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Plant sciences, sustainable farming systems and food quality
RESEARCH ARTICLE

Widespread occurrence of multiple herbicide resistance in Western Australian annual ryegrass (Lolium rigidum) populations

Mechelle J. Owen A C , Michael J. Walsh A , Rick S. Llewellyn A B and Stephen B. Powles A
+ Author Affiliations
- Author Affiliations

A Western Australian Herbicide Resistance Initiative, School of Plant Biology, University of Western Australia, 35 Stirling Hwy, Crawley, WA 6009, Australia.

B Current address: CSIRO Sustainable Ecosystems, Urrbrae, Post: PMB 2 Glen Osmond, SA 5064, Australia.

C Corresponding author. Email: mowen@cyllene.uwa.edu.au

Australian Journal of Agricultural Research 58(7) 711-718 https://doi.org/10.1071/AR06283
Submitted: 25 August 2006  Accepted: 28 March 2007   Published: 26 July 2007

Abstract

In 2003, a random survey was conducted across the Western Australian wheatbelt to establish the frequency and distribution of herbicide resistance in ryegrass populations infesting crop fields. Five hundred cropping fields were visited at crop maturity, and ryegrass seed was collected in 452 of these fields. Subsequently, each crop field population was screened with herbicides of various modes of action that are commonly used for ryegrass control in Australian cropping systems. Most of these ryegrass populations were found to be resistant to the ACCase-inhibitor herbicide diclofop-methyl (68%) and the ALS-inhibitor herbicide sulfometuron (88%). A comparison of resistance levels in the same agronomic zones surveyed 5 years earlier determined that there had been an increase of 20 percentage points in the frequency of resistance over this 5-year period. This survey also determined that the majority (64%) of populations were found to be multiple resistant to both diclofop-methyl and sulfometuron. The distribution patterns of the collected populations indicated that there were higher frequencies of resistant and developing resistance populations occurring in the intensively cropped regions of the wheatbelt, which had greater herbicide selection pressure. Of concern is that 24% and 8% of populations were found to be developing resistance to trifluralin and clethodim, respectively. Currently these herbicides are heavily relied upon for control of ACCase and ALS herbicide resistant ryegrass. Nearly all populations remain susceptible to glyphosate. Ryegrass across the WA wheatbelt now exhibits multiple resistance across many but not all herbicides, posing severe management and sustainability challenges.

Additional keywords: resistance survey, resistance evolution.


Acknowledgments

This project was funded by the Grains Research and Development Corporation. We thank the members of the WAHRI research team (staff and students) who assisted in this field survey, Bolette Palle for invaluable technical assistance, and Frank D’Emden for assistance with data analysis.


References


Beckie HJ, Hall LM, Meers S, Laslo JJ, Stevenson FC (2004) Management practices influencing herbicide resistance in wild oat. Weed Technology 18, 853–859.
CrossRef |

Beckie HJ, Thomas AG, Legere A, Kelner DJ, Van Acker RC, Meers S (1999) Nature, occurrence, and cost of herbicide-resistant wild oat (Avena fatua) in small-grain production areas. Weed Technology 13, 612–625.

Broster JC, Pratley JE (2006) A decade of monitoring herbicide resistance in Lolium rigidum in Australia. Australian Journal of Experimental Agriculture 46, 1151–1160.
CrossRef |

Burnet MWM, Christopher JT, Holtum JAM, Powles SB (1994b) Identification of two mechanisms of resistance to ALS inhibiting herbicides using a selective germination medium. Weed Science 42, 468–473.

Burnet MWM, Hildebrand OB, Holtum JAM, Powles SB (1991) Amitrole, triazine, substituted urea and metribuzin resistance in a biotype of rigid ryegrass (Lolium rigidum). Weed Science 39, 317–323.

Burnet MWM, Holtum JAM, Powles SB (1994a) Resistance to nine herbicide classes in a Lolium rigidum biotype. Weed Science 42, 369–377.

Christopher JT, Powles SB, Holtum JAM (1992) Resistance to acetolactate synthase inhibiting herbicides in annual ryegrass (Lolium rigidum) involves at least two mechanisms. Plant Physiology 100, 1909–1913.
PubMed |


Delye C (2005) Weed resistance to acetyl-coenzyme A carboxylase inhibitors: an update. Weed Science 53, 728–746.
CrossRef |

Delye C, Zhang X, Chalopin C, Michel S, Powles SB (2003) An isoleucine residue within the carboxyl-transferase domain of multidomain acetyl-Coenzyme A carboxylase is a major determinant of sensitivity to aryloxyphenoxypropionate inhibitors but not to cyclohexanedione inhibitors. Plant Physiology 132, 1716–1723.
CrossRef | PubMed |

Gill GS (1995) Development of herbicide resistance in annual ryegrass populations (Lolium rigidum Gaud.) in the cropping belt of Western Australia. Australian Journal of Experimental Agriculture 35, 67–72.
CrossRef |

Hall LM , Holtum JAM , Powles SB (1994) Mechanisms responsible for cross resistance and multiple resistance. In ‘Herbicide resistance in plants: biology and biochemistry’. (Eds SB Powles, JAM Holtum) pp. 243–261. (CRC Press Inc.: Boca Raton, FL)

Heap I (2006) International survey of herbicide resistant weeds. Available online: www.weedscience.com

Heap I, Knight R (1982) A population of ryegrass tolerant to the herbicide diclofop-methyl. Journal of the Australian Institute of Agricultural Science 48, 156–157.

Heap I, Knight R (1986) The occurrence of herbicide cross resistance in a population of annual ryegrass, Lolium rigidum, resistant to diclofop-methyl. Australian Journal of Agricultural Research 37, 149–156.
CrossRef |

Holtum JAM, Matthews JM, Liljegren DR, Powles SB (1992) Cross-resistance to herbicides in annual ryegrass (Lolium rigidum). III. On the mechanism, of resistance to diclofop-methyl. Plant Physiology 97, 1026–1034.

Legere A, Beckie HJ, Stevenson FC, Thomas AG (2000) Survey of management practices affecting the occurrence of wild oat (Avena fatua) resistance to acetyl-CoA carboxylase inhibitors. Weed Technology 14, 366–376.
CrossRef |

Llewellyn RS, Allen DM (2006) Expected mobility of herbicide resistance via weed seeds and pollen in a Western Australian cropping region. Crop Protection 25, 520–526.
CrossRef |

Llewellyn RS, Powles SB (2001) High levels of herbicide resistance in rigid ryegrass (Lolium rigidum) in the wheat belt of Western Australia. Weed Technology 15, 242–248.
CrossRef |

Matthews JM, Holtum JAM, Liljegren DR, Furness B, Powles SB (1991) Cross-resistance to herbicides in annual ryegrass (Lolium rigidum). 1. Properties of the herbicide target enzymes acetyl-CoA carboxylase (ACC) and acetolactate synthase (ALS). Plant Physiology 94, 1180–1186.

McAlister FM, Holtum JAM, Powles SB (1995) Dinitroaniline herbicide resistance in rigid ryegrass (Lolium rigidum). Weed Science 43, 55–62.

Meddis R (1984) ‘Statistics using ranks.’ (Basil Blackwell Publisher Ltd: Oxford, UK)

Menchari Y, Camilleri C, Michel S, Brunel D, Dessaint F, Le Corre V, Delye C (2006) Weed response to herbicides: regional-scale distribution of herbicide resistance alleles in the grass weed Alopecurus myosuroides. New Phytolologist 171, 861–874.
CrossRef |

Neve PB, Saldler J, Powles SB (2004) Multiple herbicide resistance in a glyphosate-resistant (Lolium rigidum) biotype. Weed Science 52, 920–928.
CrossRef |

Powles SB, Lorraine-Colwill DF, Dellow JJ, Preston C (1998) Evolved resistance to glyphosate in rigid ryegrass (Lolium rigidum) in Australia. Weed Science 46, 604–607.

Powles SB, Preston C (2006) Evolved glyphosate resistance in plants: biochemical and genetic basis of resistance. Weed Technology 20, 282–289.
CrossRef |

Preston C (2005) ‘Australian glyphosate resistance register.’ (National Glyphosate Sustainability Working Group, www.weeds.crc.org.au/glyphosate)

Preston C, Powles SB (1998) Amitrole inhibits diclofop metabolism and synergises diclofop-methyl in a diclofop-methyl-resistant biotype of Lolium rigidum. Pesticide Biochemistry and Physiology 62, 179–189.
CrossRef |

Preston C , Powles SB (2002 a) Mechanisms of multiple herbicide resistance in Lolium rigidum. In ‘Agrochemical resistance: extent, mechanism and detection’. (Eds JM Clark, I Yamaguchi) pp. 150–160. (Oxford University Press: Oxford, UK)

Preston C, Powles SB (2002b) Evolution of herbicide resistance in weeds: initial frequency of target site-based resistance to acetolactate-synthase inhibiting herbicides in Lolium rigidum. Heredity 88, 8–13.
CrossRef | PubMed |

Preston C, Tardif FJ, Christopher JT, Powles SB (1996) Multiple resistance to dissimilar herbicide chemistries in a biotype of Lolium rigidum due to enhanced activity of several herbicide degrading enzymes. Pesticide Biochemistry and Physiology 54, 123–134.
CrossRef |

Saari LL , Cotterman JC , Thill DC (1994) Resistance to acetolactate synthase inhibiting herbicides. In ‘Herbicide resistance in plants: biology and biochemistry’. (Eds SB Powles, JAM Holtum) pp. 83–139. (CRC Press Inc.: Boca Raton, FL)

Steadman KJ, Bignell GP, Ellery AJ (2003) Field assessment of thermal after-ripening time for dormancy release prediction in Lolium rigidum seeds. Weed Research 43, 458–465.
CrossRef |

Tardif FJ, Holtum JAM, Powles SB (1993) Occurrence of a herbicide resistant acetyl-coenzyme A carboxylase mutant in annual ryegrass (Lolium rigidum) selected by sethoxydim. Planta 190, 176–181.
CrossRef |

Tardif FJ, Powles SB (1994) Herbicide multiple-resistance in a Lolium rigidum biotype is endowed by multiple mechanisms: Isolation of a subset with resistant acetyl-CoA carboxylase. Physiologia Plantarum 91, 488–494.
CrossRef |

Tardif FJ, Preston C, Holtum JAM, Powles SB (1996) Resistance to acetyl-coenzyme A carboxylase inhibiting herbicides endowed by a single major gene encoding a resistant target site in a biotype of Lolium rigidum. Australian Journal of Plant Physiology 23, 15–23.

Tranel PJ, Wright TR (2002) Resistance of weeds to ALS-inhibiting herbicides: what have we learned? Weed Science 50, 700–712.
CrossRef |

Zhang X, Powles SB (2006a) The molecular basis for resistance to acetyl co-enzyme A carboxylase (ACCase) inhibiting herbicides in two target-based resistant biotypes of annual ryegrass (Lolium rigidum). Planta 223, 550–557.
CrossRef | PubMed |

Zhang X, Powles SB (2006b) Six amino acid substitutions of the plastidic ACCase gene endow resistance to aryloxyphenoxypropionate and cyclohexanedione herbicides in a Lolium rigidum population. New Phytologist 172, 636–645.
CrossRef | PubMed |








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