
TY  - JOUR
A1  - Smouni, Aziz 
A1  - Laplaze, Laurent 
A1  - Bogusz, Didier 
A1  - Guermache, Fathia 
A1  - Auguy, Florence 
A1  - Duhoux, Emile 
A1  - Franche, Claudine 
T1  - <emph type="2">Research note</emph>: The 35S promoter is not constitutively expressed in the transgenic tropical actinorhizal tree <emph type="2">Casuarina glauca</emph>
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 2002
VL  - 29
IS  - 5
SP  - 649
EP  - 656
KW  - Agrobacterium-mediated genetic transformation, CaMV 35S promoter, Casuarina glauca, transgenic actinorhizal nodules, &#946;-glucuronidase gene.
UR  - https://doi.org/10.1071/PP01121 
N2  - The tropical nitrogen-fixing tree, Casuarina glauca Sieb. ex Spreng. was genetically transformed using Agrobacterium tumefaciens C58C1(pGV2260; pBIN19GUSINT). We report on the expression pattern conferred by the cauliflower mosaic virus (CaMV) 35S promoter in transgenic C. glauca plants grown in vitro, and for one year in a greenhouse. Histochemical assays in shoots from in vitro plants revealed &#946;-glucuronidase (GUS) staining in apical and axillary buds, and in nearly all tissues near the base of the stem. In roots, the CaMV 35S drove strong GUS expression in the apex and vascular tissue. In 1-year old plants grown in a greenhouse, the CaMV 35S promoter was highly active, except in peripheral suberized tissues. Transgenic C. glauca plants were nodulated by the actinomycete Frankia. Histochemical assays on vibratome sections of transgenic nodules demonstrated intense GUS activity in the vascular bundle, the phellogen, and in strands of uninfected cells filled with polyphenols. GUS expression was undetectable in Frankia-infected cells. 
ER  -

