Mapping quantitative trait loci for resistance against Russian wheat aphid (Diuraphis noxia) in wheat (Triticum aestivum L.)M. Ricciardi A , E. Tocho B , M. S. Tacaliti A , A. Vasicek A , D. O. Giménez A , A. Paglione A , J. Simmonds C , J. W. Snape C , M. Cakir D and A. M. Castro A B E
A Genetics, Plant Physiology and Entomology, Department of Plant Sciences, Faculty of Agriculture, Science, University of La Plata, CC31, 1900-La Plata, Argentina.
B National Council of Scientific Research, CONICET, CC31, 1900-La Plata, Argentina.
C John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom.
D WA State Agricultural Biotechnology Centre, Faculty of Sustainability, Environmental and Life Sciences, Murdoch University, Murdoch, WA 6150, Australia.
E Corresponding author. Email: firstname.lastname@example.org
Crop and Pasture Science 61(12) 970-977 https://doi.org/10.1071/CP10188
Submitted: 30 May 2010 Accepted: 18 October 2010 Published: 8 December 2010
Diuraphis noxia (Russian wheat aphid, RWA), one of the most aggressive pests of wheat, has evolved several biotypes with virulence matching known Dn resistance genes. This paper was aimed at determining the location of plant-defence genes triggered by RWA in a set of doubled haploid (DH) lines obtained from the cross of winter wheat varieties ‘Spark’ and ‘Rialto’. Both parental lines, 110 DH and CItr2401 (a RWA-resistant line) were screened for antixenosis, tolerance and antibiotic mechanisms of resistance with a population of RWA collected in Argentina. Antixenosis was not significantly linked to any marker locus. Tolerance traits showed significant associations with several chromosomes. Quantitative trait loci (QTL) for the foliar area developed during infestation was significantly associated with marker loci Xpsp3103 on 4DS, and Xgdm3 on 5DS. QTL for chlorophyll content in the infested plants were significantly associated with the marker loci Xgwm533 on 3BS and Xpsp3094 on 7AL, and a QTL for the number of expanded leaves was associated with the marker loci Xwmc264 on 3AS and XwPt8836 on 4DS. QTL for most of the tolerance traits were significantly associated with the same chromosome intervals on chromosomes 4DS and 5DS. The 4DS QTL were linked to or had a pleiotropic effect on Rht-D1. Most of the antibiosis traits were significantly associated with the same marker loci on chromosomes 4A (XwPt7405), 1B (XwPt9032) and 5B (Xbarc109 and Xbarc74). Several novel genes conferring tolerance and antibiosis to RWA were identified and these could be transferred into wheat cultivars to enlarge the genetic base of defence against this aphid pest. These new genes can be designated as QDn.unlp genes, following the rules for gene nomenclature in wheat.
Additional keywords: antibiosis, antixenosis, DArT markers, marker-assisted selection, tolerance.
ReferencesAgrawal G, Rakwal R, Jwa N, Agrawal V (2002) Characterization of a novel rice gene and modulation of its expression by components of the stress signalling pathways. Physiologia Plantarum 116, 87–95.
Baldwin IT, Preston CJ (1999) The ecophysiological complexity of plant responses to herbivores. Planta 208, 137–145.
Bassam BJ, Caetano-Anolles G, Gresshoff PM (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Analytical Biochemistry 196, 80–83.
Boyko E, Starkey S, Smith M (2004) Molecular genetic mapping of Gby, a new greenbug resistance gene in bread wheat. Theoretical and Applied Genetics 109, 1230–1236.
| Molecular genetic mapping of Gby, a new greenbug resistance gene in bread wheat.CrossRef | 15309299PubMed |
Burd J, Porter D, Puterka G, Haley S, Peairs F (2006) Biotypic variation among North American Russian wheat aphid (Homoptera: Aphididae) populations. Journal of Economic Entomology 99, 1862–1866.
Campbell BT, Baenziger PS, Gill KS, Eskridge KM, Budak H, Erayman M, Dweikat I, Yen Y (2003) Identification of QTLs and environmental interactions associated with agronomic traits on chromosome 3A of wheat. Crop Science 43, 1493–1505. http://crop.scijournals.org/cgi/content/abstract/43/4/1493
Castro AM, Vasicek A, Manifiesto M, Giménez DO, Tacaliti MS, Dobrovolskaya O, Röder MS, Snape JW, Börner A (2005) Mapping antixenosis genes on chromosome 6A of wheat to greenbug and to a new biotype of Russian wheat aphid. Plant Breeding 124, 229–233.
Castro AM, Worland AJ, Vasicek A, Ellerbrook C, Giménez DO, Tocho E, Tacaliti MS, Clúa A, Snape JW (2004) Mapping quantitative trait loci for resistance against greenbug and Russian wheat aphid. Plant Breeding 123, 361–366.
Collins MB, Haley SD, Peairs FB, Rudolph JB (2005) Biotype 2 Russian Wheat aphid resistance among wheat germplasm accessions. Crop Science 45, 1877–1880.
Deol GS, Reese JC, Gill BS, Wilde GE, Campbell LR (2001) Comparative chlorophyll losses in susceptible wheat leaves fed upon by Russian wheat aphids or greenbugs (Homoptera: Aphididae). Journal of the Kansas Entomological Society 74, 192–198.
Du Toit F (1987) Resistance in wheat (Triticum aestivum) to Diuraphis noxia (Homoptera: Aphididae). Cereal Research Communications 15, 175–179.
Ellerbrook C, Korzun V, Worland AJ (1999) Using precise genetic stocks to investigate the control of Stagnospora nodorum resistance in wheat. In ‘Septoria and Stagnospora nodorum diseases of cereals: a compilation of global research’. (Eds M van Ginkel, A McNab, J Krupinsky) pp. 134–139. (CIMMYT: Mexico, DF) Available at: www.cimmyt.org/Research/Wheat/pdf/septoria_ago99.pdf
Ennahli S, El Bouhssini M, Grando S, Anathakrishnan R, Niide T, Starkus L, Starkey S, Smith CM (2009) Comparison of categories of resistance in wheat and barley genotypes against biotype 2 of the Russian wheat aphid Diuraphis noxia (Kurdjumov). Arthropod–Plant Interactions 3, 45–53.
Haley SD, Peairs FB, Walker GE, Rudolph JB, Randolph TL (2004) Occurrence of a new Russian wheat aphid biotype in Colorado. Crop Science 44, 1589–1592. Available at: http://crop.scijournals.org/cgi/reprint/44/5/1589
Hoagland DR, Arnon DI (1959) The water culture method for growing plants without soil. California Agriculture Experimental Station Circular 347, 1–32.
Jaccoud D, Peng K, Feinstein D, Kilian A (2001) Diversity arrays: a solid state technology for sequence information independent genotyping. Nucleic Acids Research 29, e25
| Diversity arrays: a solid state technology for sequence information independent genotyping.CrossRef | 11160945PubMed |
Jyoti JL, Michaud JP (2005) Comparative biology of a novel strain of Russian wheat aphid (Homoptera: Aphididae) on three wheat cultivars. Journal of Economic Entomology 98, 1032–1039.
Jyoti JL, Qureshi JA, Michaud JP, Martin TJ (2006) Virulence of two Russian wheat aphid biotypes to eight wheat cultivars at two temperatures. Journal of Economic Entomology 99, 1214–1224.
Karban R, Baldwin IT (1997) ‘Induced responses to herbivory.’ (The University of Chicago Press: Chicago, IL)
Kuchel H, Williams K, Langridge P, Eagles HA, Jefferies SP (2007) Genetic dissection of grain yield in bread wheat. II. QTL-by-environment interaction. Theoretical and Applied Genetics 115, 1015–1027.
Liu XM, Smith CM, Gill BS (2002) Identification of microsatellite markers linked to Russian wheat aphid resistance genes Dn4 and Dn6. Theoretical and Applied Genetics 104, 1042–1048.
Liu XM, Smith CM, Gill BS (2005) Allelic relationships among Russian wheat aphid resistance genes. Crop Science 45, 2273–2280.
Liu XM, Smith CM, Gill BS, Tolmay V (2001) Microsatellite markers linked to six Russian wheat aphid resistance genes in wheat. Theoretical and Applied Genetics 102, 504–510.
Marais GF, Horn M, Du Toit F (1994) Intergeneric transfer (rye to wheat) of a gene(s) for Russian wheat aphid resistance. Plant Breeding 113, 265–271.
McIntosh RA, Yamazaki Y, Devos K, Dubcovsky J, Rogers J, Appels R (2003) Catalogue of gene symbols for wheat. Available at: www.grs.nig.ac.jp/wheat/komugi/genes
Navarro L, Bari R, Achard P, Lison P, Nemri A, Harberd NP, Jones JD (2008) DELLAs control plant immune responses by modulating the balance of jasmonic acid and salicylic acid signaling. Current Biology 18, 650–655.
Nkongolo KK, Quick JS, Meyer WL, Peairs FB (1991) Sources and inheritance of Russian wheat aphid (Diuraphis noxia, Mordv.) resistance in Triticum species, amphiploids and Triticum tauschii. Canadian Journal of Plant Science 71, 703–708.
Plaschke J, Ganal MW, Röder MS (1995) Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theoretical and Applied Genetics 91, 1001–1007.
Porter DR, Baker CA, El-Bouhssini M (2005) Resistance in wheat to a new North American Russian wheat aphid biotype. Plant Breeding 124, 603–604.
Qureshi JA, Jyoti JL, Michaud JP (2005) Differential colonization of wheat cultivars by two biotypes of Russian wheat aphid (Homoptera: Aphididae). Insect Science 12, 341–349.
Ricci M, Ortego J, Castro AM (2006) Variability of Diuraphis noxia Kurdjumov (Hemiptera: Aphididae) reproductive behaviour in wheat. Basic and Applied Genetics 35, 155
SAS (1998) ‘SAS/STAT guide for personal computers. Version 6.03.’ (SAS Institute: Cary, NC)
Seaton G, Haley C, Knott S, Kearsey M, Visscher P (2002) QTL express: mapping quantitative trait loci in simple and complex pedigrees. Bioinformatics 18, 339–340. Available at: http://qtl.cap.ed.ac.uk
Smith CM (1999) Plant resistance to insects. In ‘Biological and biotechnological control of insects’. (Eds J Rechcigl, N Rechcigl) pp. 171–207. (Lewis: Boca Raton, FL)
Smith CM, Belay T, Satuffer C, Stary P, Kubeckova I, Starkey S (2004) Identification of Russian wheat aphid (Homoptera: Aphididae) populations virulent to the Dn4 resistance gene. Journal of Economic Entomology 97, 1112–1117.
Smith CM, Boyko EV (2006) A functional genomic approach to identify temperature response genes modulating plant defense responses to arthropod challenge. In ‘Ecological genomics in Kansas. 4th Ecological Genomics Symposium’. 3–5 Nov. 2006, Kansas City. pp. 10–14. (Kansas State University: Kansas, KS)
Snape JW, Foulkes MJ, Simmonds J, Leverington M, Fish LJ, Wang Y, Ciavarrella M (2007) Dissecting gene X environmental effects on wheat yields via QTL and physiological analysis. Euphytica 154, 401–408.
Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics 109, 1105–1114.
Stam P (1993) Constructing of integrated genetic linkage maps by means of a new computer package: JOINMAP. The Plant Journal 3, 739–744.
Tolmay VL, Lindeque RC, Prinsloo GJ (2007) Preliminary evidence of a resistance-breaking biotype of the Russian wheat aphid, Diuraphis noxia (Kurdjumov) (Homoptera: Aphididae), in South Africa. African Entomology 15, 228–230.
Voothuluru P, Meng J, Khajuria C, Louis J, Zhu L, Starkey S, Wilde G, Baker C, Smith M (2006) Categories and inheritance of resistance to Russian wheat aphid (Homoptera: Aphididae) Biotype 2 in a selection from wheat cereal introduction 2401. Journal of Economic Entomology 99, 1854–1861.
Wenzl P, Carling J, Kudrna D, Jaccoud D, Huttner E, Kleinhofs A, Kilian A (2004) Diversity arrays technology (DArT) for whole-genome profiling of barley. Proceedings of the National Academy of Sciences of the United States of America 101, 9915–9920.
Yencho GC, Cohen MB, Byrne PF (2000) Applications of tagging and mapping insect resistance loci in plants. Annual Review of Entomology 45, 393–422. Available at: http://arjournals.annualreviews.org/doi/pdf/10.1146/annurev.ento.45.1.393
Zhang Y, Turner JG (2008) Wound-induced endogenous jasmonates stunt plant growth by inhibiting mitosis. PLoS ONE 3, e3699