Invertebrate Systematics Invertebrate Systematics Society
Systematics, phylogeny and biogeography
RESEARCH ARTICLE

Barcoding type specimens helps to identify synonyms and an unnamed new species in Eumunida Smith, 1883 (Decapoda : Eumunididae)

Nicolas Puillandre A E , Enrique Macpherson B , Josie Lambourdière C , Corinne Cruaud D , Marie-Catherine Boisselier-Dubayle A C and Sarah Samadi A C
+ Author Affiliations
- Author Affiliations

A ‘Systématique, Adaptation et Evolution’, UMR 7138 UPMC-IRD-MNHN-CNRS (UR IRD 148), Muséum national d’Histoire naturelle, Département Systématique et Evolution, CP 26, 57 Rue Cuvier, F-75231 Paris Cedex 05, France.

B Centro de Estudios Avanzados de Blanes (CEAB-CSIC), C. acc. Cala Sant Francesc 14, 17300 Blanes, Girona, Spain.

C Service de systématique moléculaire (CNRS-MNHN, UMS2700), Muséum national d’Histoire naturelle, Département Systématique et Evolution, CP 26, 57 Rue Cuvier, F-75231 Paris Cedex 05, France.

D GENOSCOPE, Centre National de Séquençage, 2 rue Gaston Crémieux, CP 5706, 91057 Evry Cedex France.

E Corresponding author. Email: puillandre@mnhn.fr

Invertebrate Systematics 25(4) 322-333 https://doi.org/10.1071/IS11022
Submitted: 25 May 2011  Accepted: 9 November 2011   Published: 22 December 2011

Abstract

The primary purpose of DNA-barcoding projects is to generate an efficient expertise and identification tool. This is an important challenge to the taxonomy of the 21st century, as the demand increases and the expert capacity does not. However, identifying specimens using DNA-barcodes requires a preliminary analysis to relate molecular clusters to available scientific names. Through a case study of the genus Eumunida (Decapoda : Eumunididae), we illustrate how naming molecule-based units, and thus providing an accurate DNA-based identification tool, is facilitated by sequencing type specimens. Using both morphological and unlinked molecular markers (COI and 28S genes), we analysed 230 specimens from 12 geographic areas, covering two-thirds of the known diversity of the genus, including type specimens of 13 species. Most hypotheses of species delimitation are validated, as they correspond to molecular units linked to only one taxonomic name (and vice versa). However, a putative cryptic species is also revealed and three entities previously named as distinct species may in fact belong to a single one, and thus need to be synonymised. Our analyses, which integrate the current naming rules, enhance the α-taxonomy of the genus and provide an effective identification tool based on DNA-barcodes. They illustrate the ability of DNA-barcodes, especially when type specimens are included, to pinpoint where a taxonomic revision is needed.


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