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Protocols in ecological and environmental plant physiology


Article << Previous     |     Next >>   Contents Vol 52(12)

Microsatellites as markers for Australian wheat improvement

N. Harker, L. R. Rampling, M. R. Shariflou, M. J. Hayden, T. A. Holton, M. K. Morell, P. J. Sharp, R. J. Henry and K. J. Edwards

Australian Journal of Agricultural Research 52(12) 1121 - 1130
Published: 15 November 2001


Microsatellite markers have been shown to be highly polymorphic and simple to use in hexaploid wheat. This study aimed to establish microsatellites as informative markers for Australian wheat improvement. By screening microsatellites developed as part of the Wheat Microsatellite Consortium and other available microsatellite sources, 257 informative microsatellites for Australian wheat varieties were identified and reported in the Australian National Wheat Molecular Marker Program microsatellite database (http://www.scu.edu.au/research/cpcg/). Of these, 151 microsatellites identifying 172 loci were scored on at least 1 of 4 double haploid mapping populations and were then integrated, where possible, into existing genetic maps. Polymorphism information content values were calculated for most microsatellites to establish a reference for their value for future investigations. The mapping of available microsatellites enhances the quality of the genetic maps and may provide useful genetic markers for traits of interest to the Australian wheat breeding programs.

Keywords: database, genetic map, PIC values.

Full text doi:10.1071/AR01025

© CSIRO 2001

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