Functional Plant Biology Functional Plant Biology Society
Plant function and evolutionary biology
RESEARCH FRONT

Salinity drives host reaction in Phaseolus vulgaris (common bean) to Macrophomina phaseolina

Ming Pei You A C , Timothy D. Colmer A B and Martin J. Barbetti A B

A School of Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.

B The UWA Institute of Agriculture, Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.

C Corresponding author. Email: mingpei.you@uwa.edu.au

Functional Plant Biology 38(12) 984-992 http://dx.doi.org/10.1071/FP11137
Submitted: 9 June 2011  Accepted: 20 October 2011   Published: 17 November 2011

Abstract

Productivity of Phaseolus vulgaris L. (common bean) is often limited by diseases such as seedling blight and root and stem rot caused by the fungus Macrophomina phaseolina and by abiotic stresses such as salinity. This paper reports controlled environment studies examining the interaction of biotic (M. phaseolina) and abiotic (NaCl) stresses. Studies were conducted at 32°C. On potato dextrose agar, the growth of two isolates of M. phaseolina (M1, M2) was differentially stimulated by 40 mM NaCl with 1 mM CaSO4. M. phaseolina was applied as either soil-borne inoculum or directly injected into P. vulgaris hypocotyls. For direct hypocotyl inoculation experiments, there was no difference in disease severity resulting from the two isolates. However, when soil inoculation was undertaken, isolate M2 caused more disease than M1. Addition of 40 mM NaCl to the soil increased disease development and severity (evident 4 days after inoculation), particularly as demonstrated in the hypocotyl inoculation tests, suggesting that salinity stress predisposes plants to infection by this pathogen. Plants infested by M. phaseolina showed increased tissue concentrations of Na+ and Cl but decreased K+ concentration. Hypocotyls generally contained higher Na+ concentrations than shoots. Inoculated plants had higher Na+ and lower K+ concentrations than uninoculated plants. Our studies indicate that M. phaseolina will be a more severe disease threat where P. vulgaris is cultivated in areas affected by soil salinity.

Additional keywords: ashy grey stem, biotic-abiotic interactions charcoal rot, Macrophomina phaseolina.


References

Acosta-Gallegos J (1998) Mejoramiento genetico del frijol en Mexico. In ‘Memoria del Taller Internacional de Majoramiento Genetico de Frijol Negro Mesoamericano’. (Ed. Lepiz-Ildefenso) pp. 5–12. (Profrijol: Veracruz, Mexico)

Amtmann A, Troufflard S, Armengaud P (2008) The effect of potassium nutrition on pest and disease resistance in plants. Physiologia Plantarum 133, 682–691.
The effect of potassium nutrition on pest and disease resistance in plants.CrossRef | 1:CAS:528:DC%2BD1cXps1Oit7s%3D&md5=bb71f6245909221787fb12385d0edd5bCAS | open url image1

Bayuelo-Jiménez J, Craig R, Lynch J (2002a) Salinity tolerance of Phaseolus species during germination and early seedling growth. Crop Science 42, 1584–1594.
Salinity tolerance of Phaseolus species during germination and early seedling growth.CrossRef | open url image1

Bayuelo-Jiménez J, Debouck D, Lynch J (2002b) Salinity tolerance of Phaseolus species during early vegetative growth. Crop Science 42, 2184–2192.
Salinity tolerance of Phaseolus species during early vegetative growth.CrossRef | open url image1

Benito B, Garciadeblás B, Schreier P, Rodríguez-Navarro A (2004) Novel P-Type ATPases mediate high-affinity potassium or sodium uptake in fungi. Eukaryotic Cell 3, 359–368.
Novel P-Type ATPases mediate high-affinity potassium or sodium uptake in fungi.CrossRef | 1:CAS:528:DC%2BD2cXjtlKqsr4%3D&md5=4aa0722fc36ae7392a9953a390e561a8CAS | open url image1

Blumwald E (2000) Sodium transport and salt tolerance in plants. Current Opinion in Cell Biology 12, 431–434.
Sodium transport and salt tolerance in plants.CrossRef | 1:CAS:528:DC%2BD3cXlt1Srtbg%3D&md5=d1c0b6ba7b4401cb324c339a56e3e30fCAS | open url image1

Bouchibi N, Van Brugcen AHC, MacDonald JC (1990) Effect of ion concentration and sodium: calcium ratio of a nutrient solution on phytophthora root of tomato and zoospore motility and viability of Phvtophthora parasitica. Phytopathology 80, 1323–1336.
Effect of ion concentration and sodium: calcium ratio of a nutrient solution on phytophthora root of tomato and zoospore motility and viability of Phvtophthora parasitica.CrossRef | 1:CAS:528:DyaK3MXhtFyksrY%3D&md5=7d3160aca6bb1df622bfa0b77a0471fdCAS | open url image1

Boursier P, Lynch J, Lauchli A, Epstein E (1987) Chloride partitioning in leaves of salt stressed sorghum, maize, wheat and barley. Australian Journal of Plant Physiology 14, 463–473.
Chloride partitioning in leaves of salt stressed sorghum, maize, wheat and barley.CrossRef | 1:CAS:528:DyaL1cXlt1Sq&md5=f277f1e6a2b0eb63563849fef96bfef8CAS | open url image1

Collinge DB, Jørgensen HJL, Lund OS, Lyngkjær MF (2010) Engineering pathogen resistance in crop plants: current trends and future prospects. Annual Review of Phytopathology 48, 269–291.
Engineering pathogen resistance in crop plants: current trends and future prospects.CrossRef | 1:CAS:528:DC%2BC3cXht1Wgt7%2FI&md5=2023c6a2b26126800188a3816256a88cCAS | open url image1

Dhingra O, Sinclair J (1987) ‘Biology and pathology of Macrophomina phaseolina.’ (Universidad Federal de Viçosa: Viçosa, Brazil)

Echavez-Badel R, Beaver JS (1987) Dry bean genotypes and Macrophomina phaseolina (Tassi) Goid in inoculated and noninoculated field plots. Journal of Agriculture of the University of Puerto Rico 71, 385–390.

El-Abyad MS, Hindorf H, Rizk MA (1988) Impact of salinity stress on soil-borne fungi of sugarbeet. I. Pathogenicity implications. Plant and Soil 110, 27–32.
Impact of salinity stress on soil-borne fungi of sugarbeet. I. Pathogenicity implications.CrossRef | open url image1

El Mahjoub M, Bouzaidi A, Jouhri A, Hamrouni A, Beji EL (1979) Influence de la salinité des eaux d’irrigation sur la sensibilité du Tournesol au Macrophomina phaseoli (Maubl.) Ashby. Annales de Phytopathologie 11, 61–67.

Fang X, Phillips D, Li H, Sivasithamparam K, Barbetti MJ (2011) Comparisons of virulence of pathogens associated with crown and root diseases of strawberry in Western Australia with special reference to the effect of temperature. Scientia Horticulturae 131, 39–48.
Comparisons of virulence of pathogens associated with crown and root diseases of strawberry in Western Australia with special reference to the effect of temperature.CrossRef | open url image1

FAO (2005) FAO Stat statistical database. Food and Agriculture Organization of the United Nations. Available at http://faostat.fao.org/default.htm [Verified 27 October 2011]

Gama P, Inanaga S, Tanaka K, Nakazawa R (2007) Physiological response of common bean (Phaseolus vulgaris L.) seedlings to salinity stress. African Journal of Biotechnology 6, 79–88.

Gray F, Mihail J, Lavigne R, Porter P (1991) Incidence of charcoal rot of sorghum and soil populations of Macrophomina phaseolina associated with sorghum and native vegetation in Somalia. Mycopathologia 114, 145–151.
Incidence of charcoal rot of sorghum and soil populations of Macrophomina phaseolina associated with sorghum and native vegetation in Somalia.CrossRef | open url image1

Hasegawa P, Bressan R, Zhu J-K, Bohnert H (2000) Plant cellular and molecular responses to high salinity. Annual Review of Plant Physiology and Plant Molecular Biology 51, 463–499.
Plant cellular and molecular responses to high salinity.CrossRef | 1:CAS:528:DC%2BD3cXlsVymt7s%3D&md5=6cb239a13a0581221e65204097647e08CAS | open url image1

Läuchli A (1984) Salt exclusion: an adaptation of legumes for crops and pastures under saline conditions. In ‘Salinity tolerance in plants. Strategies for crop improvement’. pp. 171–187. (John Wiley & Sons: New York)

Manici LM, Caputo F, Cerato C (1995) Temperature responses of isolates of Macrophomina phaseolina from different climatic regions of sunflower production in Italy. Plant Disease 79, 834–838.

Mayek-Perez N, Lopez-Castaneda C, Gonzalez-Chavira M, Garcia-Espinosa R, Acosta-Gallegos J, Martinez De La Bega O, Simpson J (2001) Variability of Mexican isolates of Macrophomina phaseolina based on the pathogenesis and AFLP genotype. Physiological and Molecular Plant Pathology 59, 257–264.
Variability of Mexican isolates of Macrophomina phaseolina based on the pathogenesis and AFLP genotype.CrossRef | 1:CAS:528:DC%2BD3MXovV2mtrY%3D&md5=1a89542b7ae9e00b153c1ff0ef124d90CAS | open url image1

Mayek-Perrez N, Lopez-Castaneda C, Acosta-Gallegos J (1997) Variacion en caracteristicas culturales in vitro de aislamientos de Macrophomina phaseolina y su virulencia en frijol. Agrociencia 31, 187–195.

Mayek-Perrez N, Lopez-Castaneda C, Lopez-Salinas E, Cumpian-Gutierrez J, Joaquin-Torres I, Padilla-Ramirez J, Acosta-Gallegos J (2003) Effect of Macrophomina phaseolina (Tassi) Goid. on grain yield of common beans (Phaseolus vulgaris L.) and its relationship with yield stability parameters. Revista Mexicana de Fitopatologia 21, 168–175.

Mihail J, Taylor S (1995) Interpreting variability among isolates of Macrophomina phaseolina in pathogenicity, pycnidium production, and chlorate utilization. Canadian Journal of Botany 73, 1596–1603.
Interpreting variability among isolates of Macrophomina phaseolina in pathogenicity, pycnidium production, and chlorate utilization.CrossRef | open url image1

Munns R, Tester M (2008) Mechanisms of salinity tolerance. Annual Review of Plant Biology 59, 651–681.
Mechanisms of salinity tolerance.CrossRef | 1:CAS:528:DC%2BD1cXntFaqtrw%3D&md5=4ff74227fb3898bf12208db44bd191acCAS | open url image1

Nischwitz C, Olsen M, Rasmussen S (2002) Influence of salinity and root-knot nematode as stress factors in charcoal rot of melon. 2002 Vegetable Report. University of Arizona College of Agriculture and Life Sciences. Available at http://ag.arizona.edu/pubs/crops/az1292/ [Verified 27 October 2011]

Pardo JM, Quintero FJ (2002) Plants and sodium ions: keeping company with the enemy. Genome Biology 3, 1014–1017.
Plants and sodium ions: keeping company with the enemy.CrossRef | open url image1

Redden R, Tompkins W, Usher T (1997) Growth interactions of navy bean varieties with sowing date and season. Australian Journal of Experimental Agriculture 37, 213–221.
Growth interactions of navy bean varieties with sowing date and season.CrossRef | open url image1

Rodriguez-Navarro A (2000) Potassium transport in fungi and plants. Biochimica et Biophysica Acta 1469, 1–30.

Sanchez A (1989) ‘Catastro sobre incidencia de Macrophomina phaseolina en la zona de San Juan, R.D. Informe final.’ (Regional Suroeste: SEA, Reptiblica Dominicana)

Schwartz H, Pastor Corrales M (1989) ‘Bean production problems in the tropics.’ 2nd edn. (CIAT: Cali, Colombia)

Sinclair J (1982) ‘Compendium of soybean diseases.’ 2nd edn. (American Phytopathological Society: St Paul, MN, USA)

Snapp SS, Shennan C, Van Bruggen AHC (1991) Effects of salinity on severity of infection by Phytophthora parasitica Dast, ion concentrations and growth of tomato, Lycopersicon esculentum Mill. New Phvtologist 119, 275–284.
Effects of salinity on severity of infection by Phytophthora parasitica Dast, ion concentrations and growth of tomato, Lycopersicon esculentum Mill.CrossRef | 1:CAS:528:DyaK38XhvVGgug%3D%3D&md5=414cb33376d615c96f7eac9c335f5b57CAS | open url image1

Srivastava A, Singh T, Jana T, Arora D (2001) Microbial colonization of Macrophomina phaseolina and suppression of charcoal rot of chickpea. In ‘Microbes and plants’. (Ed. A Sinha) pp. 269–319. (Vedams eBooks Pty Ltd: New Delhi)

Swiecki TJ (1984) Interactionsbetween salinity and Phytophthora root rots of chrysanthemum and tomato. PhD Thesis, Plant Pathology Department, University of California, Davis, CA.

Tavakkoli E, Fatehi F, Coventry S, Rengasamy P, McDonald GK (2011) Additive effects of Na+ and Cl ions on barley growth under salinity stress. Journal of Experimental Botany 62, 2189–2203.
Additive effects of Na+ and Cl ions on barley growth under salinity stress.CrossRef | 1:CAS:528:DC%2BC3MXjsFyju74%3D&md5=f38691f864065ee74a6719661f466f15CAS | open url image1

Tester M, Davenport R (2003) Na+ tolerance and Na transportation in higher plants. Annals of Botany 91, 503–527.
Na+ tolerance and Na transportation in higher plants.CrossRef | 1:CAS:528:DC%2BD3sXjsVyisbk%3D&md5=1d347e97621c3429b50d3bd9471ffd5eCAS | open url image1

Tyagi H, Rajasubramaniam S, Rajam M, Dasgupta I (2008) RNA-interference in rice against rice tungro bacilliform virus results in its decreased accumulation in inoculated rice plants. Transgenic Research 17, 897–904.
RNA-interference in rice against rice tungro bacilliform virus results in its decreased accumulation in inoculated rice plants.CrossRef | 1:CAS:528:DC%2BD1cXhtValt7vL&md5=d1fe871cd4e68d827ce50c397bce6109CAS | open url image1

Watson A (2009) Soil-borne diseases of beans. In ‘Primefact 586’. (Department of Industry & Investment: Ourimbah, NSW)

Wrather J, Anderson T, Arsyad D, Gai J, Ploper L, Porta-Puglia A, Ram H, Yorinori J (1997) Soybean disease loss estimates for the top 10 soybean producing countries in 1994. Plant Disease 81, 107–110.
Soybean disease loss estimates for the top 10 soybean producing countries in 1994.CrossRef | open url image1

Wrather J, Anderson T, Arsyad D, Tan Y, Ploper L, Porta-Puglia A, Ram H, Yorinori J (2001) Soybean disease loss estimates for the top 10 soybean producing countries in 1998. Canadian Journal of Plant Pathology 23, 115–121.
Soybean disease loss estimates for the top 10 soybean producing countries in 1998.CrossRef | open url image1

Zhu J (2003) Regulation of ion homeostasis under salt stress. Current Opinion in Plant Biology 6, 441–445.
Regulation of ion homeostasis under salt stress.CrossRef | 1:CAS:528:DC%2BD3sXntVKhsbs%3D&md5=7e54c59bd255571504f5829ff758b75fCAS | open url image1


Full Text PDF (165 KB) Export Citation