoxin, a phytotoxic nonenolide from Ascochyta pinodes. Phytochemistry 34 999 1003 doi:10.1016/S0031-9422(00)90702-7

Faris Mokaiesh S Boccara M Denis JB Derrien A Spire D 1996 Differentiation of the “Ascochyta complex” fungi of pea by biochemical and molecular markers. Current Genetics 29 182 190

Faris Mokaiesh S , Corbiere E , Bertrandy J , Spire D (1992) Studies on the variability of three Ascochyta species in pea. In ‘1st European Conference on Grain Legumes’. Angers, France. pp. 357–358. (AEP Publishing)

Faris Mokaiesh S Corbiere R Lyons NF Spire D 1995 Evaluation of an enzyme-linked immunosorbent assay for detection of Mycosphaerella pinodes in pea seeds. Annals of Applied Biology 127 441 455


Gadd I 1950 Hot-water treatment, a new method of controlling Ascochyta spp. on pea seeds. Proceedings of the International Seed Testing Association 16 1 94


Garry G Jeuffroy MH Ney B Tivoli B 1998 a Effects of Ascochyta blight (Mycosphaerella pinodes) on the photosynthesizing leaf area and the photosynthetic efficiency of the green leaf area of dried-pea (Pisum sativum). Plant Pathology 47 473 479


Garry G Jeuffroy MH Tivoli B 1998 b Effects of ascochyta blight (Mycosphaerella pinodes Berk. & Blox.) on biomass production, seed number and seed weight of dried-pea (Pisum sativum L.) as affected by plant growth stage and disease intensity. Annals of Applied Biology 132 49 59


Garry G Tivoli B Jeuffroy MH Citharel J 1996 Effects of ascochyta blight caused by Mycosphaerella pinodes on the translocation of carbohydrates and nitrogenous compounds from the leaf and hull to seed of dried pea. Plant Pathology 45 769 777
doi:10.1046/j.1365-3059.1996.d01-170.x

Gaumann E (1950) ‘Principles of plant infection.’ (Crosby Lockwood & Son, LTD.: London)

Gent GP (1988) ‘The PGRO pea growing handbook.’ (Processors and Growers Research Organisation: Thornhaugh)

Gretenkort MA Helsper J 1993 Disease assessment of pea lines with resistance to foot rot pathogens: protocols for in vitro selection. Plant Pathology 42 676 685

Grewal RK Jhooty JS Aulakh KS Thind TS Kaur J 1981 Effect of fungicides on seed-borne fungi in pea var. Bonneville under different storage conditions. Pesticides 15 23 24


Gritton ET (1980) Field pea. In ‘Hybridization of crop plants’. (Eds WR Fehr, HH Hadley) pp. 347–356. (American Society of Agronomy and Crop Science Society of America: Madison, WI)

Gurung AM Pang ECK Taylor PWJ 2002 Examination of Pisum and Lathyrus species as sources of ascochyta blight resistance for field pea (Pisum sativum). Australasian Plant Pathology 31 41 45
doi:10.1071/AP01069

Hare WW Walker JC 1944 Ascochyta diseases of canning pea. Wisconsin Agricultural Experimental Station, Research Bulletin 150 1 31

Harlan JR 1977 Sources of genetic defence. Annals of the New York Academy of Sciences 287 345 356


Hassan HH Cox CE 1947 Some effects of spergon as a pea seed treatment on soil fungi. Phytopathology 37 439


Heath MC Wood RKS 1969 Leaf spots induced by Ascochyta pisi and Mycosphaerella pinodes. Annals of Botany 33 657 670


Heath MC Wood RKS 1971 Role of cell-wall-degrading enzymes in the development of leaf spots caused by Ascochyta pisi and Mycosphaerella pinodes. Annals of Botany 35 451 474


Heenan DP 1994 Effects of sowing time on growth and grain yield of lupin and field pea in south-eastern New South Wales. Australian Journal of Experimental Agriculture 34 1137 1142
doi:10.1071/EA9941137

Hewett PD 1979 Pretreatment in seed health testing 2: Duration of hypochlorite pretreatment in the agar plate test for Ascochyta spp. Seed Science and Technology 7 83 85

Hillstrand DS Auld DL 1982 Comparative Evaluation of four techniques for screening winter peas for resistance to Phoma medicaginis var. pinodella. Crop Science 22 282 287


Hiramatsu M Ichinose Y Shiraishi T Oku H Ouchi S 1986 Regulation of pisatin biosynthesis in pea leaves by elicitor and suppressor produced by Mycosphaerella pinodes. Annals of the Phytopathological Society of Japan 52 53 58


Hoffman RM Turner JG 1982 Partial purification of proteins from pea leaflets that inhibit Ascochyta pisi endopolygalacturonase. Physiological Plant Pathology 20 173 187


Hoffman RM Turner JG 1984 Occurrence and specificity of an endopolygalacturonase inhibitor in Pisum sativum. Physiological Plant Pathology 24 49 59


Hosnedl V Ondrejova H 1982 Effect of seed dressing on production processes and health status of peas. Agrochemia 22 338 340


Jones FR Vaughan RE 1921 Anthracnose of the garden pea. Phytopathology 11 500 503


Jones LK 1927 Studies on the nature and control of blight, leaf and pod spot, and foot-rot of peas caused by species of Ascochyta. New York State Agricultural Experimental Station, Research Report 547 1 46


Kaur M Deshpande KB 1980 In vitro production of toxic metabolites by Ascochyta pisi (Pass.) Lab. inciting blight of peas (Pisum sativum L.). Acta Botanica Indica 8 210 214


Kerling LCP 1928 The anatomical structure of leaf spots. Mededelingen van de Landbouwhooge school te Wageningen 32 6 107


Kerling LCP 1949 Infection of peas by Mycosphaerella pinodes (Berk. & Blox.) Stone. Tijdschrift over Plantenziekten 55 41 68
doi:10.1007/BF01991842

Kiba A Toyoda K Ichinose Y Yamada T Shiraishi T 1996 Species-specific suppression of superoxide-anion generation on surfaces of pea leaves by the suppressor from Mycosphaerella pinodes. Annals of the Phytopathological Society of Japan 62 508 512

Kraft JM Dunne B Goulden D Armstrong S 1998 A search for resistance in peas to Mycosphaerella pinodes. Plant Disease 82 251 253


Lawyer AS (1984) Foliar diseases caused by fungi: diseases caused by Ascochyta spp. In ‘Compendium of pea diseases’. (Ed. DJ Hagedorn) pp. 11–15. (APS Press, The American Phytopathological Society: St. Paul, MN)

Le May C Schoeny A Tivoli B Ney B 2005 Improvement and validation of a pea crop growth model to simulate the growth of cultivars infected with Ascochyta blight. European Journal of Plant Pathology 112 1 12
doi:10.1007/s10658-004-5272-4

Le May C , Tivoli B , Ney B (2001) Effect of pea plant architecture on epidemic development of ascochyta blight and outcomes on yield. In ‘4th European Conference on Grain Legumes’. Cracow, Poland. (Ed. AEP) pp. 72–73. (AEP Publishing)

Leach CM 1959 Effect of visible and ultraviolet radiations on the sporulation of Ascochyta pisi and other seed-borne fungi. Phytopathology 49 543

Leach LD Snyder WC 1947 Localized chemical applications to the soil and their effects upon root rots of beans and peas. Phytopathology 37 363


Lepoivre P 1979 Protection of Pisum sativum against infection by Ascochyta pinodes by pre-inoculation with Ascochyta pisi, Ascochyta fabae or Ascochyta pinodes. Parasitica 35 73 78


Lepoivre P 1982 a Extraction of ascochitine from pea leaves [Pisum sativum] infected by Ascochyta pisi (Lib.) or Mycosphaerella pinodes (Berk. & Blox.) Vestergr. Parasitica 38 45 53


Lepoivre P 1982 b Sensitivity of pea cultivars to ascochitine and the possible role of the toxin in the pathogenicity of Ascochyta pisi (Lib.). Phytopathologische Zeitschrift 103 25 34


Lepoivre P Boy N 1983 Effect of pea lectin on the infection of pea by Mycosphaerella pinodes (Berk. & Blox.) Verstergr. Parasitica 39 145 149


Linford MB Sprague R 1927 Species of Ascochyta parasitic on the pea. Phytopathology 17 381 397


Louis I Cooke RC 1985 a Conidial matrix and spore germination on some plant pathogens. Transactions of the British Mycological Society 84 661 667


Louis I Cooke RC 1985 b Enzymes in conidial matrix of Colletotrichum gloeosporioides and Mycosphaerella pinodes. Transactions of the British Mycological Society 84 742 745


Madhosingh C Wallen VR 1968 Serological differentiation of the Ascochyta species of peas. Canadian Journal of Microbiology 14 449 451


Matthews D 1964 A note on the testing of pea seed for the presence of Ascochyta spp. Proceedings of the International Seed Testing Association 29 141 144


Matthews P (1989) Inheritance of disease resistance in Pisum. In ‘Report on the 2nd Meeting of the E.C. Pea Group’. Brussels. (Eds JF Sutcliffe, JS Pate) pp. 70–73. (Academic Press: London)

Maude RB 1966 Pea seed infection by Mycosphaerella pinodes and Ascochyta pisi and its control by seed soaks in thiram and captan suspensions. Annals of Applied Biology 57 193 200


Maude RB Kyle AM 1970 Seed treatments with benomyl and other fungicides for the control of Ascochyta pisi on peas. Annals of Applied Biology 66 37 41


Maude RB (1973) Seed-borne diseases and their control. In ‘Seed ecology’. (Ed. W Heydecker) pp. 325–335. (Butterworth & Co. Ltd.: London)

Maude RB Bambridge JM Spencer A 1986 a Tests of fungicide seed treatments to eliminate seed-borne Ascochyta pisi (leaf and pod spot of peas). Annals of Applied Biology 108 70 71
supplement Tests of Agrochemicals and Cultivars No. 7)

Maude RB Presly AH Lovett JF 1986 b Demonstration of the adherence of thiram to pea seeds using a rapid method of spectrophotometric analysis. Seed Science and Technology 14 361 369


McDonald GK Dean G 1996 Effect of waterlogging on the severity of disease caused by Mycosphaerella pinodes in peas (Pisum sativum L.). Australian Journal of Experimental Agriculture 36 219 222
doi:10.1071/EA9960219

Moore WC 1946 Seed-borne diseases. Annals of Applied Biology 33 228 231

Morrall RAA McKenzie DL Slinkard AE 1976 Evidence of disease tolerance to Ascochyta blight in a field pea cultivar. Proceedings of the Canadian Phytopathological Society 44 42


Nasir M Hoppe HH 1991 Studies on pathotype differentiation within Mycosphaerella pinodes (Berk. & Bloxam) Vestergren, a component of the Ascochyta-disease-complex of peas (Pisum sativum L.). Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz 98 619 626


Nasir M Hoppe HH 1998 Evaluation of seed treatments to control Mycosphaerella pinodes on peas. Annals of Applied Biology 132 20 21
supplement Tests of Agrochemicals and Cultivars No. 19)

Nasir M Hoppe HH Ebrahim Nesbat F 1992 The development of different pathotype groups of Mycosphaerella pinodes in susceptible and partially resistant pea leaves. Plant Pathology 41 187 194


Neergaard P (1979) ‘Seed pathology.’ Vols I & II. (Macmillan Press Ltd.: London)

Ogle HJ (1997) Disease management: chemicals. In ‘Plant pathogens and plant diseases’. (Eds JF Brown, HJ Ogle) pp. 373–389. (Rockvale Publications: Armidale)

Oku H Shiraishi T Ouchi S 1986 Specificity of local resistance induced in pea leaves by elicitor isolated from Mycosphaerella pinodes. Annals of the Phytopathological Society of Japan 52 347 348


Onfroy C Tivoli B Corbiere R Bouznad Z 1999 Cultural, molecular and pathogenic variability of Mycosphaerella pinodes and Phoma medicaginis var. pinodella isolates from dried pea (Pisum sativum) in France. Plant Pathology 48 218 229
doi:10.1046/j.1365-3059.1999.00323.x

Peck DM , McDonald GK (2001) Survey of field pea production practices in South Australia. In ‘10th Australian Agronomy Conference’. Hobart, Tasmania. p. 147.

Peck DM , McDonald GK , Davidson JA (2001) Blackspot survival in soil and stubble and aerial dissemination through the season. In ‘10th Australian Agronomy Conference’. Hobart, Tasmania. (Australian Society of Agronomy)

Platero Sanz M Fuchs A 1978 Degradation of pisatin, an antimicrobial compound produced by Pisum sativum L. Phytopathologia Mediterranea 17 14 17

Platero Sanz M Fuchs A 1980 Short-lived protection of pea plants against Mycosphaerella pinodes by prior inoculation with Pseudomonas phaseolicola. Netherlands Journal of Plant Pathology 86 181 190
doi:10.1007/BF01989710

Porta Puglia A , Bretag TW , Brouwer JB , Haware MP , Khalil SA (2000) Direct and indirect influences of morphological variations on diseases, yield and quality. In ‘Linking Research and Marketing Opportunities for Pulses in the 21st Century’. (Ed. R Knight) pp. 199–220. (Kluwer Academic Publishers: Dordrecht, The Netherlands)

Prioul S Frankewitz A Deniot G Morin G Baranger A 2004 Mapping of quantitative trait loci for partial resistance to Mycosphaerella pinodes in pea (Pisum sativum L.), at the seedling and adult plant stages. Theoretical and Applied Genetics 108 1322 1334 doi:10.1007/s00122-003-1543-2

Prioul S Onfroy C Tivoli B Baranger A 2003 Controlled environment assessment of partial resistance to Mycosphaerella pinodes in pea (Pisum sativum L.) seedlings. Euphytica 131 121 130 doi:10.1023/A:1023072214685

Punithalingam E Gibson IAS 1976 Phoma medicaginis var. pinodella. C.M.I. Descriptions of Pathogenic Fungi and Bacteria 518 1 2

Punithalingam E Holliday P 1972 a Ascochyta pisi. C.M.I. Descriptions of Pathogenic Fungi and Bacteria 334 1 2


Punithalingam E Holliday P 1972 b Mycosphaerella pinodes. C.M.I. Descriptions of Pathogenic Fungi and Bacteria 340 1 2


Rattan PS 1974 Decomposition of cellulose by three pea pathogens in pure culture. Transactions of the British Mycological Society 62 113 117


Rennie RJ Howard RJ Swanson TA Flores GHA 1985 The effect of seed applied pesticides on growth and nitrogen fixation in pea, lentil, and fababean. Canadian Journal of Plant Science 65 23 28


Roger C Tivoli B 1996 Spatio-temporal development of pycnidia and perithecia and dissemination of spores of Mycosphaerella pinodes on pea (Pisum sativum). Plant Pathology 45 518 528
doi:10.1046/j.1365-3059.1996.d01-139.x

Roger C Tivoli B Huber L 1999 a Effects of interrupted wet periods and different temperatures on the development of ascochyta blight caused by Mycosphaerella pinodes on pea (Pisum sativum) seedlings. Plant Pathology 48 10 18 doi:10.1046/j.1365-3059.1999.00311.x

Roger C Tivoli B Huber L 1999 b Effects of temperature and moisture on disease and fruit body development of Mycosphaerella pinodes on pea (Pisum sativum). Plant Pathology 48 1 9 doi:10.1046/j.1365-3059.1999.00312.x

Roger C , Tivoli B , Lemarchand E (1998 a) Epidemiological studies of ascochyta blight (Mycosphaerella pinodes) development on pea: understanding and predicting epidemics. In ‘3rd European Conference on Grain Legumes: Healthy and Added-value Crops to meet European Demands’. Valladolid, Spain. (Ed. AEP) pp. 100–101.

Roger C Tivoli B Lemarchand E 1998 b L’Anthracnose du pois proteagineux. Phytoma 509 32 36

Sakar D Muehlbauer FJ Kraft JM 1982 Techniques of screening peas for resistance to Phoma medicaginis var. pinodella. Crop Science 22 988 992


Salam MU , Galloway J , ‘Blackspot Manager’ for understanding blackspot of peas and ascochyta blight management. In ‘2005 Lupin and Pulses Updates. Agribusiness Crop Updates’. pp. 67–69. (Dept. of Agriculture, Western Australia: South Perth, WA)

Salter WJ , Smith JM (1986) Peas – Control of establishment pests and diseases using metalaxyl based seed coatings. In ‘1986 British Plant Protection Conference – Pests and diseases’. Brighton, England. pp. 1093–1100.

Sattar A 1934 A comparative study of the fungi associated with blight diseases of certain cultivated leguminous plants. Transactions of the British Mycological Society 18 276 301


Sharma B Tyagi MC 1977 Effect of seed quality on flowering time in pea (Pisum sativum). Seed Research 5 73 77


Sheridan JJ Dickinson CH 1968 A comparison of the behaviour of two pea pathogens in cut-away and loam. Irish Journal of Agricultural Research 7 335 342


Shiraishi T Oku H Tsuji Y Ouchi S 1978 a Inhibitory effect of pisatin on infection process of Mycosphaerella pinodes on pea. Annals of the Phytopathological Society of Japan 44 641 645


Shiraishi T Oku H Yamashita M Ouchi S 1978 b Elicitor and suppressor of pisatin induction in spore germination fluid of pea pathogens, Mycosphaerella pinodes. Annals of the Phytopathological Society of Japan 44 659 665


Shiraishi T , Ouchi S , Oku H (1983) Regulation of resistance expression in Pisum sativum by elicitor and suppressor of Mycosphaerella pinodes. In ‘4th International Congress of Plant Pathology’. Melbourne, Australia p. 194.

Skolko AJ Groves JW Wallen VR 1954 Ascochyta diseases of peas in Canada with special reference to seed transmission. Canadian Journal of Agricultural Science 34 417 427


Snyder WC Hansen HN 1947 Advantages of natural media and environments in the culture of fungi. Phytopathology 37 420 421


Sprague R 1929 Host range and life history studies of some leguminous ascochytae. Phytopathology 19 917 932


Stone RE 1912 The life history of Ascochyta on some leguminous plants. Annals of Mycology 10 564 592


Tar’an B Warkentin T Somers DJ Miranda D Vandenberg A Blade S Woods S Bing D Xue A DeKoeyer D Penner G 2003 Quantitative trait loci for lodging resistance, plant height and partial resistance to mycosphaerella blight in field pea (Pisum sativum L.). Theoretical and Applied Genetics 107 1482 1491
doi:10.1007/s00122-003-1379-9

Thomas JE , Kenyon DM , Biddle AJ , Ward RL (2000) Forecasting and control of leaf and pod spot (Mycosphaerella pinodes) on field peas. In ‘The British Crop Protection Council Conference: Pests and Diseases’. Brighton, UK. pp. 871–876.

Timmerman-Vaughan GM Frew TJ Butler R Murray S Gilpin M Falloon K Johnston P Lakeman MB Russell A Khan T 2004 Validation of quantitative trait loci for Ascochyta blight resistance in pea (Pisum sativum L.), using populations from two crosses. Theoretical and Applied Genetics 109 1620 1631 doi:10.1007/s00122-004-1779-5

Timmerman-Vaughan GM Frew TJ Russell AC Khan T Butler R Gilpin M Murray S Falloon K 2002 QTL mapping of partial resistance to field epidemics of Ascochyta blight of pea. Crop Science 42 2100 2111

Tivoli B Beasse C Lemarchand E Masson E 1996 Effect of Ascochyta blight (Mycosphaerella pinodes) on yield components of single pea (Pisum sativum) plants under field conditions. Annals of Applied Biology 129 207 216


Tivoli B Ney B Jeuffroy MH 1998 Towards a greater awareness of the impact of Ascochyta blight in fungicidal pea crop control. Phytoma 512 16 20


Tohamy AM Nour Eldin HA Mohamed ZK Gad El-Karim GA Madkour MA 1997 Identification of three Ascochyta spp. by random amplified polymorphic DNA. Phytopathology 87 S97


Uehara K 1964 Relationship between the host specificity of pathogen and phytoalexin. Annals of the Phytopathological Society of Japan 29 103 110


Van der Plank JE (1968) ‘Disease resistance in plants.’ (Academic Press: New York)

Wade GC 1951 Pea diseases in Tasmania. Journal of Agriculture, Tasmania 22 40 49


Walker J 1961 Mycosphaerella blight of peas. Agricultural Gazette, New South Wales 72 192 194


Walker JC (1969) Diseases incited by ascomycetes. V. Mycosphaerella and Ascochyta diseases of pea. In ‘Plant pathology’. (Ed. JC Walker) pp. 369–376. (McGraw-Hill, Inc.: New York)

Walker JC Hare WW 1943 Pea diseases in Wisconsin in 1942. Wisconsin Agricultural Experimental Station Research Bulletin 145 1 33


Wallen VR 1955 The effect of storage for several years on the viability of Ascochyta pisi in pea seed and on the germination of the seed and emergence. Plant Disease Reporter 39 674 677


Wallen VR 1957 The identification and distribution of physiologic races of Ascochyta pisi Lib. in Canada. Canadian Journal of Plant Science 37 337 341


Wallen VR 1965 Field evaluation and the importance of the Ascochyta complex on peas. Canadian Journal of Plant Science 45 27 33


Wallen VR 1974 Influence of three Ascochyta diseases of peas on plant development and yield. Canadian Plant Disease Survey 54 86 90


Wallen VR Cuddy TF 1968 Ascochyta blight of field peas. Canadian Agriculture 13 24 25


Wallen VR Cuddy TF Grainger PN 1967 Epidemiology and control of Ascochyta pinodes on field peas in Canada. Canadian Journal of Plant Science 47 395 401


Wallen VR Hoffman I 1959 Fungistatic activity of captan in pea seedlings after treatment of the seeds or roots of seedlings. Phytopathology 49 680 683


Wallen VR Jeun J 1968 Factors limiting the survival of Ascochyta spp. of peas in soil. Canadian Journal of Botany 46 1279 1286


Wang H Hwang SF Chang KF Turnbull GD Howard RJ 2000 Characterization of Ascochyta isolates and susceptibility of pea cultivars to the ascochyta disease complex in Alberta. Plant Pathology 49 540 545
doi:10.1046/j.1365-3059.2000.00489.x

Wark DC 1950 a The inheritance of resistance to Ascochyta pisi Lib. in Pisum sativum L. Australian Journal of Agricultural Research 1 382 390 doi:10.1071/AR9500382

Wark DC 1950 b The susceptibility of peas to four diseases occurring naturally in the Australian Capital Territory. The Journal of the Australian Institute of Agricultural Science 16 32 33

Warkentin TD Rashid KY Xue AG 1996 Fungicidal control of Ascochyta blight of field pea. Canadian Journal of Plant Science 76 67 71


Warkentin TD Xue AG McAndrew DW 2000 Effect of mancozeb on the control of Mycosphaerella blight of field pea. Canadian Journal of Plant Science 80 403 406


Wroth JM (1996) Host-pathogen relationships of the ascochyta blight (Mycosphaerella pinodes (Berk. & Blox.) Vestegr.) disease of field pea (Pisum sativum L.). PhD thesis, University of Western Australia, Australia.

Wroth JM 1998 a Possible role for wild genotypes of Pisum spp. to enhance ascochyta blight resistance in pea. Australian Journal of Experimental Agriculture 38 469 479
doi:10.1071/EA98024

Wroth JM 1998 b Variation in pathogenicity among and within Mycosphaerella pinodes populations collected from field pea in Australia. Canadian Journal of Botany 76 1955 1966 doi:10.1139/cjb-76-11-1955

Wroth JM 1999 Evidence suggests that Mycosphaerella pinodes infection of Pisum sativum is inherited as a quantitative trait. Euphytica 107 193 204 doi:10.1023/A:1003688430893

Xue AG 2000 Effect of seed-borne Mycosphaerella pinodes and seed treatments on emergence, foot rot severity, and yield of field pea. Canadian Journal of Plant Pathology 22 248 253

Xue AG Warkentin TD 2001 Partial resistance to Mycosphaerella pinodes in field pea. Canadian Journal of Plant Science 81 535 540


Xue AG Warkentin TD Gossen BD Burnett PA Vandenberg A Rashid KY 1998 Pathogenic variation of western Canadian isolates of Mycosphaerella pinodes on selected Pisum genotypes. Canadian Journal of Plant Pathology 20 189 193


Xue AG Warkentin TD Greeniaus MT Zimmer RC 1996 Genotypic variability in seedborne infection of field pea by Mycosphaerella pinodes and its relation to foliar disease severity. Canadian Journal of Plant Pathology 18 370 374


Xue AG Warkentin TD Kenaschuk EO 1997 Effects of timings of inoculation with Mycosphaerella pinodes on yield and seed infection of field pea. Canadian Journal of Plant Science 77 685 689


Zhang JX Fernando WGD Xue AG 2003 Virulence and genetic variability among isolates of Mycosphaerella pinodes. Plant Disease 87 1376 1383


Zhang JX Fernando WGD Xue AG 2004 Temporal and spatial dynamics of mycosphaerella blight. Canadian Journal of Plant Pathology 26 522 532


Zhang JX Fernando WGD Xue AG 2005 a Daily and seasonal spore dispersal by Mycosphaerella pinodes and development of mycosphaerella blight of field pea. Canadian Journal of Botany 83 302 310
doi:10.1139/b05-003

Zhang JX Xue AG Fernando WGD 2005 b Effect of residue type and burial depth on survival of Mycosphaerella pinodes in Manitoba. Canadian Journal of Plant Pathology 27 132 136

Zimmer RC Sabourin D 1986 Determining resistance reactions of field pea cultivars at the seedling stage to Mycosphaerella pinodes. Phytopathology 76 878 881




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