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Protocols in ecological and environmental plant physiology

 

Article << Previous     |         Contents Vol 29(8)

Research note: Rapid isolation of total RNA and genomic DNA from Hakea actities

Michael G. Mason and Susanne Schmidt

Functional Plant Biology 29(8) 1015 - 1016
Published: 05 August 2002

Abstract

Tissues of the Australian native plant species Hakea actities (Proteaceae) contain numerous metabolites and structural compounds that hinder the isolation of nucleic acids. Separate RNA and genomic DNA extraction procedures were developed to isolate high quality nucleic acids from H. actities. Total RNA was extracted from leaves, roots and cluster roots of H. actities grown in low nutrient levels. Cluster root formation in H. actities only occurs when the plants are grown in low nutrient concentrations. However, under these conditions, nucleic acid extraction becomes increasingly difficult. The new procedures are faster than many of the published nucleic acid extraction protocols, and avoid the use of hazardous chemicals. The RNA extraction method was used successfully on another Australian species and a crop species, suggesting that the procedure is useful for molecular studies of a broad range of plants.

Keywords: Australian native plants, cluster roots, genomic DNA extraction, Hakea actities, RNA extraction.



Full text doi:10.1071/PP01151

© CSIRO 2002

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