Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality
RESEARCH ARTICLE

Introgression of allelic diversity from genetically distinct variants of Brassica rapa into Brassica napus canola and inheritance of the B. rapa alleles

Rohit Attri A and Habibur Rahman A B
+ Author Affiliations
- Author Affiliations

A Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada.

B Corresponding author. Email: habibur.rahman@ualberta.ca

Crop and Pasture Science 69(1) 94-106 https://doi.org/10.1071/CP17193
Submitted: 25 May 2017  Accepted: 8 October 2017   Published: 13 November 2017

Abstract

Broadening of genetic diversity in spring oilseed Brassica napus L. (AACC, 2n = 38) canola is important for continued improvement of this crop. For this, the vast allelic diversity of the A genome of Brassica rapa L. (AA, 2n = 20) can be utilised. We investigated the prospect of developing canola-quality euploid B. napus lines carrying the alleles of B. rapa from F2 and BC1 (F1 × B. napus) populations of three B. napus × B. rapa interspecific crosses involving one B. napus and three genetically distinct B. rapa parents. In meiosis, the F1 AAC hybrid was expected to show normal segregation for the A genome chromosomes, whereas a range of C chromosomes from zero to nine was expected to be included in the gametes due to random segregation of this haploid set of chromosomes. Subsequent self-pollination, theoretically, should have eliminated the unpaired C chromosomes and resulted in a majority of B. rapa type. However, no B. rapa-type progeny were detected, and all progeny in the F8 conformed to be B. napus type. Correlation between parent and offspring generation, grown in greenhouse or field, was weak to moderate for seed glucosinolate content; however, the simpler genetic control of this trait, involving only the A genome loci, allowed the development of low-glucosinolate lines from this interspecific cross. Of the theoretical number of simple sequence repeat (SSR) marker alleles of B. rapa expected to be present in F4 and F8 populations, about 45% were detected in these populations, suggesting that the loss of these marker alleles occurred prior to the F4 generation. Loss of several SSR loci was also detected in these populations, which probably resulted from homoeologous pairing and rearrangements of the chromosomes of the A and C genomes. Genetic diversity analysis performed on the F8 progeny of two crosses showed that the two populations clustered into distinct groups, which demonstrates that they inherited SSR B. rapa alleles unique to each B. rapa parent. We conclude that B. rapa alleles from diverse sources can be readily incorporated into B. napus progeny by this interspecific crossing method.

Additional keywords: marker heritance, chromosome stabilisation, fertility, flow cytometry, parent–offspring.


References

Annisa , Chen S, Cowling WA (2013) Global genetic diversity in oilseed Brassica rapa. Crop & Pasture Science 64, 993–1007.
Global genetic diversity in oilseed Brassica rapa.CrossRef |

Attia T, Röbbelen G (1986) Cytogenetic relationship within cultivated Brassica analyzed in amphihaploids from the three diploid ancestors. Canadian Journal of Genetics and Cytology 28, 323–329.
Cytogenetic relationship within cultivated Brassica analyzed in amphihaploids from the three diploid ancestors.CrossRef |

Attia T, Busso C, Röbbelen G (1987) Digenomic triploids for an assessment of chromosome relationships in the cultivated diploid Brassica species. Genome 29, 326–330.
Digenomic triploids for an assessment of chromosome relationships in the cultivated diploid Brassica species.CrossRef |

Attri R (2015) Broadening of genetic diversity in spring canola (Brassica napus L.) by use of yellow sarson and Canadian spring Brassica rapa L. MSc Thesis, University of Alberta, Edmonton, Canada.

Babula D, Kaczmarek M, Ziółkowski PA, Sadowski J (2007) Brassica oleracea. In ‘Genome mapping and molecular breeding in plants: Vegetables’. Vol. 5. (Ed. C Kole) pp. 227–285. (Springer-Verlag: Berlin)

Bahrani J, McVetty PBE (2008) Relationship of seed quality traits for greenhouse-grown versus field-grown high erucic acid rapeseed: Is seed quality trait selection for greenhouse-grown seed worthwhile? Canadian Journal of Plant Science 88, 419–423.
Relationship of seed quality traits for greenhouse-grown versus field-grown high erucic acid rapeseed: Is seed quality trait selection for greenhouse-grown seed worthwhile?CrossRef | 1:CAS:528:DC%2BD1cXosFKis7s%3D&md5=5171235427a841e3895397cc62b13040CAS |

Becker HC, Engqvist GM, Karlsson B (1995) Comparison of rapeseed cultivars and resynthesized lines based on allozyme and RFLP markers. Theoretical and Applied Genetics 91, 62–67.
Comparison of rapeseed cultivars and resynthesized lines based on allozyme and RFLP markers.CrossRef | 1:CAS:528:DyaK2MXnsFKrsbc%3D&md5=49b1c795dfc47af8ee39a84793ec39d7CAS |

Bennett RA, Thiagarajah MR, King JR, Rahman MH (2008) Interspecific cross of Brassica oleracea var. alboglabra and B. napus: effects of growth condition and silique age on the efficiency of hybrid production, and inheritance of erucic acid in the self-pollinated backcross generation. Euphytica 164, 593–601.
Interspecific cross of Brassica oleracea var. alboglabra and B. napus: effects of growth condition and silique age on the efficiency of hybrid production, and inheritance of erucic acid in the self-pollinated backcross generation.CrossRef | 1:CAS:528:DC%2BD1cXhtFOgtL3K&md5=afa24eba798ee0d7926f1750e3760572CAS |

Bennett RA, Séguin-Swartz G, Rahman H (2012) Broadening genetic diversity in canola using C-genome species Brassica oleracea L. Crop Science 52, 2030–2039.
Broadening genetic diversity in canola using C-genome species Brassica oleracea L.CrossRef |

Bing DJ, Downey RK, Rakow GFW (1996) Hybridizations among Brassica napus, B. rapa and B. juncea and their two weedy relatives B. nigra and Sinapis arvensis under open pollination conditions in the field. Plant Breeding 115, 470–473.
Hybridizations among Brassica napus, B. rapa and B. juncea and their two weedy relatives B. nigra and Sinapis arvensis under open pollination conditions in the field.CrossRef |

Brown J, Brown AP (1996) Gene transfer between canola (Brassica napus L. and B. campestris L.) and related weed species. Annals of Applied Biology 129, 513–522.
Gene transfer between canola (Brassica napus L. and B. campestris L.) and related weed species.CrossRef |

Bus A, Körber N, Snowdon RJ, Stich B (2011) Patterns of molecular variation in a species-wide germplasm set of Brassica napus. Theoretical and Applied Genetics 123, 1413–1423.
Patterns of molecular variation in a species-wide germplasm set of Brassica napus.CrossRef |

Canadian Food Inspection Agency 2008. Cruciferous crop inspection procedures: Appendix VII – Plant descriptions and illustrations of mustard and canola. Government of Canada. Available at: www.inspection.gc.ca/english/plaveg/seesem/man/swi-crue.shtml#a22 (accessed 30 July 2012).

Chen S, Nelson MN, Ghamkhar K, Fu T, Cowling WA (2008) Divergent patterns of allelic diversity from similar origins: the case of oilseed rape (Brassica napus L.) in China and Australia. Genome 51, 1–10.
Divergent patterns of allelic diversity from similar origins: the case of oilseed rape (Brassica napus L.) in China and Australia.CrossRef |

Cheng X, Xu J, Xia S, Gu J, Yang Y, Fu J, Qian X, Zhang S, Wu J, Kede L (2009) Development and genetic mapping of microsatellite markers from genome survey sequences in Brassica napus. Theoretical and Applied Genetics 118, 1121–1131.
Development and genetic mapping of microsatellite markers from genome survey sequences in Brassica napus.CrossRef | 1:CAS:528:DC%2BD1MXktFekt74%3D&md5=b502e285900a4c77449ea76313503a0eCAS |

Chiang MS, Chiang BY, Grant WF (1977) Transfer of resistance to race 2 of Plasmodiophora brassicae from Brassica napus to cabbage (B. oleracea var. capitata). I. Interspecific hybridization between B. napus and B. oleracea var. capitata. Euphytica 26, 319–336.
Transfer of resistance to race 2 of Plasmodiophora brassicae from Brassica napus to cabbage (B. oleracea var. capitata). I. Interspecific hybridization between B. napus and B. oleracea var. capitata.CrossRef |

Cowling WA (2007) Genetic diversity in Australian canola and implications for crop breeding for changing future environments. Field Crops Research 104, 103–111.
Genetic diversity in Australian canola and implications for crop breeding for changing future environments.CrossRef |

Diers BW, Osborn TC (1994) Genetic diversity of oilseed Brassica napus germplasm based on restriction fragment length polymorphisms. Theoretical and Applied Genetics 88, 662–668.
Genetic diversity of oilseed Brassica napus germplasm based on restriction fragment length polymorphisms.CrossRef | 1:STN:280:DC%2BC2c7isV2ntQ%3D%3D&md5=06295e82b31eb5db97919a87e5fff6c0CAS |

dos Santos JB, Nienhuis J, Skroch P, Tivang J, Slocum MK (1994) Comparison of RAPD and RFLP genetic markers in determining genetic similarity among Brassica oleracea L. genotypes. Theoretical and Applied Genetics 87, 909–915.
Comparison of RAPD and RFLP genetic markers in determining genetic similarity among Brassica oleracea L. genotypes.CrossRef | 1:CAS:528:DyaK2cXlslGgtL4%3D&md5=272ce92be12421a9b0fbe7e426b0edc5CAS |

Downey RK, Klassen AJ, Stringam GR (1980) Rapeseed and mustard. In ‘Hybridization of crop plants’. (Eds WR Fehr, HH Hadley) pp. 495–509. (ASA, CSSA: Madison, WI, USA)

Fu YB, Gugel RK (2010) Genetic diversity of Canadian elite summer rape (Brassica napus L.) cultivars from the pre- to post-canola quality era. Canadian Journal of Plant Science 90, 23–33.
Genetic diversity of Canadian elite summer rape (Brassica napus L.) cultivars from the pre- to post-canola quality era.CrossRef | 1:CAS:528:DC%2BC3cXivFWnsb4%3D&md5=e27af47f0c9cbdaeb3ab18e8850d2dd4CAS |

Fu D, Qian W, Zou J, Meng J (2012) Genetic dissection of intersubgenomic heterosis in Brassica napus carrying genomic components of B. rapa. Euphytica 184, 151–164.
Genetic dissection of intersubgenomic heterosis in Brassica napus carrying genomic components of B. rapa.CrossRef |

Guo Y, Chen S, Li Z, Cowling WA (2014) Center of origin and centers of diversity in an ancient crop, Brassica rapa (turnip rape). Journal of Heredity 105, 555–565.
Center of origin and centers of diversity in an ancient crop, Brassica rapa (turnip rape).CrossRef |

Gyawali S, Hegedus DD, Parkin IAP, Poon J, Higgins EE, Horner K, Bekkaoui DR, Coutu C, Buchwaldt L (2013) Genetic diversity and population structure in a world collection of accessions with emphasis on South Korea, Japan, and Pakistan. Crop Science 53, 1537–1545.
Genetic diversity and population structure in a world collection of accessions with emphasis on South Korea, Japan, and Pakistan.CrossRef |

Hasan M, Seyis F, Badani AG, Pons-Kuhnemann J, Friedt W, Lühs W, Snowdon RJ (2006) Analysis of genetic diversity in the Brassica napus L. gene pool using SSR markers. Genetic Resources and Crop Evolution 53, 793–802.
Analysis of genetic diversity in the Brassica napus L. gene pool using SSR markers.CrossRef | 1:CAS:528:DC%2BD28XmtFahsbo%3D&md5=c7aae81589e3e3049a2faf6905ef8ed1CAS |

Hobson N, Rahman H (2016) Genome-wide identification of SSR markers in the Brassica A genome and their utility in breeding. Canadian Journal of Plant Science 96, 808–818.
Genome-wide identification of SSR markers in the Brassica A genome and their utility in breeding.CrossRef | 1:CAS:528:DC%2BC28XhvVGgtrrO&md5=23c1ce70dcb6d0e9f26230798e380632CAS |

Holm SN, Rahman MH, Stølen O, Sørensen H (1985) Studies on pollination requirement in rapeseed (Brassica campestris). In ‘Advances in the production and utilization of cruciferous crops’. (Ed. H Sørensen) pp. 245–253. (Martinus Nijhoff Publishers: Dordrecht, The Netherlands)

Izzah NK, Lee J, Perumal S, Park JY, Ahn K, Fu D, Kim G-B, Nam Y-W, Yang T-J (2013) Microsatellite-based analysis of genetic diversity in 91 commercial Brassica oleracea L. cultivars belonging to six varietal groups. Genetic Resources and Crop Evolution 60, 1967–1986.
Microsatellite-based analysis of genetic diversity in 91 commercial Brassica oleracea L. cultivars belonging to six varietal groups.CrossRef | 1:CAS:528:DC%2BC3sXhs1SqsbzE&md5=58d1e817e4118aba1a163ec6d04e7d6cCAS |

Kebede B, Rahman H (2014) Quantitative trait loci (QTL) mapping of silique length and petal colour in Brassica rapa. Plant Breeding 133, 609–614.
Quantitative trait loci (QTL) mapping of silique length and petal colour in Brassica rapa.CrossRef | 1:CAS:528:DC%2BC2cXhslaltLfK&md5=417c8e2714c98a2fe7c6725951824142CAS |

Kebede B, Thiagarajah M, Zimmerli C, Rahman MH (2010) Improvement of open-pollinated spring rapeseed (Brassica napus L.) through introgression of genetic diversity from winter rapeseed. Crop Science 50, 1236–1243.
Improvement of open-pollinated spring rapeseed (Brassica napus L.) through introgression of genetic diversity from winter rapeseed.CrossRef |

Lázaro A, Aguinagalde I (1998) Genetic diversity in Brassica oleracea L. (Cruciferae) and wild relatives (2n = 18) using RAPD markers. Annals of Botany 82, 829–833.
Genetic diversity in Brassica oleracea L. (Cruciferae) and wild relatives (2n = 18) using RAPD markers.CrossRef |

Leflon M, Eber F, Letanneur JC, Chelysheva L, Coriton O, Huteau V, Ryder CD, Barker G, Jenczewski E, Chèvre AM (2006) Pairing and recombination at meiosis of Brassica rapa (AA) × Brassica napus (AACC) hybrids. Theoretical and Applied Genetics 113, 1467–1480.
Pairing and recombination at meiosis of Brassica rapa (AA) × Brassica napus (AACC) hybrids.CrossRef | 1:STN:280:DC%2BD28nlt1Kisg%3D%3D&md5=fc34a6ab2e9dc812026e265410a47de9CAS |

Li M, Chen X, Meng J (2006) Intersubgenomic heterosis in rapeseed production with a partial new-typed Brassica napus containing subgenome Ar from B. rapa and Cc from Brassica carinata. Crop Science 46, 234–242.
Intersubgenomic heterosis in rapeseed production with a partial new-typed Brassica napus containing subgenome Ar from B. rapa and Cc from Brassica carinata.CrossRef | 1:CAS:528:DC%2BD28XhsFKjsbw%3D&md5=c725fa8da13c5666b1a1eb95f9d14fbfCAS |

Li Q, Mei J, Zhang Y, Li J, Ge X, Li Z, Qian W (2013) A large-scale introgression of genomic components of Brassica rapa into B. napus by the bridge of hexaploid derived from hybridization between B. napus and B. oleracea. Theoretical and Applied Genetics 126, 2073–2080.
A large-scale introgression of genomic components of Brassica rapa into B. napus by the bridge of hexaploid derived from hybridization between B. napus and B. oleracea.CrossRef | 1:CAS:528:DC%2BC3sXht1SjsL3K&md5=ecfcc4be517d5e856ecd78e31f518571CAS |

Lu CM, Kato M, Kakihara F (2002) Destiny of a transgene escape from Brassica napus into Brassica rapa. Theoretical and Applied Genetics 105, 78–84.
Destiny of a transgene escape from Brassica napus into Brassica rapa.CrossRef | 1:CAS:528:DC%2BD38XmslGltrg%3D&md5=1b2605f951f3441dc8697a40f84fefc5CAS |

Mackay GR (1973) Interspecific hybrids between forage rape (Brassica napus L.) and turnip (Brassica campestris L. ssp. rapifera) as alternatives to forage rape. 1. An exploratory study with single pair crosses. Euphytica 22, 495–499.
Interspecific hybrids between forage rape (Brassica napus L.) and turnip (Brassica campestris L. ssp. rapifera) as alternatives to forage rape. 1. An exploratory study with single pair crosses.CrossRef |

Mason AS, Huteau V, Eber F, Coriton O, Yan G, Nelson MN, Cowling WA, Chevre AM (2010) Genome structure affects the rate of autosyndesis and allosyndesis in AABC, BBAC and CCAB Brassica interspecific hybrids. Chromosome Research 18, 655–666.
Genome structure affects the rate of autosyndesis and allosyndesis in AABC, BBAC and CCAB Brassica interspecific hybrids.CrossRef | 1:CAS:528:DC%2BC3cXhtFKrsbzI&md5=a941eaca56d8c06135e9d387ba4cf20cCAS |

McVetty PBE, Lukow OM, Hall LM, Rajcan I, Rahman H (2016) Oilseeds in North America. In ‘The world of food grains. Encyclopedia of food grains’. Vol. 1. 2nd edn (Eds C Wrigley et al.) pp. 401–408. (Elsevier: Amsterdam)

Mei J, Fu Y, Qian L, Xu X, Li J, Qian W (2011) Effectively widening the gene pool of oilseed rape (Brassica napus L.) by using Chinese B. rapa in a ‘virtual allopolyploid’ approach. Plant Breeding 130, 333–337.
Effectively widening the gene pool of oilseed rape (Brassica napus L.) by using Chinese B. rapa in a ‘virtual allopolyploid’ approach.CrossRef |

Metz PLJ, Jacobsen E, Nap JP, Pereira A, Stiekema WJ (1997) The impact on biosafety of the phosphinothricin-tolerance transgene in inter-specific B. rapa × B. napus hybrids and their successive backcrosses. Theoretical and Applied Genetics 95, 442–450.
The impact on biosafety of the phosphinothricin-tolerance transgene in inter-specific B. rapa × B. napus hybrids and their successive backcrosses.CrossRef | 1:CAS:528:DyaK2sXmtFKjurk%3D&md5=824b11c43d6c0b155d9c412e8a78663cCAS |

Mikkelsen TR, Jensen J, Jørgensen RB (1996) Inheritance of oilseed rape (Brassica napus) RAPD markers in a backcross progeny with Brassica campestris. Theoretical and Applied Genetics 92, 492–497.
Inheritance of oilseed rape (Brassica napus) RAPD markers in a backcross progeny with Brassica campestris.CrossRef | 1:STN:280:DC%2BC2c7gs1WhtA%3D%3D&md5=c477fb585c3ebe33a076cce733c71340CAS |

Nei M, Li WH (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences of the United States of America 76, 5269–5273.
Mathematical model for studying genetic variation in terms of restriction endonucleases.CrossRef | 1:CAS:528:DyaL3cXitVWn&md5=846f09325553726c98a254c2b0189391CAS |

Nicolas SD, Leflon M, Monod H, Eber F, Coriton O, Huteau V, Chèvre A-M, Jenczewski E (2009) Genetic regulation of meiotic cross-overs between related genomes in Brassica napus haploids and hybrids. The Plant Cell 21, 373–385.
Genetic regulation of meiotic cross-overs between related genomes in Brassica napus haploids and hybrids.CrossRef | 1:CAS:528:DC%2BD1MXksVKrsbk%3D&md5=84a9c64a9586fab003ffbabd703716beCAS |

Parkin I (2011) Chasing ghosts: Comparative mapping in the Brassicaceae. In ‘Genetics and genomics of the Brassicaceae. Plant genetics and genomics: crops and models 9’. (Eds R Schmidt, I Bancroft) pp. 153–170. (Springer: New York)

Qian W, Chen X, Fu D, Zou J, Meng J (2005) Intersubgenomic heterosis in seed yield potential observed in a new type of Brassica napus introgressed with partial Brassica rapa genome. Theoretical and Applied Genetics 110, 1187–1194.
Intersubgenomic heterosis in seed yield potential observed in a new type of Brassica napus introgressed with partial Brassica rapa genome.CrossRef | 1:CAS:528:DC%2BD2MXjslOrtrc%3D&md5=6753fe5ce8984253e65f34efec18c524CAS |

Qian W, Meng J, Li M, Frauen M, Sass O, Noack J, Jung C (2006) Introgression of genomic components from Chinese Brassica rapa contributes to widening the genetic diversity in rapeseed (B. napus L.), with emphasis on the evolution of Chinese rapeseed. Theoretical and Applied Genetics 113, 49–54.
Introgression of genomic components from Chinese Brassica rapa contributes to widening the genetic diversity in rapeseed (B. napus L.), with emphasis on the evolution of Chinese rapeseed.CrossRef | 1:CAS:528:DC%2BD28XlvVGrtbg%3D&md5=bb6dc1718be13535206929f658ca1aa3CAS |

Qian W, Sass O, Meng J, Li M, Frauen M, Jung C (2007) Heterotic patterns in rapeseed (Brassica napus L.): I. Crosses between spring and Chinese semi-winter lines. Theoretical and Applied Genetics 115, 27–34.
Heterotic patterns in rapeseed (Brassica napus L.): I. Crosses between spring and Chinese semi-winter lines.CrossRef | 1:STN:280:DC%2BD2szgs1Kguw%3D%3D&md5=7fd877d0d61a73db561e3f892006934cCAS |

Qian W, Li Q, Noack J, Sass O, Meng J, Frauen M, Jung C (2009) Heterotic patterns in rapeseed (Brassica napus L.): II. Crosses between European winter and Chinese semi‐winter lines. Plant Breeding 128, 466–470.
Heterotic patterns in rapeseed (Brassica napus L.): II. Crosses between European winter and Chinese semi‐winter lines.CrossRef |

Quijada P, Udall JA, Polewicz H, Vogelzang RD, Osborn TC (2004) Phenotypic effects of introgressing French winter germplasm into hybrid spring canola (Brassica napus L.). Crop Science 44, 1982–1989.

Rahman MH (2001a) Inheritance of petal colour and its independent segregation from seed colour in Brassica rapa. Plant Breeding 120, 197–200.
Inheritance of petal colour and its independent segregation from seed colour in Brassica rapa.CrossRef |

Rahman MH (2001b) Production of yellow-seeded Brassica napus through interspecific crosses. Plant Breeding 120, 463–472.
Production of yellow-seeded Brassica napus through interspecific crosses.CrossRef |

Rahman MH (2004) Optimum age of siliques for rescue of hybrid embryos from crosses between Brassica oleracea, B. rapa and B. carinata. Canadian Journal of Plant Science 84, 965–969.
Optimum age of siliques for rescue of hybrid embryos from crosses between Brassica oleracea, B. rapa and B. carinata.CrossRef |

Rahman H (2011) Use of European winter canola Brassica napus L. for the improvement of Canadian spring canola: A practical breeding example. In ‘Proceedings 13th International Rapeseed Congress’. Prague, Czech Republic. pp. 875–878. (GCIRC: Paris)

Rahman H (2013) Review: Breeding spring canola (Brassica napus L.) by the use of exotic germplasm. Canadian Journal of Plant Science 93, 363–373.
Review: Breeding spring canola (Brassica napus L.) by the use of exotic germplasm.CrossRef | 1:CAS:528:DC%2BC3sXpslCmurg%3D&md5=f30bc35ca458ef99cd7977baccb887fcCAS |

Rahman MH, Stølen O, Sørensen H, Rahman L (1996) Recurrent backcross: A method for transferring erucic acid allele into Brassica oilseed cultivars, B. campestris L. yellow sarson as an example. Acta Agriculturae Scandinavica, Section B - Soil and Plant Science 46, 68–73.

Rahman H, Harwood J, Weselake R (2013) Increasing seed oil content in Brassica species through breeding and biotechnology. Lipid Technology 25, 182–185.
Increasing seed oil content in Brassica species through breeding and biotechnology.CrossRef |

Rahman H, Kebede B, Zimmerli C, Yang R-C (2014) Genetic study and QTL mapping of seed glucosinolate content in the Brassica rapa L. Crop Science 54, 537–543.
Genetic study and QTL mapping of seed glucosinolate content in the Brassica rapa L.CrossRef | 1:CAS:528:DC%2BC2MXjtlSnurs%3D&md5=7a909f49e96a4b145fcb95ac57ccd12dCAS |

Rahman H, Bennett RA, Séguin-Swartz G (2015) Broadening genetic diversity in Brassica napus canola: Development of canola-quality spring B. napus from B. napus × B. oleracea var. alboglabra interspecific crosses. Canadian Journal of Plant Science 95, 29–41.
Broadening genetic diversity in Brassica napus canola: Development of canola-quality spring B. napus from B. napus × B. oleracea var. alboglabra interspecific crosses.CrossRef | 1:CAS:528:DC%2BC2MXhsVygt73O&md5=392a999ffd3a3e82b4a39fc04c8b2789CAS |

Rahman H, Bennett RA, Kebede B (2017) Mapping of days to flower and seed yield in spring oilseed Brassica napus carrying genome content introgressed from B. oleracea. Molecular Breeding 37, 5
Mapping of days to flower and seed yield in spring oilseed Brassica napus carrying genome content introgressed from B. oleracea.CrossRef |

Raymer PL (2002) Canola: An emerging oilseed crop. In ‘Trends in new crops and new uses’. (Eds J Janick, A Whipkey) pp. 122–126. (ASHS Press: Alexandria, VA, USA)

Rohlf FJ (2000) NTSYSpc numerical taxonomy and multivariate analysis system. Version 2.2. Exeter Software, East Setauket, NY, USA.

Rücker B, Röbbelen G (1994) Inheritance of total and individual glucosinolate contents in seeds of winter oilseed rape (Brassica napus L.). Plant Breeding 113, 206–216.
Inheritance of total and individual glucosinolate contents in seeds of winter oilseed rape (Brassica napus L.).CrossRef |

Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nature Biotechnology 18, 233–234.
An economic method for the fluorescent labeling of PCR fragments.CrossRef | 1:CAS:528:DC%2BD3cXhtVOksbk%3D&md5=a34656e73a6a8c20c60976f136db8380CAS |

Shiga T (1970) Rape breeding by interspecific crossing between Brassica napas and Brassica campestris in Japan. Japan Agricultural Research Quarterly 5, 5–10.

Simonsen V, Heneen WK (1995) Genetic variation within and among different cultivars and landraces of Brassica campestris L. and B. oleracea L. based on isozymes. Theoretical and Applied Genetics 91, 346–352.

Song KM, Osborn TC, Williams PH (1988) Brassica taxonomy based on nuclear restriction fragment length polymorphisms (RFLPs). 2. Preliminary analysis of subspecies within B. rapa (syn. campestris) and B. oleracea. Theoretical and Applied Genetics 76, 593–600.
Brassica taxonomy based on nuclear restriction fragment length polymorphisms (RFLPs). 2. Preliminary analysis of subspecies within B. rapa (syn. campestris) and B. oleracea.CrossRef | 1:CAS:528:DyaL1MXlsVGqsA%3D%3D&md5=6b55909dbd1365eda10bece5d33a65aeCAS |

Suwabe K, Tsukazaki H, Iketani H, Hatakeyama K, Kondo M, Fujimura M, Nunome T, Fukuoka H, Hirai M, Matsumoto S (2006) Simple sequence repeat-based comparative genomics between Brassica rapa and Arabidopsis thaliana: the genetic origin of clubroot resistance. Genetics 173, 309–319.
Simple sequence repeat-based comparative genomics between Brassica rapa and Arabidopsis thaliana: the genetic origin of clubroot resistance.CrossRef | 1:CAS:528:DC%2BD28XlvF2itr4%3D&md5=8ff41cb8e2030e7795174bb73e26732fCAS |

Takuno S, Kawahara T, Ohnishi O (2007) Phylogenetic relationships among cultivated types of Brassica rapa L. em. Metzg. As revealed by AFLP analysis. Genetic Resources and Crop Evolution 54, 279–285.
Phylogenetic relationships among cultivated types of Brassica rapa L. em. Metzg. As revealed by AFLP analysis.CrossRef | 1:CAS:528:DC%2BD2sXkvVKrtr8%3D&md5=be6a3a8c7755d7ea7c6b5b2332f79888CAS |

Thormann CE, Ferreira ME, Camargo LEA, Tivang JG, Osborn TC (1994) Comparison of RFLP and RAPD markers to estimating genetic relationships within and among cruciferous species. Theoretical and Applied Genetics 88, 973–980.
Comparison of RFLP and RAPD markers to estimating genetic relationships within and among cruciferous species.CrossRef | 1:STN:280:DC%2BC2c7isFSitQ%3D%3D&md5=ec0677a7f6e2547254bf2f2967d1214dCAS |

Tian E, Jiang Y, Chen L, Zou J, Liu F, Meng J (2010) Synthesis of a Brassica trigenomic allohexaploid (B. carinata × B. rapa) de novo and its stability in subsequent generations. Theoretical and Applied Genetics 121, 1431–1440.
Synthesis of a Brassica trigenomic allohexaploid (B. carinata × B. rapa) de novo and its stability in subsequent generations.CrossRef | 1:CAS:528:DC%2BC3cXhtlehtb7F&md5=df2a36fa2b9efb1a70bc49de4dfc9ac8CAS |

Udall JA, Quijada PA, Polewicz H, Vogelzang R, Osborn TC (2004) Phenotypic effects of introgressing Chinese winter and resynthesized Brassica napus L. germplasm into hybrid spring canola. Crop Science 44, 1990–1996.
Phenotypic effects of introgressing Chinese winter and resynthesized Brassica napus L. germplasm into hybrid spring canola.CrossRef |

Warwick SI, James T, Falk KC (2008) AFLP-based molecular characterization of Brassica rapa and diversity in Canadian spring turnip rape cultivars. Plant Genetic Resources 6, 11–21.
AFLP-based molecular characterization of Brassica rapa and diversity in Canadian spring turnip rape cultivars.CrossRef | 1:CAS:528:DC%2BD1cXkvVals70%3D&md5=750d58d6cca06e7929002496a28b99ecCAS |

Xiao Y, Chen L, Zou J, Tian E, Xia W, Meng J (2010) Development of a population for substantial new type Brassica napus diversified at both A/C genomes. Theoretical and Applied Genetics 121, 1141–1150.
Development of a population for substantial new type Brassica napus diversified at both A/C genomes.CrossRef |

Zaman MW (1989) Introgression in Brassica napus for adaptation to the growing conditions in Bangladesh. Theoretical and Applied Genetics 77, 721–728.
Introgression in Brassica napus for adaptation to the growing conditions in Bangladesh.CrossRef | 1:STN:280:DC%2BC2c7mvVyqsA%3D%3D&md5=0b07e4c1f2c86d181b910848194cedceCAS |

Zhang J, Li G, Li H, Pu X, Jiang J, Chai L, Zheng B, Cui C, Yang Z, Zhu Y, Jiang L (2015) Transcriptome analysis of interspecific hybrid between Brassica napus and B. rapa reveals heterosis for oil rape improvement. International Journal of Genomics 2015, 230985
Transcriptome analysis of interspecific hybrid between Brassica napus and B. rapa reveals heterosis for oil rape improvement.CrossRef |

Zhao J, Wang X, Deng B, Lou P, Wu J, Sun R, Xu Z, Vromans J, Koornneef M, Bonnema G (2005) Genetic relationships within Brassica rapa as inferred from AFLP fingerprints. Theoretical and Applied Genetics 110, 1301–1314.
Genetic relationships within Brassica rapa as inferred from AFLP fingerprints.CrossRef |

Zou J, Zhu J, Huang S, Tian E, Xiao Y, Fu D, Tu J, Fu T, Meng J (2010) Broadening the avenue of intersubgenomic heterosis in oilseed Brassica. Theoretical and Applied Genetics 120, 283–290.
Broadening the avenue of intersubgenomic heterosis in oilseed Brassica.CrossRef |



Rent Article (via Deepdyve) Export Citation