Invertebrate Systematics Invertebrate Systematics Society
Systematics, phylogeny and biogeography
RESEARCH ARTICLE

Two distinct evolutionary lineages of the Astacus leptodactylus species-complex (Decapoda : Astacidae) inferred by phylogenetic analyses

Ivana Maguire A D , Martina Podnar B , Mišel Jelić A , Anamaria Štambuk A , Anne Schrimpf C , Holger Schulz C and Goran Klobučar A
+ Author Affiliations
- Author Affiliations

A University of Zagreb, Faculty of Science, Department of Zoology, Rooseveltov trg 6, 10000 Zagreb, Croatia.

B Croatian Natural History Museum, Demeterova 1, 10000 Zagreb, Croatia.

C Universität Koblenz-Landau, Institute for Environmental Sciences, Fortstrasse 7, 76829 Landau, Germany.

D Corresponding author. Email: imaguire@zg.biol.pmf.hr

Invertebrate Systematics 28(2) 117-123 https://doi.org/10.1071/IS13030
Submitted: 11 June 2013  Accepted: 17 October 2013   Published: 30 May 2014

Abstract

Narrow-clawed crayfish (Astacus leptodactylus Eschscholtz, 1823 species-complex) is one of five European freshwater crayfish species. Even though widely distributed, it hasn’t been frequently studied and its taxonomy and systematics are unresolved. The results of a recent comparative morphometric character study revealed that morphometry of Asian and European populations differ significantly. In this research, for the first time, mitochondrial molecular markers (16S rRNA and COI) were used with the aim of elucidating the phylogenetic relationship between European and Asian populations of the narrow-clawed crayfish. Analyses included crayfish from Croatia, Bulgaria, Armenia, Russia, Poland and Turkey, and three different optimality criteria were applied. Phylogenetic relationships were reconstructed using the COI dataset, as well as the concatenated one (COI + 16S rRNA). For both datasets, congruent topologies were obtained and trees were characterised by the existence of two well supported phylogroups, one that included European populations, and the other Asian. Results indicate the presence of distinct evolutionary lineages within the A. leptodactylus species-complex, and corroborate previous results obtained using morphometric analyses.


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