Functional Plant Biology Functional Plant Biology Society
Plant function and evolutionary biology
RESEARCH ARTICLE (Open Access)

Post-anthesis heat and a Gpc-B1 introgression have similar but non-additive effects in bread wheat

Lancelot Maphosa A B C , Nicholas C. Collins A B , Julian Taylor B and Diane E. Mather A B D
+ Author Affiliations
- Author Affiliations

A Australian Centre for Plant Functional Genomics, Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA 5064, Australia.

B School of Agriculture, Food and Wine, Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA 5064, Australia.

C Present address: Department of Environment and Primary Industries, 110 Natimuk Road, Horsham, Vic. 3400, Australia.

D Corresponding author. Email: diane.mather@adelaide.edu.au

Functional Plant Biology 41(9) 1002-1008 https://doi.org/10.1071/FP14060
Submitted: 22 February 2014  Accepted: 8 May 2014   Published: 10 June 2014

Abstract

High temperatures during grain filling can reduce the yield of wheat and affect its grain protein concentration. The Gpc-B1 locus of wheat also affects grain protein concentration, but it is not known whether its effects interact with those of heat. The aim of this study was to investigate the effects of high temperature in lines with and without functional (high-protein) alleles at Gpc-B1. A highly replicated experiment was conducted in a glasshouse under control conditions (24/18°C, 14/10 h day/night), with half of the plants of each line or cultivar put into a heat chamber (37/27°C, 14/10 h day/night) at 15 days after anthesis for 3 days. Backcross derivatives with the Gpc-B1 introgression segment differed from their recurrent parents more than those without that segment. In some respects, the effects of the Gpc-B1 introgression were similar to those of the heat treatment: both could accelerate peduncle senescence, increase grain protein content and increase the percentage of unextractable polymeric protein. Unlike the heat treatment, Gpc-B1 did not reduce grain weight, indicating that factors that hasten senescence do not necessarily limit grain size. The presence of the Gpc-B1 segment did not exacerbate the effects of heat stress on any trait.

Additional keywords: protein, senescence, single nucleotide polymorphism, stress.


References

Altenbach SB (2012) New insights into the effects of high temperature, drought and post-anthesis fertilizer on wheat grain development. Journal of Cereal Science 56, 39–50.
New insights into the effects of high temperature, drought and post-anthesis fertilizer on wheat grain development.CrossRef |

Appelbee MJ (2007) Quality potential of gluten proteins in hexaploid wheat and related species. PhD Thesis, School of Agriculture, Food and Wine, The University of Adelaide, SA, Australia.

Balla K, Rakszegi M, Li Z, Békés F, Bencze S, Veisz O (2011) Quality of winter wheat in relation to heat and drought shock after anthesis. Czech Journal of Food Sciences 29, 117–128.

Batey IL, Gupta RB, MacRitchie F (1991) Use of size-exclusion high-performance liquid chromatography in the study of wheat flour proteins: an improved chromatographic procedure. Cereal Chemistry 68, 207–209.

Beecher FW, Mason E, Mondal S, Awika J, Dirk Hays D, Ibrahim A (2012) Identification of quantitative trait loci (QTLs) associated with maintenance of wheat (Triticum aestivum Desf.) quality characteristics under heat stress conditions. Euphytica 188, 361–368.
Identification of quantitative trait loci (QTLs) associated with maintenance of wheat (Triticum aestivum Desf.) quality characteristics under heat stress conditions.CrossRef | 1:CAS:528:DC%2BC38Xhs12rsLrK&md5=006bb398d6ceabbcce44739ba5b5b3cdCAS |

Blumenthal CS, Bekes F, Gras PW, Barlow EWR, Wrigley CW (1995) Identification of wheat genotypes tolerant to the effects of heat stress on grain quality. Cereal Chemistry 72, 539–544.

Brevis JC, Dubcovsky J (2010) Effects of the chromosome region including the Gpc-B1 locus on wheat grain and protein yield. Crop Science 50, 93–104.
Effects of the chromosome region including the Gpc-B1 locus on wheat grain and protein yield.CrossRef | 1:CAS:528:DC%2BC3cXhvFSkt74%3D&md5=446a492acaf0a5c484b0c61063ae2a45CAS |

Brevis JC, Morris CF, Manthey F, Dubcovsky J (2010) Effect of the grain protein content locus Gpc-B1 on bread and pasta quality. Journal of Cereal Science 51, 357–365.
Effect of the grain protein content locus Gpc-B1 on bread and pasta quality.CrossRef | 1:CAS:528:DC%2BC3cXmsVeksrw%3D&md5=950643f2bde3355d65155dc83b89f829CAS |

Buckee GK (1994) Determination of total nitrogen in barley, malt and beer by Kjeldahl procedures and the Dumas combustion method-collaborative trial. Journal of the Institute of Brewing. Institute of Brewing 100, 57–64.

Butler DG, Cullis BR, Gilmour AR, Gogel BJ (2009) ‘ASReml-R Reference Manual.’ (ver. 3) (Queensland Department of Primary Industries: Brisbane)

Carceller JL, Aussenac T (1999) Accumulation and changes in molecular size distribution of polymeric proteins in developing grains of hexaploid wheats: role of the desiccation phase. Australian Journal of Plant Physiology 26, 301–310.
Accumulation and changes in molecular size distribution of polymeric proteins in developing grains of hexaploid wheats: role of the desiccation phase.CrossRef | 1:CAS:528:DyaK1MXlvFGlsLc%3D&md5=0661a77b2f9b0a2ca8bb42d524a954c9CAS |

Cavanagh C, Taylor J, Larroque O, Coombes N, Verbyla A, Nath Z, Kutty I, Rampling L, Butow B, Ral J-P, Tomoskozi S, Balazs G, Békés F, Mann G, Quail K, Southan M, Morell M, Newberry M (2010) Sponge and dough bread making: genetic and phenotypic relationships with wheat quality traits. Theoretical and Applied Genetics 121, 815–828.
Sponge and dough bread making: genetic and phenotypic relationships with wheat quality traits.CrossRef | 20495901PubMed |

Cavanagh CR, Chao S, Wang S, Huang BE, Stephen S, Kiani S, Forrest K, Saintenac C, Brown-Guedira GL, Akhunova A, See D, Bai G, Pumphrey M, Tomar L, Wong D, Kong S, Reynolds M, da Silva ML, Bockelman H, Talbert L, Anderson JA, Dreisigacker S, Baenziger S, Carter A, Korzun V, Morrell PL, Dubcovsky J, Morell MK, Sorrells ME, Hayden MJ, Akhunov E (2013) Genome-wide comparative diversity uncovers multiple targets of selection for improvement in hexaploid wheat landraces and cultivars. Proceedings of the National Academy of Sciences of the United States of America 110, 8057–8062.
Genome-wide comparative diversity uncovers multiple targets of selection for improvement in hexaploid wheat landraces and cultivars.CrossRef | 1:CAS:528:DC%2BC3sXhtV2ksL%2FJ&md5=2ac518560f5795dc4c80dd01c1e5c5bfCAS | 23630259PubMed |

Distelfeld A, Cakmak I, Peleg Z, Ozturk L, Yazici AM, Budak H, Saranga Y, Fahima T (2007) Multiple QTL-effects of wheat Gpc-B1 locus on grain protein and micronutrient concentrations. Physiologia Plantarum 129, 635–643.
Multiple QTL-effects of wheat Gpc-B1 locus on grain protein and micronutrient concentrations.CrossRef | 1:CAS:528:DC%2BD2sXisVyku7o%3D&md5=d5df32d7b39b669035200d9fc33fb000CAS |

Eagles HA, Hollamby GJ, Gororo NN, Eastwood RF (2002) Estimation and utilisation of glutenin gene effects from the analysis of unbalanced data from wheat breeding programs. Australian Journal of Agricultural Research 53, 367–377.
Estimation and utilisation of glutenin gene effects from the analysis of unbalanced data from wheat breeding programs.CrossRef | 1:CAS:528:DC%2BD38XjtlWju7c%3D&md5=e0a91fa7028c45fdd53c8624ae44e8b8CAS |

Fleury D, Jefferies S, Kuchel H, Langridge P (2010) Genetic and genomic tools to improve drought tolerance in wheat. Journal of Experimental Botany 61, 3211–3222.
Genetic and genomic tools to improve drought tolerance in wheat.CrossRef | 1:CAS:528:DC%2BC3cXptVymsLc%3D&md5=5c66e9f2b8ff0fa2bfa7a74aedbabd61CAS | 20525798PubMed |

Gilmour AR, Cullis BR, Verbyla AP (1997) Accounting for natural and extraneous variation in the analysis of field experiments. Journal of Agricultural Biological & Environmental Statistics 2, 269–293.
Accounting for natural and extraneous variation in the analysis of field experiments.CrossRef |

Gupta RB, Masci S, Lafiandra D, Bariana HS, MacRitchie F (1996) Accumulation of protein subunits and their polymers in developing grains of hexaploid wheats. Journal of Experimental Botany 47, 1377–1385.
Accumulation of protein subunits and their polymers in developing grains of hexaploid wheats.CrossRef | 1:CAS:528:DyaK28XmslWlsro%3D&md5=1410259f110a3f7a8a4d3355e882a358CAS |

Irmak S, Naeem HA, Lookhart GL, MacRitchie F (2008) Effect of heat stress on wheat proteins during kernel development in wheat near-isogenic lines differing at Glu-D1. Journal of Cereal Science 48, 513–516.
Effect of heat stress on wheat proteins during kernel development in wheat near-isogenic lines differing at Glu-D1.CrossRef | 1:CAS:528:DC%2BD1cXhtVarsLnM&md5=1c68e81ac0a227501d612235549ec5c9CAS |

Jenner CF (1994) Starch synthesis in the kernel of wheat under high temperature conditions. Australian Journal of Plant Physiology 21, 791–806.
Starch synthesis in the kernel of wheat under high temperature conditions.CrossRef | 1:CAS:528:DyaK2MXkslequrk%3D&md5=f5f6080f04f261a55928fd045d2b3c93CAS |

Li Y, Wu Y, Hernandez-Espinosa N, Peña RJ (2013) The influence of drought and heat stress on the expression of end use quality parameters of common wheat. Journal of Cereal Science 57, 73–78.
The influence of drought and heat stress on the expression of end use quality parameters of common wheat.CrossRef | 1:CAS:528:DC%2BC38Xhs1ajsb%2FE&md5=331e29198c7f8a584b0d0730dcbca3d6CAS |

Olmos S, Distelfeld A, Chicaiza O, Schlatter AR, Fahima T, Echenique V, Dubcovsky J (2003) Precise mapping of a locus affecting grain protein content in durum wheat. Theoretical and Applied Genetics 107, 1243–1251.
Precise mapping of a locus affecting grain protein content in durum wheat.CrossRef | 1:CAS:528:DC%2BD3sXos1Oktbw%3D&md5=66be8fd528d90737b4cad188d16818e9CAS | 12923624PubMed |

Pallotta MA, Graham RD, Langridge P, Sparrow DHB, Barker SJ (2000) RFLP mapping of manganese efficiency in barley. Theoretical and Applied Genetics 101, 1100–1108.
RFLP mapping of manganese efficiency in barley.CrossRef | 1:CAS:528:DC%2BD3cXovVaks7o%3D&md5=6c18b7839fb50bce6844e8ecc6a36196CAS |

R Development Core Team (2012) ‘R: a language and environment for statistical computing.’ (R Foundation for Statistical Computing: Vienna)

Randall P, Moss H (1990) Some effects of temperature regime during grain filling on wheat quality. Australian Journal of Agricultural Research 41, 603–617.
Some effects of temperature regime during grain filling on wheat quality.CrossRef |

Stone P, Nicolas M (1995a) Effect of timing of heat stress during grain filling on two wheat varieties differing in heat tolerance. I. Grain growth. Functional Plant Biology 22, 927–934.

Stone P, Nicolas M (1995b) Comparison of sudden heat stress with gradual exposure to high temperature during grain filling in two wheat varieties differing in heat tolerance. I. Grain growth. Functional Plant Biology 22, 935–944.

Stone PJ, Nicolas ME (1996) Varietal differences in mature protein composition of wheat resulted from different rates of polymer accumulation during grain filling. Australian Journal of Plant Physiology 23, 727–737.
Varietal differences in mature protein composition of wheat resulted from different rates of polymer accumulation during grain filling.CrossRef | 1:CAS:528:DyaK2sXms1Ggsg%3D%3D&md5=b032d3e461edd36e7464c66cc1a0b540CAS |

Tashiro T, Wardlaw IF (1989) A comparison of the effect of high temperature on grain development in wheat and rice. Annals of Botany 64, 59–65.

Uauy C, Brevis JC, Dubcovsky J (2006a) The high grain protein content gene Gpc-B1 accelerates senescence and has pleiotropic effects on protein content in wheat. Journal of Experimental Botany 57, 2785–2794.
The high grain protein content gene Gpc-B1 accelerates senescence and has pleiotropic effects on protein content in wheat.CrossRef | 1:CAS:528:DC%2BD28Xotl2mtbk%3D&md5=5a845fc25dd0f078b2db60fdfaae61c9CAS | 16831844PubMed |

Uauy C, Distelfeld A, Fahima T, Blechl A, Dubcovsky J (2006b) A NAC gene regulating senescence improves grain protein, zinc, and iron content in wheat. Science 314, 1298–1301.
A NAC gene regulating senescence improves grain protein, zinc, and iron content in wheat.CrossRef | 1:CAS:528:DC%2BD28Xht1agt7jL&md5=ccd00356986d0bb068bd97b3243308b6CAS | 17124321PubMed |

Viswanathan C, Khanna-Chopra R (2001) Effect of heat stress on grain growth, starch synthesis and protein synthesis in grains of wheat (Triticum aestivum L.) varieties differing in grain weight stability. Journal Agronomy & Crop Science 186, 1–7.
Effect of heat stress on grain growth, starch synthesis and protein synthesis in grains of wheat (Triticum aestivum L.) varieties differing in grain weight stability.CrossRef | 1:CAS:528:DC%2BD3MXjt1Smsb8%3D&md5=951f9674daf57f2b9d76124d2524672fCAS |

Waters BM, Uauy C, Dubcovsky J, Grusak MA (2009) Wheat (Triticum aestivum) NAM proteins regulate the translocation of iron, zinc, and nitrogen compounds from vegetative tissues to grain. Journal of Experimental Botany 60, 4263–4274.
Wheat (Triticum aestivum) NAM proteins regulate the translocation of iron, zinc, and nitrogen compounds from vegetative tissues to grain.CrossRef | 1:CAS:528:DC%2BD1MXhtlGitL%2FN&md5=5444ebe0beed8b59de5d4e83aad371e4CAS | 19858116PubMed |

Zahedi M, Jenner CF (2003) Analysis of effects in wheat of high temperature on grain filling attributes estimated from mathematical models of grain filling. Journal of Agricultural Science 141, 203–212.
Analysis of effects in wheat of high temperature on grain filling attributes estimated from mathematical models of grain filling.CrossRef |


Full Text PDF (238 KB) Export Citation Cited By (6)