Functional Plant Biology Functional Plant Biology Society
Plant function and evolutionary biology
RESEARCH ARTICLE

Analysis of global host gene expression during the primary phase of the Arabidopsis thalianaPlasmodiophora brassicae interaction

Arati Agarwal A B F , Vijay Kaul B C , Robert Faggian D , James E. Rookes A , Jutta Ludwig-Müller E and David M. Cahill A

A School of Life and Environmental Sciences, Deakin University, Geelong Campus at Waurn Ponds, Vic. 3217, Australia.

B Department of Primary Industries, Private Bag 15, Ferntree Gully DC, Vic. 3156, Australia.

C Present address: School of Botany, The University of Melbourne, Parkville, Vic. 3010, Australia.

D Department of Primary Industries, 32 Lincoln Square Nth Carlton, PO Box 4166, Parkville, Vic. 3052, Australia.

E Department of Biology, Technische Universität Dresden, 01062 Dresden, Germany.

F Corresponding author. Email: arati.agarwal@dpi.vic.gov.au

Functional Plant Biology 38(6) 462-478 http://dx.doi.org/10.1071/FP11026
Submitted: 24 January 2011  Accepted: 31 March 2011   Published: 3 June 2011

Abstract

Microarray analysis was used to investigate changes in host gene expression during the primary stages of the interaction between the susceptible plant Arabidopsis thaliana (L.) Heynh ecotype Col-0 and the biotrophic pathogen Plasmodiophora brassicae Woronin. Analyses were conducted at 4, 7 and 10 days after inoculation (DAI) and revealed significant induction or suppression of a relatively low number of genes in a range of functional categories. At 4 DAI, there was induced expression of several genes known to be critical for pathogen recognition and signal transduction in other resistant host–pathogen interactions. As the pathogen further colonised root tissue and progressed through the primary plasmodium stage to production of zoosporangia at 7 and 10 DAI, respectively, fewer genes showed changes in expression. The microarray results were validated by examining a subset of induced genes at 4 DAI by quantitative real-time reverse transcriptase PCR (RT-qPCR) analysis all of which correlated positively with the microarray data. The two A. thaliana mutants jar1 and coiI tested were found to be susceptible to P. brassicae. The involvement of defence-related hormones in the interaction was further investigated and the findings indicate that addition of salicylic acid can suppress clubroot disease in A. thaliana plants.

Additional keywords: ATH1 microarray chip, clubroot, compatibility, real-time RT-qPCR, salicylic acid.


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